The ultrastructural nature of human oocytes' cytoplasmic
abnormalities and the role of cytoskeleton dysfunction

J 2023

The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction

TATÍČKOVÁ, Martina, Zuzana TREBICHALSKÁ, Drahomíra KYJOVSKÁ, Pavel OTEVŘEL, Soňa KLOUDOVÁ et. al.

Základní údaje

Originální název

The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction

Autoři

TATÍČKOVÁ, Martina (203 Česká republika, domácí), Zuzana TREBICHALSKÁ (703 Slovensko, domácí), Drahomíra KYJOVSKÁ (203 Česká republika), Pavel OTEVŘEL, Soňa KLOUDOVÁ (203 Česká republika) a Zuzana HOLUBCOVÁ (203 Česká republika, domácí)

Vydání

F&S science, New York, Elsevier, 2023, 2666-335X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/23:00132848

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.xfss.2023.09.002

Klíčová slova anglicky

Cytoskeleton; dysmorphism; electron microscopy; human oocyte; oocyte abnormalities

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 10:53, Ing. Martina Blahová

Anotace

V originále

Objective: To investigate the structural bases of human oocytes' cytoplasmic abnormalities and the causative mechanism of their emergence. Knowledge of an abnormal oocyte's intracellular organization is vital to establishing reliable criteria for clinical evaluation of oocyte morphology. Design: Laboratory-based study on experimental material provided by a private assisted reproduction clinic. Setting: University laboratory and imaging center. Patients: A total of 105 women undergoing hormonal stimulation for in vitro fertilization (IVF) donated their spare oocytes for this study. Interventions: Transmission electron microscopy (TEM) was used to analyze the fine morphology of 22 dysmorphic IVF oocytes exhibiting different types of cytoplasmic irregularities, namely, refractile bodies; centrally located cytoplasmic granularity (CLCG); smooth endoplasmic reticulum (SER) disc; and vacuoles. A total of 133 immature oocytes were exposed to cytoskeleton-targeting compounds or matured in control conditions, and their morphology was examined using fluorescent and electron microscopy. Main outcome measures: The ultrastructural morphology of dysmorphic oocytes was analyzed. Drug-treated oocytes had their maturation efficiency, chromosome-microtubule configurations, and fine intracellular morphology examined. Results: TEM revealed ultrastructural characteristics of common oocyte aberrations and indicated that excessive organelle clustering was the underlying cause of 2 of the studied morphotypes. Inhibition experiments showed that disruption of actin, not microtubules, allows for inordinate aggregation of subcellular structures, resembling the ultrastructural pattern seen in morphologically abnormal oocytes retrieved in IVF cycles. These results imply that actin serves as a regulator of organelle distribution during human oocyte maturation. Conclusion: The ultrastructural analogy between dysmorphic oocytes and oocytes, in which actin network integrity was perturbed, suggests that dysfunction of the actin cytoskeleton might be implicated in generating common cytoplasmic aberrations. Knowledge of human oocytes' inner workings and the origin of morphological abnormalities is a step forward to a more objective oocyte quality assessment in IVF practice.

Návaznosti

GJ19-14990Y, projekt VaV

Název: Ultrastrukturní znaky kvality lidských oocytů (Akronym: lidské oocyty)

Investor: Grantová agentura ČR, Ultrastrukturní znaky kvality lidských oocytů.

MUNI/A/1301/2022, interní kód MU

Název: Zdroje pro tkáňové inženýrství 13

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 13

MUNI/A/1398/2021, interní kód MU

Název: Zdroje pro tkáňové inženýrství 12 (Akronym: TissueEng 12)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 12

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

TATÍČKOVÁ, Martina, Zuzana TREBICHALSKÁ, Drahomíra KYJOVSKÁ, Pavel OTEVŘEL, Soňa KLOUDOVÁ a Zuzana HOLUBCOVÁ. The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction. F&S science. New York: Elsevier, 2023, roč. 4, č. 4, s. 267-278. ISSN 2666-335X. Dostupné z: https://dx.doi.org/10.1016/j.xfss.2023.09.002.

