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@article{2347180,
author = {Forinová, Michala and Seidlová, Anna and Pilipenco, Alina and Scott Lynn Jr., Nicholas and Obořilová, Radka and Farka, Zdeněk and Skládal, Petr and Saláková, Alena and Spasovová, Monika and Houska, Milan and Kalhotka, Libor and VaisocherováandLísalová, Hana},
article_number = {November},
doi = {http://dx.doi.org/10.1016/j.crbiot.2023.100166},
keywords = {Food safety; Biosensor; Antifouling coating; Quartz crystal microbalance; Cultivation-based methods; Detection of Staphylococcus aureus; Dairy products},
language = {eng},
issn = {2590-2628},
journal = {Current Research in Biotechnology},
title = {A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products},
url = {https://www.sciencedirect.com/science/article/pii/S2590262823000485},
volume = {6},
year = {2023}
}
TY - JOUR
ID - 2347180
AU - Forinová, Michala - Seidlová, Anna - Pilipenco, Alina - Scott Lynn Jr., Nicholas - Obořilová, Radka - Farka, Zdeněk - Skládal, Petr - Saláková, Alena - Spasovová, Monika - Houska, Milan - Kalhotka, Libor - Vaisocherová-Lísalová, Hana
PY - 2023
TI - A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products
JF - Current Research in Biotechnology
VL - 6
IS - November
SP - 1-6
EP - 1-6
PB - Elsevier
SN - 25902628
KW - Food safety
KW - Biosensor
KW - Antifouling coating
KW - Quartz crystal microbalance
KW - Cultivation-based methods
KW - Detection of Staphylococcus aureus
KW - Dairy products
UR - https://www.sciencedirect.com/science/article/pii/S2590262823000485
N2 - Ensuring dairy product safety demands rapid and precise Staphylococcus aureus (S. aureus) detection. Biosensors show promise, but their performance is often demonstrated in model samples using non-native pathogens and has never been studied towards S. aureus detection in naturally contaminated samples. This study addresses the gap by directly comparing results taken with a novel piezoelectric biosensor, capable of one-step detection, with four conventional cultivation-based methods. Our findings reveal that this biosensor, based on an antifouling nanolayer-coated biochip, exhibits exceptional resistance to biofouling from unprocessed dairy products and is further capable of specific S. aureus detection. Notably, it performed comparably to Petrifilm and Baird-Parker methods but delivered results in only 30 min, bringing a substantial reduction from the 24 h required by cultivation-based techniques. Our study also highlights differences in the performance of cultivation methods when analyzing artificially spiked versus naturally contaminated foods. These findings underline the potential of antifouling biosensors as efficient reliable tools for rapid, cost-effective, point-of-care testing, enhancing fresh dairy product safety and S. aureus detection.
ER -
FORINOVÁ, Michala, Anna SEIDLOVÁ, Alina PILIPENCO, Nicholas SCOTT LYNN JR., Radka OBOŘILOVÁ, Zdeněk FARKA, Petr SKLÁDAL, Alena SALÁKOVÁ, Monika SPASOVOVÁ, Milan HOUSKA, Libor KALHOTKA a Hana VAISOCHEROVÁ-LÍSALOVÁ. A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products. \textit{Current Research in Biotechnology}. Elsevier, 2023, roč.~6, November, s.~1-6. ISSN~2590-2628. Dostupné z: https://dx.doi.org/10.1016/j.crbiot.2023.100166.
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