k 2023

Alterations of AQP4 and Cx43 protein levels in subpial astrocytes (glia limitans superficialis) of the medial prefrontal cortex in reaction to experimental neuropathic pain

DUBOVÝ, Petr; Karolína BRETOVÁ; Anna BAGÓ MAS; Pere BOADAS-VAELLO; Marek JOUKAL et. al.

Základní údaje

Originální název

Alterations of AQP4 and Cx43 protein levels in subpial astrocytes (glia limitans superficialis) of the medial prefrontal cortex in reaction to experimental neuropathic pain

Autoři

DUBOVÝ, Petr; Karolína BRETOVÁ; Anna BAGÓ MAS; Pere BOADAS-VAELLO a Marek JOUKAL

Vydání

THE 14th CONFERENCE OF THE CZECHNEROSCIENCE SOCIETY, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

subpial, astrocytes, cortex, pain

Klíčová slova anglicky

subpial, astrocytes, cortex, pain
Změněno: 27. 11. 2023 15:02, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Subpial astrocytes (SAs) are a specific type of astrocytes covering the CNS surface, including the cortex, forming the glia limitans superficialis (GLS). These astrocytes send cytoplasmic processes into cortical layer I and form an interface between the cortical parenchyma and subpial cerebrospinal fluid (CSF). Therefore, the GLS is considered to be a superficial CSF-brain barrier. The dynamic of AQP4 and Cx43 protein levels in the SAs was investigated in coronal sections of the medial prefrontal cortex (mPFC) from rats and mice in response to sham operation and sciatic nerve compression (SNC) or spinal cord injury (SCI) after different periods of survival. Indirect immunofluorescence staining was performed with primary antibodies against glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), and connexin-43 (Cx43). Immunofluorescence (IF) intensities in the SAs were measured by image analysis and compared between naïve control and operated groups. Our results revealed progressive sending of cytoplasmic processes of the SAs into layer-I of mPFC after both sham operation and SNC or SCI. The SAs displayed distinct immunostaining for AQP4, the intensities of which were reduced in both sham- and SNC- or SCI-operated animals. Simultaneously, increased Cx43-IF intensities were found in both sham- and SNC- or SCI-operated rats and mice over all periods of survival. In summary, we detected the reactivity of the SAs in response to both sham operation and traumatic injury of a peripheral nerve or spinal cord used as neuropathic pain models. The decreased levels of AQP4 in the reactive SAs may indicate impaired bidirectional transport of water and solutes between the cortical parenchyma and subpial CSF through the GLS. In addition, the elevation of Cx43 protein reflects an increased amount of gap junction channels between SA bodies and their cytoplasmic processes, leading to enhanced communication between these astrocytes. These parallel processes are probably associated with changes in ion balance associated with mPFC activity, which plays an important role in the control of anxiety-depressive components of neuropathic pain.

Návaznosti

MUNI/A/1238/2022, interní kód MU
Název: Funkční morfologie: od molekulární biologie ke klinické anatomii 2
Investor: Masarykova univerzita, Funkční morfologie: od molekulární biologie ke klinické anatomii 2