Unveiling the nanotoxicological aspects of Se nanomaterials
differing in size and morphology

J 2023

Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology

STEPANKOVA, Hana, Hana MICHALKOVA, Zbynek SPLICHAL, Lukas RICHTERA, Pavel SVEC et. al.

Basic information

Original name

Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology

Authors

STEPANKOVA, Hana, Hana MICHALKOVA, Zbynek SPLICHAL, Lukas RICHTERA, Pavel SVEC, Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Martin KORMUNDA, Simona REX, Vojtech ADAM and Zbynek HEGER (guarantor)

Edition

BIOACTIVE MATERIALS, PEOPLES R CHINA, KEAI PUBLISHING LTD, 2023, 2452-199X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

China

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 18.900 in 2022

RIV identification code

RIV/00216224:14310/23:00132629

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.bioactmat.2022.06.014

UT WoS

000830012200002

Keywords in English

Aspect ratio; Biocompatibility; Nanotoxicology; Nanorods

Abstract

V originále

Although the general concept of nanotechnology relies on exploitation of size-dependent properties of nano -scaled materials, the relation between the size/morphology of nanoparticles with their biological activity re-mains not well understood. Therefore, we aimed at investigating the biological activity of Se nanoparticles, one of the most promising candidates of nanomaterials for biomedicine, possessing the same crystal structure, but differing in morphology (nanorods vs. spherical particles) and aspect ratios (AR, 11.5 vs. 22.3 vs. 1.0) in human cells and BALB/c mice. Herein, we report that in case of nanorod-shaped Se nanomaterials, AR is a critical factor describing their cytotoxicity and biocompatibility. However, spherical nanoparticles (AR 1.0) do not fit this statement and exhibit markedly higher cytotoxicity than lower-AR Se nanorods. Beside of cytotoxicity, we also show that morphology and size substantially affect the uptake and intracellular fate of Se nanomaterials. In line with in vitro data, in vivo i.v. administration of Se nanomaterials revealed the highest toxicity for higher-AR nanorods followed by spherical nanoparticles and lower-AR nanorods. Moreover, we revealed that Se nano -materials are able to alter intracellular redox homeostasis, and affect the acidic intracellular vesicles and cyto-skeletal architecture in a size-and morphology-dependent manner. Although the tested nanoparticles were produced from the similar sources, their behavior differs markedly, since each type is promising for several various application scenarios, and the presented testing protocol could serve as a concept standardizing the biological relevance of the size and morphology of the various types of nanomaterials and nanoparticles.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90124, large research infrastructures

Name: NanoEnviCz II

90127, large research infrastructures

Name: CIISB II

Citovat

STEPANKOVA, Hana, Hana MICHALKOVA, Zbynek SPLICHAL, Lukas RICHTERA, Pavel SVEC, Tomáš VACULOVIČ, Jan PŘIBYL, Martin KORMUNDA, Simona REX, Vojtech ADAM and Zbynek HEGER. Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology. BIOACTIVE MATERIALS. PEOPLES R CHINA: KEAI PUBLISHING LTD, 2023, vol. 20, FEB, p. 489-500. ISSN 2452-199X. Available from: https://dx.doi.org/10.1016/j.bioactmat.2022.06.014.

@article{2352478,
   author = {Stepankova, Hana and Michalkova, Hana and Splichal, Zbynek and Richtera, Lukas and Svec, Pavel and Vaculovič, Tomáš and Přibyl, Jan and Kormunda, Martin and Rex, Simona and Adam, Vojtech and Heger, Zbynek},
   article_location = {PEOPLES R CHINA},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.1016/j.bioactmat.2022.06.014},
   keywords = {Aspect ratio; Biocompatibility; Nanotoxicology; Nanorods},
   language = {eng},
   issn = {2452-199X},
   journal = {BIOACTIVE MATERIALS},
   title = {Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology},
   url = {https://sciencedirect.com/science/article/pii/S2452199X22002808?via%3Dihub},
   volume = {20},
   year = {2023}
}

TY  - JOUR
ID  - 2352478
AU  - Stepankova, Hana - Michalkova, Hana - Splichal, Zbynek - Richtera, Lukas - Svec, Pavel - Vaculovič, Tomáš - Přibyl, Jan - Kormunda, Martin - Rex, Simona - Adam, Vojtech - Heger, Zbynek
PY  - 2023
TI  - Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology
JF  - BIOACTIVE MATERIALS
VL  - 20
IS  - FEB
SP  - 489-500
EP  - 489-500
PB  - KEAI PUBLISHING LTD
SN  - 2452199X
KW  - Aspect ratio
KW  - Biocompatibility
KW  - Nanotoxicology
KW  - Nanorods
UR  - https://sciencedirect.com/science/article/pii/S2452199X22002808?via%3Dihub
N2  - Although the general concept of nanotechnology relies on exploitation of size-dependent properties of nano -scaled materials, the relation between the size/morphology of nanoparticles with their biological activity re-mains not well understood. Therefore, we aimed at investigating the biological activity of Se nanoparticles, one of the most promising candidates of nanomaterials for biomedicine, possessing the same crystal structure, but differing in morphology (nanorods vs. spherical particles) and aspect ratios (AR, 11.5 vs. 22.3 vs. 1.0) in human cells and BALB/c mice. Herein, we report that in case of nanorod-shaped Se nanomaterials, AR is a critical factor describing their cytotoxicity and biocompatibility. However, spherical nanoparticles (AR 1.0) do not fit this statement and exhibit markedly higher cytotoxicity than lower-AR Se nanorods. Beside of cytotoxicity, we also show that morphology and size substantially affect the uptake and intracellular fate of Se nanomaterials. In line with in vitro data, in vivo i.v. administration of Se nanomaterials revealed the highest toxicity for higher-AR nanorods followed by spherical nanoparticles and lower-AR nanorods. Moreover, we revealed that Se nano -materials are able to alter intracellular redox homeostasis, and affect the acidic intracellular vesicles and cyto-skeletal architecture in a size-and morphology-dependent manner. Although the tested nanoparticles were produced from the similar sources, their behavior differs markedly, since each type is promising for several various application scenarios, and the presented testing protocol could serve as a concept standardizing the biological relevance of the size and morphology of the various types of nanomaterials and nanoparticles.
ER  -

STEPANKOVA, Hana, Hana MICHALKOVA, Zbynek SPLICHAL, Lukas RICHTERA, Pavel SVEC, Tomáš VACULOVIČ, Jan PŘIBYL, Martin KORMUNDA, Simona REX, Vojtech ADAM and Zbynek HEGER. Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology. \textit{BIOACTIVE MATERIALS}. PEOPLES R CHINA: KEAI PUBLISHING LTD, 2023, vol.~20, FEB, p.~489-500. ISSN~2452-199X. Available from: https://dx.doi.org/10.1016/j.bioactmat.2022.06.014.

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