@article{2357976,
   author = {Tatíčková, Martina and Trebichalská, Zuzana and Kyjovská, Drahomíra and Otevřel, Pavel and Kloudová, Soňa and Holubcová, Zuzana},
   article_location = {New York},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.xfss.2023.09.002},
   keywords = {Cytoskeleton; dysmorphism; electron microscopy; human oocyte; oocyte abnormalities},
   language = {eng},
   issn = {2666-335X},
   journal = {F&S science},
   title = {The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction},
   url = {https://www.sciencedirect.com/science/article/pii/S2666335X23000502?via%3Dihub},
   volume = {4},
   year = {2023}
}

TY  - JOUR
ID  - 2357976
AU  - Tatíčková, Martina - Trebichalská, Zuzana - Kyjovská, Drahomíra - Otevřel, Pavel - Kloudová, Soňa - Holubcová, Zuzana
PY  - 2023
TI  - The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction
JF  - F&S science
VL  - 4
IS  - 4
SP  - 267-278
EP  - 267-278
PB  - Elsevier
SN  - 2666335X
KW  - Cytoskeleton
KW  - dysmorphism
KW  - electron microscopy
KW  - human oocyte
KW  - oocyte abnormalities
UR  - https://www.sciencedirect.com/science/article/pii/S2666335X23000502?via%3Dihub
N2  - Objective: To investigate the structural bases of human oocytes' cytoplasmic abnormalities and the causative mechanism of their emergence. Knowledge of an abnormal oocyte's intracellular organization is vital to establishing reliable criteria for clinical evaluation of oocyte morphology. Design: Laboratory-based study on experimental material provided by a private assisted reproduction clinic. Setting: University laboratory and imaging center. Patients: A total of 105 women undergoing hormonal stimulation for in vitro fertilization (IVF) donated their spare oocytes for this study. Interventions: Transmission electron microscopy (TEM) was used to analyze the fine morphology of 22 dysmorphic IVF oocytes exhibiting different types of cytoplasmic irregularities, namely, refractile bodies; centrally located cytoplasmic granularity (CLCG); smooth endoplasmic reticulum (SER) disc; and vacuoles. A total of 133 immature oocytes were exposed to cytoskeleton-targeting compounds or matured in control conditions, and their morphology was examined using fluorescent and electron microscopy. Main outcome measures: The ultrastructural morphology of dysmorphic oocytes was analyzed. Drug-treated oocytes had their maturation efficiency, chromosome-microtubule configurations, and fine intracellular morphology examined. Results: TEM revealed ultrastructural characteristics of common oocyte aberrations and indicated that excessive organelle clustering was the underlying cause of 2 of the studied morphotypes. Inhibition experiments showed that disruption of actin, not microtubules, allows for inordinate aggregation of subcellular structures, resembling the ultrastructural pattern seen in morphologically abnormal oocytes retrieved in IVF cycles. These results imply that actin serves as a regulator of organelle distribution during human oocyte maturation. Conclusion: The ultrastructural analogy between dysmorphic oocytes and oocytes, in which actin network integrity was perturbed, suggests that dysfunction of the actin cytoskeleton might be implicated in generating common cytoplasmic aberrations. Knowledge of human oocytes' inner workings and the origin of morphological abnormalities is a step forward to a more objective oocyte quality assessment in IVF practice.
ER  -

TATÍČKOVÁ, Martina, Zuzana TREBICHALSKÁ, Drahomíra KYJOVSKÁ, Pavel OTEVŘEL, Soňa KLOUDOVÁ a Zuzana HOLUBCOVÁ. The ultrastructural nature of human oocytes' cytoplasmic abnormalities and the role of cytoskeleton dysfunction. \textit{F\&{}S science}. New York: Elsevier, 2023, roč.~4, č.~4, s.~267-278. ISSN~2666-335X. Dostupné z: https://dx.doi.org/10.1016/j.xfss.2023.09.002.

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