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@article{2352317,
author = {Chatzigeorgiou, Sofia and Jílková, Jana and Korecká, Lucie and Janyšková, Radka and Hermannová, Martina and Šimek, Matej and Čožíková, Dagmar and Slováková, Marcela and Bílková, Zuzana and Bobek, Jan and Černý, Zbyněk and Čihák, Matous and Velebný, Vladimír},
article_number = {October},
doi = {http://dx.doi.org/10.1016/j.carbpol.2023.121078},
keywords = {Beta-glucuronidase; Oligosaccharides; Hyaluronan; Immobilization; Bacteroides fragilis},
language = {eng},
issn = {0144-8617},
journal = {Carbohydrate Polymers},
title = {Preparation of hyaluronan oligosaccharides by a prokaryotic beta-glucuronidase: Characterization of free and immobilized forms of the enzyme},
url = {https://doi.org/10.1016/j.carbpol.2023.121078},
volume = {317},
year = {2023}
}
TY - JOUR
ID - 2352317
AU - Chatzigeorgiou, Sofia - Jílková, Jana - Korecká, Lucie - Janyšková, Radka - Hermannová, Martina - Šimek, Matej - Čožíková, Dagmar - Slováková, Marcela - Bílková, Zuzana - Bobek, Jan - Černý, Zbyněk - Čihák, Matous - Velebný, Vladimír
PY - 2023
TI - Preparation of hyaluronan oligosaccharides by a prokaryotic beta-glucuronidase: Characterization of free and immobilized forms of the enzyme
JF - Carbohydrate Polymers
VL - 317
IS - October
SP - 1-12
EP - 1-12
PB - Elsevier
SN - 01448617
KW - Beta-glucuronidase
KW - Oligosaccharides
KW - Hyaluronan
KW - Immobilization
KW - Bacteroides fragilis
UR - https://doi.org/10.1016/j.carbpol.2023.121078
N2 - Popularity of hyaluronan (HA) in the cosmetics and pharmaceutical industries, led to the investigation and development of new HA-based materials, with enzymes playing a key role. Beta-D-glucuronidases catalyze the hydrolysis of a beta-D-glucuronic acid residue from the non-reducing end of various substrates. However, lack of specificity towards HA for most beta-D-glucuronidases, in addition to the high cost and low purity of those active on HA, have prevented their widespread application. In this study, we investigated a recombinant beta-glucuronidase from Bacteroides fragilis (rBfGUS). We demonstrated the rBfGUS's activity on native, modified, and derivatized HA oligosaccharides (oHAs). Using chromogenic beta-glucuronidase substrate and oHAs, we characterized the enzyme's optimal conditions and kinetic parameters. Additionally, we evaluated rBfGUS's activity towards oHAs of various sizes and types. To increase reusability and ensure the preparation of enzyme-free oHA products, rBfGUS was immobilized on two types of magnetic macroporous bead cellulose particles. Both immobilized forms of rBfGUS demonstrated suitable operational and storage stabilities, and their activity pa-rameters were comparable to the free form. Our findings suggest that native and derivatized oHAs can be pre-pared using this bacterial beta-glucuronidase, and a novel biocatalyst with enhanced operational parameters has been developed with a potential for industrial use.
ER -
CHATZIGEORGIOU, Sofia, Jana JÍLKOVÁ, Lucie KORECKÁ, Radka JANYŠKOVÁ, Martina HERMANNOVÁ, Matej ŠIMEK, Dagmar ČOŽÍKOVÁ, Marcela SLOVÁKOVÁ, Zuzana BÍLKOVÁ, Jan BOBEK, Zbyněk ČERNÝ, Matous ČIHÁK a Vladimír VELEBNÝ. Preparation of hyaluronan oligosaccharides by a prokaryotic beta-glucuronidase: Characterization of free and immobilized forms of the enzyme. \textit{Carbohydrate Polymers}. Elsevier, 2023, roč.~317, October, s.~1-12. ISSN~0144-8617. Dostupné z: https://dx.doi.org/10.1016/j.carbpol.2023.121078.
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