Contactless conductivity detector as a tool for improving
universality and sensitivity of capillary
electrophoresis-frontal analysis: Proof of concept

J 2024

Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept

BRŽEZICKÁ, Taťána, Hana MLČOCHOVÁ, Zdeněk GLATZ a Lenka KOHÚTOVÁ

Základní údaje

Originální název

Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept

Autoři

BRŽEZICKÁ, Taťána (203 Česká republika, domácí), Hana MLČOCHOVÁ (203 Česká republika, domácí), Zdeněk GLATZ (203 Česká republika, domácí) a Lenka KOHÚTOVÁ (203 Česká republika, garant, domácí)

Vydání

Journal of Separation Science, Weinheim, Wiley, 2024, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/jssc.202300667

UT WoS

001140740000001

Klíčová slova anglicky

binding constant; capillary electrophoresis-frontal analysis; contactless conductivity detection; human serum albumin; salicylic acid; C4D

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 9. 2024 11:30, Mgr. Taťána Bržezická

Anotace

V originále

Drug binding to plasma proteins influences processes such as liberation, adsorption, disposition, metabolism and elimination of drugs, which are thus one of the key steps of a new drug development. As a result, the characterization of drug protein interactions is an essential part of these time- and money-consuming processes. It is important to determine not only the binding strength and the stoichiometry of interaction, but also the binding site of a drug on a protein molecule, because two drugs with the same binding site can mutually affect free drug concentration. Capillary electrophoresis-frontal analysis with mobility shift affinity capillary electrophoresis is one of the most used affinity CE methods for the characterization of these interactions. In this study, a well-known sensitivity problem of most capillary electrophoresis-frontal analyses using UV detection is solved by its combination with contactless conductivity detection, which provided 6-fold lower limits of quantitation and detection. Binding parameters of the human serum albumin salicylic acid model affinity pair were evaluated by this newly developed approach and by the classical approach with UV detection primarily used for their mutual comparison. The results of both approaches agreed well, and are also in agreement with literature data obtained using different techniques.

Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

MUNI/A/1313/2022, interní kód MU

Název: Podpora biochemického výzkumu v roce 2023

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2023

NU23-08-00229, projekt VaV

Název: Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie

Investor: Ministerstvo zdravotnictví ČR, Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie, Podprogram 1 - standardní

Citovat

BRŽEZICKÁ, Taťána, Hana MLČOCHOVÁ, Zdeněk GLATZ a Lenka KOHÚTOVÁ. Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept. Journal of Separation Science. Weinheim: Wiley, 2024, roč. 47, č. 1, s. 1-8. ISSN 1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.202300667.

@article{2349181,
   author = {Bržezická, Taťána and Mlčochová, Hana and Glatz, Zdeněk and Kohútová, Lenka},
   article_location = {Weinheim},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/jssc.202300667},
   keywords = {binding constant; capillary electrophoresis-frontal analysis; contactless conductivity detection; human serum albumin; salicylic acid; C4D},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300667},
   volume = {47},
   year = {2024}
}

TY  - JOUR
ID  - 2349181
AU  - Bržezická, Taťána - Mlčochová, Hana - Glatz, Zdeněk - Kohútová, Lenka
PY  - 2024
TI  - Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept
JF  - Journal of Separation Science
VL  - 47
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Wiley
SN  - 16159306
KW  - binding constant
KW  - capillary electrophoresis-frontal analysis
KW  - contactless conductivity detection
KW  - human serum albumin
KW  - salicylic acid
KW  - C4D
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300667
N2  - Drug binding to plasma proteins influences processes such as liberation, adsorption, disposition, metabolism and elimination of drugs, which are thus one of the key steps of a new drug development. As a result, the characterization of drug protein interactions is an essential part of these time- and money-consuming processes. It is important to determine not only the binding strength and the stoichiometry of interaction, but also the binding site of a drug on a protein molecule, because two drugs with the same binding site can mutually affect free drug concentration. Capillary electrophoresis-frontal analysis with mobility shift affinity capillary electrophoresis is one of the most used affinity CE methods for the characterization of these interactions. In this study, a well-known sensitivity problem of most capillary electrophoresis-frontal analyses using UV detection is solved by its combination with contactless conductivity detection, which provided 6-fold lower limits of quantitation and detection. Binding parameters of the human serum albumin salicylic acid model affinity pair were evaluated by this newly developed approach and by the classical approach with UV detection primarily used for their mutual comparison. The results of both approaches agreed well, and are also in agreement with literature data obtained using different techniques.
ER  -

BRŽEZICKÁ, Taťána, Hana MLČOCHOVÁ, Zdeněk GLATZ a Lenka KOHÚTOVÁ. Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept. \textit{Journal of Separation Science}. Weinheim: Wiley, 2024, roč.~47, č.~1, s.~1-8. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.202300667.

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