Changes in gene expression in pressure ulcers debrided by
different approaches - a pilot study

J 2023

Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ et. al.

Základní údaje

Originální název

Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study

Autoři

SAMADIAN, Amir (364 Írán, domácí), Monika KRATOCHVÍLOVÁ (203 Česká republika, domácí), Alica HOKYNKOVÁ (703 Slovensko, domácí), Petr ŠÍN (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí), Petr ŠTĚPKA (203 Česká republika, domácí), Andrea POKORNÁ (203 Česká republika, domácí) a Petr BABULA (203 Česká republika, domácí)

Vydání

Physiological research, Praha, Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.100 v roce 2022

Kód RIV

RIV/00216224:14110/23:00134692

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.33549/physiolres.935222

UT WoS

001198072000009

Klíčová slova anglicky

Pressure ulcers; Debridement; Wound healing; Oxidative stress; Gene expression

Štítky

14110229, 14110515, 14110611, rivok

Příznaky

Recenzováno

Změněno: 29. 4. 2024 13:05, Mgr. Tereza Miškechová

Anotace

V originále

Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.

Návaznosti

NU21-09-00541, projekt VaV

Název: Role oxidativního stresu při hojení dekubitů u pacientů s míšní lézí

Investor: Ministerstvo zdravotnictví ČR, Role oxidativního stresu při hojení dekubitů u pacientů s míšní lézí, Podprogram 1 - standardní

Citovat

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ, Petr ŠTĚPKA, Andrea POKORNÁ a Petr BABULA. Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study. Physiological research. Praha: Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, roč. 72, Supplementum 5, s. "S535"-"S542", 8 s. ISSN 0862-8408. Dostupné z: https://dx.doi.org/10.33549/physiolres.935222.

@article{2360027,
   author = {Samadian, Amir and Kratochvílová, Monika and Hokynková, Alica and Šín, Petr and Nováková, Marie and Štěpka, Petr and Pokorná, Andrea and Babula, Petr},
   article_location = {Praha},
   article_number = {Supplementum 5},
   doi = {http://dx.doi.org/10.33549/physiolres.935222},
   keywords = {Pressure ulcers; Debridement; Wound healing; Oxidative stress; Gene expression},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological research},
   title = {Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study},
   url = {https://www.biomed.cas.cz/physiolres/pdf/72/72_S535.pdf},
   volume = {72},
   year = {2023}
}

TY  - JOUR
ID  - 2360027
AU  - Samadian, Amir - Kratochvílová, Monika - Hokynková, Alica - Šín, Petr - Nováková, Marie - Štěpka, Petr - Pokorná, Andrea - Babula, Petr
PY  - 2023
TI  - Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study
JF  - Physiological research
VL  - 72
IS  - Supplementum 5
SP  - "S535"-"S542"
EP  - "S535"-"S542"
PB  - Institute of Physiology of the Czechoslovak Academy of Sciences
SN  - 08628408
KW  - Pressure ulcers
KW  - Debridement
KW  - Wound healing
KW  - Oxidative stress
KW  - Gene expression
UR  - https://www.biomed.cas.cz/physiolres/pdf/72/72_S535.pdf
N2  - Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.
ER  -

SAMADIAN, Amir, Monika KRATOCHVÍLOVÁ, Alica HOKYNKOVÁ, Petr ŠÍN, Marie NOVÁKOVÁ, Petr ŠTĚPKA, Andrea POKORNÁ a Petr BABULA. Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study. \textit{Physiological research}. Praha: Institute of Physiology of the Czechoslovak Academy of Sciences, 2023, roč.~72, Supplementum 5, s.~''S535''-''S542'', 8 s. ISSN~0862-8408. Dostupné z: https://dx.doi.org/10.33549/physiolres.935222.

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