| PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI, Jiří KROUPA, Pavel HOUŠKA, Barbora ADAMOVÁ, Jarmila NAVRÁTILOVÁ a Viktor KANICKÝ. Nanoparticle tag counting for tissue imaging using single-particle ICP MS. In European Winter Conference on Plasma Spectrochemistry 2023. 2023. |
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@proceedings{2351159,
author = {Preisler, Jan and Stiborek, Marek and Jindřichová, Lenka and Meliorisová, Stanislava and Bednařík, Antonín and Prysiazhnyi, Vadym and Kroupa, Jiří and Houška, Pavel and Adamová, Barbora and Navrátilová, Jarmila and Kanický, Viktor},
booktitle = {European Winter Conference on Plasma Spectrochemistry 2023},
keywords = {nanoparticles; imaging; single particle inductively coupled plasma mass spectrometry; SP ICP MS; laser ablation},
language = {eng},
title = {Nanoparticle tag counting for tissue imaging using single-particle ICP MS},
year = {2023}
}
TY - CONF
ID - 2351159
AU - Preisler, Jan - Stiborek, Marek - Jindřichová, Lenka - Meliorisová, Stanislava - Bednařík, Antonín - Prysiazhnyi, Vadym - Kroupa, Jiří - Houška, Pavel - Adamová, Barbora - Navrátilová, Jarmila - Kanický, Viktor
PY - 2023
TI - Nanoparticle tag counting for tissue imaging using single-particle ICP MS
KW - nanoparticles
KW - imaging
KW - single particle inductively coupled plasma mass spectrometry
KW - SP ICP MS
KW - laser ablation
N2 - We report a new laser ablation technique employing a pulsed 2 940 nm laser and a simple ablation cell for digital mapping biomarkers in tissues using metal nanoparticle tags. Unlike the conventional laser ablation systems, where nanoparticles are ablated, we are able to desorb intact nanoparticles and count them in the single-particle mode using inductively coupled plasma mass spectrometry. The method is demonstrated on monitoring proliferating cells in 3D aggregates of human colorectal carcinoma cells. Precise counting of the tags in each pixel generates sharp distribution maps of a proliferation biomarker in the tissue. A mass spectrometer detects up to 83% nanoparticles of a selected element with a single particle detection limit. Advantageously, the desorption of nanoparticles from regions outside the tissue is strongly suppressed.
ER -
PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI, Jiří KROUPA, Pavel HOUŠKA, Barbora ADAMOVÁ, Jarmila NAVRÁTILOVÁ a Viktor KANICKÝ. Nanoparticle tag counting for tissue imaging using single-particle ICP MS. In \textit{European Winter Conference on Plasma Spectrochemistry 2023}. 2023.
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