Sulfation of furcellaran and its effect on hemocompatibility in
vitro

J 2024

Sulfation of furcellaran and its effect on hemocompatibility in vitro

ŠTĚPÁNKOVÁ, Kateřina, Kadir OZALTIN, Radka GOREJOVÁ, Hana DOUDOVÁ, Eva BERGEROVÁ et. al.

Základní údaje

Originální název

Sulfation of furcellaran and its effect on hemocompatibility in vitro

Autoři

ŠTĚPÁNKOVÁ, Kateřina (203 Česká republika), Kadir OZALTIN (203 Česká republika), Radka GOREJOVÁ (703 Slovensko), Hana DOUDOVÁ (203 Česká republika), Eva BERGEROVÁ (203 Česká republika), Iveta MASKALOVÁ (703 Slovensko), Monika STUPAVSKÁ (203 Česká republika, domácí), Pavel SŤAHEL (203 Česká republika, domácí), David TRUNEC (203 Česká republika, garant, domácí), Jana PELKOVÁ (203 Česká republika), Miran MOZETIČ (705 Slovinsko) a Marián LEHOCKÝ (203 Česká republika)

Vydání

International Journal of Biological Macromolecules, Amsterdam, Elsevier Science BV, 2024, 0141-8130

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.200 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijbiomac.2023.128840

UT WoS

001137688700001

Klíčová slova anglicky

Anticoagulant; Furcellaran; Hemocompatibility; Platelet adhesion; Seaweed polysaccharide; Sulfation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 1. 2024 10:11, Mgr. Marie Šípková, DiS.

Anotace

V originále

In this study, furcellaran (FUR) obtained from Furcellaria lumbricalis was firstly employed for sulfation via various methods, including SO3-pyridine (SO3∙Py) complex in different aprotic solvents, chlorosulfonic acid and sulfuric acid with a "coupling" reagent N,N'-Dicyclohexylcarbodiimide. Structural characterization through FT-IR, GPC, XPS and elemental analyses confirmed the successful synthesis of 6-O-sulfated FUR derivates characterized by varying degrees of sulfation (DS) ranging from 0.15 to 0.91 and molecular weight (Mw) spanning from12.5kDa to 2.7kDa. In vitro clotting assays, partial thromboplastin time (aPTT), thrombin time (TT), and prothrombin time (PT) underscored the essential role of sulfate esters in conferring anticoagulant activity whereas FUR prepared via chlorosulfonic acid with DS of 0.91 reached 311.4s in aPPT showing almost 4-fold higher anticoagulant activity than native FUR at the concentration 2mg/mL. MTT test showed all tested samples decreased cell viability in a dose dependent manner while all of them are non-cytotoxic up to the concentration of 0.1mg/mL. Furthermore, sulfated derivates deposited onto polyethylene terephthalate surface presented substantial decrease in platelet adhesion, as well as absence of the most activated platelet stages. These findings support the pivotal role of O-6 FUR sulfates in enhancing hemocompatibility and provide valuable insights for a comparative assessment of effective sulfating approaches.

Citovat

ŠTĚPÁNKOVÁ, Kateřina, Kadir OZALTIN, Radka GOREJOVÁ, Hana DOUDOVÁ, Eva BERGEROVÁ, Iveta MASKALOVÁ, Monika STUPAVSKÁ, Pavel SŤAHEL, David TRUNEC, Jana PELKOVÁ, Miran MOZETIČ a Marián LEHOCKÝ. Sulfation of furcellaran and its effect on hemocompatibility in vitro. International Journal of Biological Macromolecules. Amsterdam: Elsevier Science BV, 2024, roč. 258, č. 1, s. 128840-128851. ISSN 0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2023.128840.

