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@article{2355287,
author = {Smolková, Denisa and Moravcová, Dana and Lavická, Jana},
article_location = {Weinheim},
article_number = {1},
doi = {http://dx.doi.org/10.1002/jssc.202300705},
keywords = {capillary electrophoresis; cationic labeling; glycans; oligosaccharides; solid-phase extraction},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300705},
volume = {47},
year = {2024}
}
TY - JOUR
ID - 2355287
AU - Smolková, Denisa - Moravcová, Dana - Lavická, Jana
PY - 2024
TI - Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis
JF - Journal of Separation Science
VL - 47
IS - 1
SP - 1-11
EP - 1-11
PB - Wiley-VCH Verlag GmbH
SN - 16159306
KW - capillary electrophoresis
KW - cationic labeling
KW - glycans
KW - oligosaccharides
KW - solid-phase extraction
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300705
N2 - The sample preparation including labeling and clean-up represents a key analytical step in the analysis of oligosaccharides and glycans by either chromatographic or electrophoretic separation methods. Although the majority of labeling has been performed by neutral and/or negatively charged tags, the introduction of a positive charge into the saccharide molecule can significantly improve the analysis, especially with mass spectrometry detection. In this work, we present the evaluation of five solid-phase extraction sorbents differing in extraction chemistry for the clean-up and concentration of positively labeled maltooligosaccharides from the reaction mixtures. Maltooligosaccharides containing four to seven glucose units were labeled by cationic tags (2-aminoethyl)trimethylammonium chloride and (carboxymethyl)trimethylammonium chloride hydrazide and the extraction conditions were optimized followed by electrophoretic analysis with conductivity detection. The effects of the solid-phase extraction sorbent chemistry, extraction conditions, and sample composition are discussed. All tested sorbents were capable of cleaning up maltooligosaccharides from the reaction mixtures to some extent after optimization of the solid-phase extraction procedure (51.9%-98.9% recovery). The best-rated amide-based sorbent was used to process the sample of N-linked glycans enzymatically released from ribonuclease B.
ER -
SMOLKOVÁ, Denisa, Dana MORAVCOVÁ a Jana LAVICKÁ. Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis. \textit{Journal of Separation Science}. Weinheim: Wiley-VCH Verlag GmbH, 2024, roč.~47, č.~1, s.~1-11. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.202300705.
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