Immunohistochemical localization of laminin-1 in the acellular
nerve grafts is associated with migrating Schwann cells which
display corresponding integrin receptors

J 1999

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA et. al.

Základní údaje

Originální název

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

Název česky

Imunohistochemická analýza lamininu v acelulárním nervovém štěpu

Autoři

DUBOVÝ, Petr (203 Česká republika, garant), Ivana SVÍŽENSKÁ (203 Česká republika), Radim JANČÁLEK (203 Česká republika), Ilona KLUSÁKOVÁ (203 Česká republika), Ladislav HOUŠŤAVA (203 Česká republika) a Pavel HANINEC (203 Česká republika)

Vydání

General Physiology and Biophysics, Bratislava, Veda, 1999, 0231-5882

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.400

Kód RIV

RIV/00216224:14110/99:00001800

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration

Štítky

acellular nerve graft, Integrins, Laminin-1, Schwann cell migration

Změněno: 17. 6. 2009 12:15, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.

Česky

Imunohistochemická detekce lamininu-1, collagen-IV, a6 a b1 řetězce integrinů byla studována v acelulárním segmentu nervu

Citovat

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA a Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. General Physiology and Biophysics. Bratislava: Veda, 1999, roč. 18, č. 1, s. 63-65. ISSN 0231-5882.

@article{234931,
   author = {Dubový, Petr and Svíženská, Ivana and Jančálek, Radim and Klusáková, Ilona and Houšťava, Ladislav and Haninec, Pavel},
   article_location = {Bratislava},
   article_number = {1},
   keywords = {Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration},
   language = {eng},
   issn = {0231-5882},
   journal = {General Physiology and Biophysics},
   title = {Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors},
   volume = {18},
   year = {1999}
}

TY  - JOUR
ID  - 234931
AU  - Dubový, Petr - Svíženská, Ivana - Jančálek, Radim - Klusáková, Ilona - Houšťava, Ladislav - Haninec, Pavel
PY  - 1999
TI  - Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
JF  - General Physiology and Biophysics
VL  - 18
IS  - 1
SP  - 63
EP  - 63
PB  - Veda
SN  - 02315882
KW  - Acellular nerve graft
KW  - Integrins
KW  - Laminin-1
KW  - Schwann cell migration
N2  - The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
ER  -

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA a Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. \textit{General Physiology and Biophysics}. Bratislava: Veda, 1999, roč.~18, č.~1, s.~63-65. ISSN~0231-5882.

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