@article{2360019,
   author = {Štěpánková, Kateřina and Ozaltin, Kadir and Gorejová, Radka and Doudová, Hana and Bergerová, Eva and Maskalová, Iveta and Stupavská, Monika and Sťahel, Pavel and Trunec, David and Pelková, Jana and Mozetič, Miran and Lehocký, Marián},
   article_location = {Amsterdam},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.ijbiomac.2023.128840},
   keywords = {Anticoagulant; Furcellaran; Hemocompatibility; Platelet adhesion; Seaweed polysaccharide; Sulfation},
   language = {eng},
   issn = {0141-8130},
   journal = {International Journal of Biological Macromolecules},
   title = {Sulfation of furcellaran and its effect on hemocompatibility in vitro},
   url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179757931&doi=10.1016%2fj.ijbiomac.2023.128840&partnerID=40&md5=97d93c2318f6f237be724461ad620fd2},
   volume = {258},
   year = {2024}
}

TY  - JOUR
ID  - 2360019
AU  - Štěpánková, Kateřina - Ozaltin, Kadir - Gorejová, Radka - Doudová, Hana - Bergerová, Eva - Maskalová, Iveta - Stupavská, Monika - Sťahel, Pavel - Trunec, David - Pelková, Jana - Mozetič, Miran - Lehocký, Marián
PY  - 2024
TI  - Sulfation of furcellaran and its effect on hemocompatibility in vitro
JF  - International Journal of Biological Macromolecules
VL  - 258
IS  - 1
SP  - 128840
EP  - 128840
PB  - Elsevier Science BV
SN  - 01418130
KW  - Anticoagulant
KW  - Furcellaran
KW  - Hemocompatibility
KW  - Platelet adhesion
KW  - Seaweed polysaccharide
KW  - Sulfation
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179757931&doi=10.1016%2fj.ijbiomac.2023.128840&partnerID=40&md5=97d93c2318f6f237be724461ad620fd2
N2  - In this study, furcellaran (FUR) obtained from Furcellaria lumbricalis was firstly employed for sulfation via various methods, including SO3-pyridine (SO3∙Py) complex in different aprotic solvents, chlorosulfonic acid and sulfuric acid with a "coupling" reagent N,N'-Dicyclohexylcarbodiimide. Structural characterization through FT-IR, GPC, XPS and elemental analyses confirmed the successful synthesis of 6-O-sulfated FUR derivates characterized by varying degrees of sulfation (DS) ranging from 0.15 to 0.91 and molecular weight (Mw) spanning from12.5kDa to 2.7kDa. In vitro clotting assays, partial thromboplastin time (aPTT), thrombin time (TT), and prothrombin time (PT) underscored the essential role of sulfate esters in conferring anticoagulant activity whereas FUR prepared via chlorosulfonic acid with DS of 0.91 reached 311.4s in aPPT showing almost 4-fold higher anticoagulant activity than native FUR at the concentration 2mg/mL. MTT test showed all tested samples decreased cell viability in a dose dependent manner while all of them are non-cytotoxic up to the concentration of 0.1mg/mL. Furthermore, sulfated derivates deposited onto polyethylene terephthalate surface presented substantial decrease in platelet adhesion, as well as absence of the most activated platelet stages. These findings support the pivotal role of O-6 FUR sulfates in enhancing hemocompatibility and provide valuable insights for a comparative assessment of effective sulfating approaches.
ER  -

ŠTĚPÁNKOVÁ, Kateřina, Kadir OZALTIN, Radka GOREJOVÁ, Hana DOUDOVÁ, Eva BERGEROVÁ, Iveta MASKALOVÁ, Monika STUPAVSKÁ, Pavel SŤAHEL, David TRUNEC, Jana PELKOVÁ, Miran MOZETIČ a Marián LEHOCKÝ. Sulfation of furcellaran and its effect on hemocompatibility in vitro. \textit{International Journal of Biological Macromolecules}. Amsterdam: Elsevier Science BV, 2024, roč.~258, č.~1, s.~128840-128851. ISSN~0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2023.128840.

Podrobný výpis o publikaci