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@article{2360019,
author = {Štěpánková, Kateřina and Ozaltin, Kadir and Gorejová, Radka and Doudová, Hana and Bergerová, Eva and Maskalová, Iveta and Stupavská, Monika and Sťahel, Pavel and Trunec, David and Pelková, Jana and Mozetič, Miran and Lehocký, Marián},
article_location = {Amsterdam},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.ijbiomac.2023.128840},
keywords = {Anticoagulant; Furcellaran; Hemocompatibility; Platelet adhesion; Seaweed polysaccharide; Sulfation},
language = {eng},
issn = {0141-8130},
journal = {International Journal of Biological Macromolecules},
title = {Sulfation of furcellaran and its effect on hemocompatibility in vitro},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179757931&doi=10.1016%2fj.ijbiomac.2023.128840&partnerID=40&md5=97d93c2318f6f237be724461ad620fd2},
volume = {258},
year = {2024}
}
TY - JOUR
ID - 2360019
AU - Štěpánková, Kateřina - Ozaltin, Kadir - Gorejová, Radka - Doudová, Hana - Bergerová, Eva - Maskalová, Iveta - Stupavská, Monika - Sťahel, Pavel - Trunec, David - Pelková, Jana - Mozetič, Miran - Lehocký, Marián
PY - 2024
TI - Sulfation of furcellaran and its effect on hemocompatibility in vitro
JF - International Journal of Biological Macromolecules
VL - 258
IS - 1
SP - 128840
EP - 128840
PB - Elsevier Science BV
SN - 01418130
KW - Anticoagulant
KW - Furcellaran
KW - Hemocompatibility
KW - Platelet adhesion
KW - Seaweed polysaccharide
KW - Sulfation
UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179757931&doi=10.1016%2fj.ijbiomac.2023.128840&partnerID=40&md5=97d93c2318f6f237be724461ad620fd2
N2 - In this study, furcellaran (FUR) obtained from Furcellaria lumbricalis was firstly employed for sulfation via various methods, including SO3-pyridine (SO3∙Py) complex in different aprotic solvents, chlorosulfonic acid and sulfuric acid with a "coupling" reagent N,N'-Dicyclohexylcarbodiimide. Structural characterization through FT-IR, GPC, XPS and elemental analyses confirmed the successful synthesis of 6-O-sulfated FUR derivates characterized by varying degrees of sulfation (DS) ranging from 0.15 to 0.91 and molecular weight (Mw) spanning from12.5kDa to 2.7kDa. In vitro clotting assays, partial thromboplastin time (aPTT), thrombin time (TT), and prothrombin time (PT) underscored the essential role of sulfate esters in conferring anticoagulant activity whereas FUR prepared via chlorosulfonic acid with DS of 0.91 reached 311.4s in aPPT showing almost 4-fold higher anticoagulant activity than native FUR at the concentration 2mg/mL. MTT test showed all tested samples decreased cell viability in a dose dependent manner while all of them are non-cytotoxic up to the concentration of 0.1mg/mL. Furthermore, sulfated derivates deposited onto polyethylene terephthalate surface presented substantial decrease in platelet adhesion, as well as absence of the most activated platelet stages. These findings support the pivotal role of O-6 FUR sulfates in enhancing hemocompatibility and provide valuable insights for a comparative assessment of effective sulfating approaches.
ER -
ŠTĚPÁNKOVÁ, Kateřina, Kadir OZALTIN, Radka GOREJOVÁ, Hana DOUDOVÁ, Eva BERGEROVÁ, Iveta MASKALOVÁ, Monika STUPAVSKÁ, Pavel SŤAHEL, David TRUNEC, Jana PELKOVÁ, Miran MOZETIČ and Marián LEHOCKÝ. Sulfation of furcellaran and its effect on hemocompatibility in vitro. \textit{International Journal of Biological Macromolecules}. Amsterdam: Elsevier Science BV, 2024, vol.~258, No~1, p.~128840-128851. ISSN~0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2023.128840.
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