Detection of metallothionein as a melanoma marker by LA-ICP-MS
combined with sample pretreatment by using magnetic particles
coated with an imprinted polymeric layer

J 2023

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

VEVERKOVA, Kristyna, Kristyna PAVELICOVA, Marcela VLCNOVSKA, Markéta VEJVODOVÁ, Vratislav HORAK et. al.

Základní údaje

Originální název

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Autoři

VEVERKOVA, Kristyna, Kristyna PAVELICOVA, Marcela VLCNOVSKA, Markéta VEJVODOVÁ (203 Česká republika, domácí), Vratislav HORAK, Viktor KANICKÝ (203 Česká republika, domácí), Vojtech ADAM, Tomáš VACULOVIČ (203 Česká republika, domácí) a Marketa VACULOVICOVA

Vydání

Journal of analytical atomic spectrometry, Cambridge, Royal Society of Chemistry, 2023, 0267-9477

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00132618

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1039/d3ja00065f

UT WoS

001022916300001

Klíčová slova anglicky

NANOPARTICLES; OVEREXPRESSION; EXPRESSION

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2024 20:56, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu;M) was 63.3% (46.5 & mu;M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin.

Návaznosti

GA20-02203S, projekt VaV

Název: Analýza tkáňové odpovědi na inhalaci nanočástic kovů a mechanismus jejich čištění

Investor: Grantová agentura ČR, Analysis of tissue response to metal nanoparticles inhalation and mechanisms of their clearance

MUNI/A/1298/2022, interní kód MU

Název: Základní a aplikovaný výzkum a vývoj metod chemické a fyzikálně chemické analýzy pro studium přírody a pokročilé technologie

Investor: Masarykova univerzita, Základní a aplikovaný výzkum a vývoj metod chemické a fyzikálně chemické analýzy pro studium přírody a pokročilé technologie

Citovat

VEVERKOVA, Kristyna, Kristyna PAVELICOVA, Marcela VLCNOVSKA, Markéta VEJVODOVÁ, Vratislav HORAK, Viktor KANICKÝ, Vojtech ADAM, Tomáš VACULOVIČ a Marketa VACULOVICOVA. Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer. Journal of analytical atomic spectrometry. Cambridge: Royal Society of Chemistry, 2023, roč. 38, č. 8, s. 1618-1625. ISSN 0267-9477. Dostupné z: https://dx.doi.org/10.1039/d3ja00065f.

@article{2352402,
   author = {Veverkova, Kristyna and Pavelicova, Kristyna and Vlcnovska, Marcela and Vejvodová, Markéta and Horak, Vratislav and Kanický, Viktor and Adam, Vojtech and Vaculovič, Tomáš and Vaculovicova, Marketa},
   article_location = {Cambridge},
   article_number = {8},
   doi = {http://dx.doi.org/10.1039/d3ja00065f},
   keywords = {NANOPARTICLES; OVEREXPRESSION; EXPRESSION},
   language = {eng},
   issn = {0267-9477},
   journal = {Journal of analytical atomic spectrometry},
   title = {Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer},
   url = {https://pubs.rsc.org/en/content/articlelanding/2023/JA/D3JA00065F},
   volume = {38},
   year = {2023}
}

TY  - JOUR
ID  - 2352402
AU  - Veverkova, Kristyna - Pavelicova, Kristyna - Vlcnovska, Marcela - Vejvodová, Markéta - Horak, Vratislav - Kanický, Viktor - Adam, Vojtech - Vaculovič, Tomáš - Vaculovicova, Marketa
PY  - 2023
TI  - Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer
JF  - Journal of analytical atomic spectrometry
VL  - 38
IS  - 8
SP  - 1618-1625
EP  - 1618-1625
PB  - Royal Society of Chemistry
SN  - 02679477
KW  - NANOPARTICLES
KW  - OVEREXPRESSION
KW  - EXPRESSION
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/JA/D3JA00065F
N2  - Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu;M) was 63.3% (46.5 & mu;M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin.
ER  -

VEVERKOVA, Kristyna, Kristyna PAVELICOVA, Marcela VLCNOVSKA, Markéta VEJVODOVÁ, Vratislav HORAK, Viktor KANICKÝ, Vojtech ADAM, Tomáš VACULOVIČ a Marketa VACULOVICOVA. Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer. \textit{Journal of analytical atomic spectrometry}. Cambridge: Royal Society of Chemistry, 2023, roč.~38, č.~8, s.~1618-1625. ISSN~0267-9477. Dostupné z: https://dx.doi.org/10.1039/d3ja00065f.

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