| PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ, Ondřej HELIA, Klara PANZAROVA, Jiří FAJKUS, Zbyněk ZDRÁHAL, Gabriela LOCHMANOVÁ and Miloslava FOJTOVÁ. Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors. PLANTS-BASEL. BASEL: MDPI, 2023, vol. 12, No 24, p. 1-16. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants12244177. |
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@article{2359157,
author = {Pírek, Pavlína and Kryštofová, Karolína and Kováčová, Ingrid and Kromerová, Anna and Zachová, Dagmar and Helia, Ondřej and Panzarova, Klara and Fajkus, Jiří and Zdráhal, Zbyněk and Lochmanová, Gabriela and Fojtová, Miloslava},
article_location = {BASEL},
article_number = {24},
doi = {http://dx.doi.org/10.3390/plants12244177},
keywords = {Arabidopsis thaliana; callus formation; epigenetics; histone posttranslational modifications; mass spectrometry; trichostatin A; sodium butyrate},
language = {eng},
issn = {2223-7747},
journal = {PLANTS-BASEL},
title = {Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors},
url = {https://www.mdpi.com/2223-7747/12/24/4177},
volume = {12},
year = {2023}
}
TY - JOUR
ID - 2359157
AU - Pírek, Pavlína - Kryštofová, Karolína - Kováčová, Ingrid - Kromerová, Anna - Zachová, Dagmar - Helia, Ondřej - Panzarova, Klara - Fajkus, Jiří - Zdráhal, Zbyněk - Lochmanová, Gabriela - Fojtová, Miloslava
PY - 2023
TI - Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors
JF - PLANTS-BASEL
VL - 12
IS - 24
SP - 1-16
EP - 1-16
PB - MDPI
SN - 22237747
KW - Arabidopsis thaliana
KW - callus formation
KW - epigenetics
KW - histone posttranslational modifications
KW - mass spectrometry
KW - trichostatin A
KW - sodium butyrate
UR - https://www.mdpi.com/2223-7747/12/24/4177
N2 - The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of Arabidopsis thaliana seedlings. Increased levels of the histone H3.3 variant were found to be the major and most prominent feature of 20-day calli, associated with chromatin relaxation. The methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate, and trichostatin A. Each HDACi affected the state of post-translational histone modifications in a specific manner; NaB-treated calli were epigenetically more similar to root-derived calli, and TSA-treated calli resembled shoot-derived calli.
ER -
PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ, Ondřej HELIA, Klara PANZAROVA, Jiří FAJKUS, Zbyněk ZDRÁHAL, Gabriela LOCHMANOVÁ and Miloslava FOJTOVÁ. Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors. \textit{PLANTS-BASEL}. BASEL: MDPI, 2023, vol.~12, No~24, p.~1-16. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants12244177.
|
