Unravelling Tick-Borne Encephalitis Virus Neutralisation
Mechanisms with Cryo-EM

a 2023

Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK; Lenka ŠMERDOVÁ a Pavel PLEVKA

Základní údaje

Originální název

Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM

Název česky

Odhalení mechanismů neutralizace viru klíšťové encefalitidy pomocí Cryo-EM

Autoři

NEPOVÍMOVÁ, Lucie (203 Česká republika, domácí); Tibor FÜZIK (703 Slovensko, domácí); Lenka ŠMERDOVÁ (203 Česká republika, domácí) a Pavel PLEVKA (203 Česká republika, garant, domácí)

Vydání

VBC Cryo-EM Symposium, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

web symposia

Kód RIV

RIV/00216224:14740/23:00134306

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

TBEV; cryo-EM; antibodies

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2024 11:10, Mgr. Eva Dubská

Anotace

V originále

Tick-borne encephalitis (TBE), caused by the tick-borne encephalitis virus (TBEV), is a severe disease that can lead to fatal central nervous system inflammation. Despite the availability of vaccines, vaccination rates in the most affected areas, such as the Czech Republic, are low. With TBE cases on the rise, the need for targeted treatments is growing. Antibodies have shown promise in a mouse model, where intravenous administration of TBEV-specific antibodies served as a treatment. However, our understanding of the molecular mechanisms behind TBEV neutralisation remains limited.To address this gap, we investigated the interactions between the TBEV Neudörfl strain and two neutralising mouse monoclonal antibodies, IC3 and A4, which bind to distinct domains of the TBEV envelope protein. We purified TBEV from infected tissue culture cells, mixed it with Fab fragments derived from the neutralising antibodies, and vitrified the samples on grids for cryo-electron microscopy. Using single-particle analysis, we solved the structures of the TBEV-Fab complexes from the collected micrographs.By deciphering the molecular basis of TBEV neutralisation by antibodies, we aim to gain insights into the significance of different epitopes on the viral surface. This knowledge may pave the way for the tailored design of therapeutic antibodies or more specific vaccines in the future.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

Citovat

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK; Lenka ŠMERDOVÁ a Pavel PLEVKA. Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM. In VBC Cryo-EM Symposium. 2023.

@proceedings{2344620,
   author = {Nepovímová, Lucie and Füzik, Tibor and Šmerdová, Lenka and Plevka, Pavel},
   booktitle = {VBC Cryo-EM Symposium},
   keywords = {TBEV; cryo-EM; antibodies},
   language = {eng},
   title = {Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM},
   url = {https://www.imp.ac.at/research/cryo-em-symposium},
   year = {2023}
}

TY  - CONF
ID  - 2344620
AU  - Nepovímová, Lucie - Füzik, Tibor - Šmerdová, Lenka - Plevka, Pavel
PY  - 2023
TI  - Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM
KW  - TBEV
KW  - cryo-EM
KW  - antibodies
UR  - https://www.imp.ac.at/research/cryo-em-symposium
N2  - Tick-borne encephalitis (TBE), caused by the tick-borne encephalitis virus (TBEV), is a severe disease that can lead to fatal central nervous system inflammation. Despite the availability of vaccines, vaccination rates in the most affected areas, such as the Czech Republic, are low. With TBE cases on the rise, the need for targeted treatments is growing. Antibodies have shown promise in a mouse model, where intravenous administration of TBEV-specific antibodies served as a treatment. However, our understanding of the molecular mechanisms behind TBEV neutralisation remains limited.To address this gap, we investigated the interactions between the TBEV Neudörfl strain and two neutralising mouse monoclonal antibodies, IC3 and A4, which bind to distinct domains of the TBEV envelope protein. We purified TBEV from infected tissue culture cells, mixed it with Fab fragments derived from the neutralising antibodies, and vitrified the samples on grids for cryo-electron microscopy. Using single-particle analysis, we solved the structures of the TBEV-Fab complexes from the collected micrographs.By deciphering the molecular basis of TBEV neutralisation by antibodies, we aim to gain insights into the significance of different epitopes on the viral surface. This knowledge may pave the way for the tailored design of therapeutic antibodies or more specific vaccines in the future.
ER  -

NEPOVÍMOVÁ, Lucie; Tibor FÜZIK; Lenka ŠMERDOVÁ a Pavel PLEVKA. Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM. In \textit{VBC Cryo-EM Symposium}. 2023.

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