Characterization of the conserved features of the NSE6 subunit
of the Physcomitrium patens PpSMC5/6 complex

J 2023

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR et. al.

Základní údaje

Originální název

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex

Název česky

Charakterizace konzervovaných vlastností NSE6 podjednotky Physcomitrium patens komplexu PpSMC5/6

Autoři

LELKES, Edit (703 Slovensko, domácí), Jitka JEMELKOVÁ (203 Česká republika, domácí), Marcela HOLÁ (203 Česká republika), Barbora ŠTEFANOVIE (703 Slovensko, domácí), Peter KOLESÁR (703 Slovensko, domácí), Radka VÁGNEROVÁ (203 Česká republika), Eva DVOŘÁK TOMAŠTÍKOVÁ (203 Česká republika), Aleš PEČINKA (203 Česká republika), Karel J. ANGELIS (203 Česká republika) a Jan PALEČEK (203 Česká republika, garant, domácí)

Vydání

PLANT JOURNAL, ENGLAND, WILEY, 2023, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00132792

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/tpj.16282

UT WoS

000995683100001

Klíčová slova anglicky

SMC5/6 complex; NSE5/SNI1/SLF1/SIMC1; NSE6/ASAP1/SLF2/KRE29; CANIN domain; DNA damage repair; plant development; Physcomitrium patens

Štítky

protein-protein interactions, rivok, SMC5-6 complex

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 1. 2024 10:43, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Structural maintenance of chromosomes (SMC) complexes are molecular machines ensuring chromatin organization at higher levels. They play direct roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of long-armed SMC, kleisin, and kleisin-associated subunits. Additional factors, like NSE6 within SMC5/6, bind to SMC core complexes and regulate their activities. In the human HsNSE6/SLF2, we recently identified a new CANIN domain. Here we tracked down its sequence homology to lower plants, selected the bryophyte Physcomitrium patens, and analyzed PpNSE6 protein-protein interactions to explore its conservation in detail. We identified a previously unrecognized core sequence motif conserved from yeasts to humans within the NSE6 CANIN domain. This motif mediates the interaction between NSE6 and its NSE5 partner in yeasts and plants. In addition, the CANIN domain and its preceding PpNSE6 sequences bind both PpSMC5 and PpSMC6 arms. Interestingly, we mapped the PpNSE6-binding site at the PpSMC5 arm right next to the PpNSE2-binding surface. The position of NSE6 at SMC arms suggests its role in the regulation of SMC5/6 dynamics. Consistent with the regulatory role of NSE6 subunits, Ppnse6 mutant lines were viable and sensitive to the DNA-damaging drug bleomycin and lost a large portion of rDNA copies. These moss mutants also exhibited reduced growth and developmental aberrations. Altogether, our data showed the conserved function of the NSE6 subunit and architecture of the SMC5/6 complex across species.

Návaznosti

GA20-05095S, projekt VaV

Název: Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA (Akronym: SMC5/6)

Investor: Grantová agentura ČR, Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

MUNI/R/1142/2021, interní kód MU

Název: Visualization of the 3D structure of chromatin

Investor: Masarykova univerzita, Visualization of the 3D structure of chromatin, CAREER RESTART

MUNI/R/1262/2022, interní kód MU

Název: Vztah mezi opravou DNA a transkripcí

Investor: Masarykova univerzita, Vztah mezi opravou DNA a transkripcí, CAREER RESTART

Citovat

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR, Radka VÁGNEROVÁ, Eva DVOŘÁK TOMAŠTÍKOVÁ, Aleš PEČINKA, Karel J. ANGELIS a Jan PALEČEK. Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex. PLANT JOURNAL. ENGLAND: WILEY, 2023, roč. 115, č. 4, s. 1084-1099. ISSN 0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.16282.

@article{2356460,
   author = {Lelkes, Edit and Jemelková, Jitka and Holá, Marcela and Štefanovie, Barbora and Kolesár, Peter and Vágnerová, Radka and Dvořák Tomaštíková, Eva and Pečinka, Aleš and Angelis, Karel J. and Paleček, Jan},
   article_location = {ENGLAND},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/tpj.16282},
   keywords = {SMC5/6 complex; NSE5/SNI1/SLF1/SIMC1; NSE6/ASAP1/SLF2/KRE29; CANIN domain; DNA damage repair; plant development; Physcomitrium patens},
   language = {eng},
   issn = {0960-7412},
   journal = {PLANT JOURNAL},
   title = {Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.16282},
   volume = {115},
   year = {2023}
}

TY  - JOUR
ID  - 2356460
AU  - Lelkes, Edit - Jemelková, Jitka - Holá, Marcela - Štefanovie, Barbora - Kolesár, Peter - Vágnerová, Radka - Dvořák Tomaštíková, Eva - Pečinka, Aleš - Angelis, Karel J. - Paleček, Jan
PY  - 2023
TI  - Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex
JF  - PLANT JOURNAL
VL  - 115
IS  - 4
SP  - 1084-1099
EP  - 1084-1099
PB  - WILEY
SN  - 09607412
KW  - SMC5/6 complex
KW  - NSE5/SNI1/SLF1/SIMC1
KW  - NSE6/ASAP1/SLF2/KRE29
KW  - CANIN domain
KW  - DNA damage repair
KW  - plant development
KW  - Physcomitrium patens
UR  - https://onlinelibrary.wiley.com/doi/10.1111/tpj.16282
N2  - Structural maintenance of chromosomes (SMC) complexes are molecular machines ensuring chromatin organization at higher levels. They play direct roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of long-armed SMC, kleisin, and kleisin-associated subunits. Additional factors, like NSE6 within SMC5/6, bind to SMC core complexes and regulate their activities. In the human HsNSE6/SLF2, we recently identified a new CANIN domain. Here we tracked down its sequence homology to lower plants, selected the bryophyte Physcomitrium patens, and analyzed PpNSE6 protein-protein interactions to explore its conservation in detail. We identified a previously unrecognized core sequence motif conserved from yeasts to humans within the NSE6 CANIN domain. This motif mediates the interaction between NSE6 and its NSE5 partner in yeasts and plants. In addition, the CANIN domain and its preceding PpNSE6 sequences bind both PpSMC5 and PpSMC6 arms. Interestingly, we mapped the PpNSE6-binding site at the PpSMC5 arm right next to the PpNSE2-binding surface. The position of NSE6 at SMC arms suggests its role in the regulation of SMC5/6 dynamics. Consistent with the regulatory role of NSE6 subunits, Ppnse6 mutant lines were viable and sensitive to the DNA-damaging drug bleomycin and lost a large portion of rDNA copies. These moss mutants also exhibited reduced growth and developmental aberrations. Altogether, our data showed the conserved function of the NSE6 subunit and architecture of the SMC5/6 complex across species.
ER  -

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR, Radka VÁGNEROVÁ, Eva DVOŘÁK TOMAŠTÍKOVÁ, Aleš PEČINKA, Karel J. ANGELIS a Jan PALEČEK. Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex. \textit{PLANT JOURNAL}. ENGLAND: WILEY, 2023, roč.~115, č.~4, s.~1084-1099. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.16282.

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