Perspectives on antimicrobial properties of Paulownia tomentosa
Steud. fruit products in the control of Staphylococcus aureus
infections

J 2024

Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections

ŠKOVRANOVÁ, Gabriela, Lenka MOLČANOVÁ, Blaž JUG, Dina JUG, Anja KLANČNIK et. al.

Základní údaje

Originální název

Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections

Autoři

ŠKOVRANOVÁ, Gabriela, Lenka MOLČANOVÁ, Blaž JUG, Dina JUG, Anja KLANČNIK, Sonja SMOLE-MOŽINA, Jakub TREML, Magda TUŠEK ŽNIDARIČ a Alice SYCHROVÁ

Vydání

JOURNAL OF ETHNOPHARMACOLOGY, IRELAND, ELSEVIER IRELAND LTD, 2024, 0378-8741

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.400 v roce 2022

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.1016/j.jep.2023.117461

Klíčová slova česky

alternativní kontrola stafylokoků, antimikrobiální aktivita, geranylované flavonoidy, inhibice quorum sensing, produkty plodů Paulownia tomentosa,

Klíčová slova anglicky

alternative staphylococcal control, antimicrobial activity, geranylated flavonoids, Paulownia tomentosa fruit products, quorum sensing inhibition

Štítky

ÚMF, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 1. 2024 17:17, PharmDr. Gabriela Škovranová, Ph.D.

Anotace

V originále

Introduction: Paulownia tomentosa Steud. (P. tomentosa) is a medium-sized tree traditionally used in Chinese folk medicine for the treatment of infectious diseases. It is a rich source of prenylated phenolic compounds that have been extensively studied for their promising biological activities. Aim of the study: Due to the increasing development of antibiotic resistance, our study investigated plant-derived natural products from the fruits of P. tomentosa that could control Staphylococcus aureus infections with novel targets/modes of action and reduce antimicrobial resistance. Materials and methods: The ethanolic extract was fractionated and detected by liquid chromatography. The antistaphylococcal effects of the plant formulations were studied in detail in vitro by various biological methods, including microdilution methods for minimum inhibitory concentration (MIC), the checkerboard titration technique for synergy assay, fluorescence measurements for membrane disruption experiments, autoinducer-2-mediated bioassay for quorum sensing inhibition, and counting of colony-forming units for relative adhesion. Morphology was examined by transmission electron microscopy. Results: Total ethanolic extract and chloroform fraction showed MICs of 128 and 32 μg/mL, respectively. Diplacol, diplacone, and 3′-O-methyl-5′-hydroxydiplacone inhibited S. aureus growth in the range of 8–16 μg/mL. Synergistic potential was shown in combination with mupirocin and fusidic acid. The ethanolic extract and the chloroform fraction destroyed the cell membranes by 91.61% and 79.46%, respectively, while the pure compounds were less active. The ethanolic extract and the pure compounds reduced the number of adhered cells to 47.33–10.26% compared to the untreated control. All tested plant formulations, except diplacone, inhibited quorum sensing of S. aureus. Transmission electron microscopy showed deformation of S. aureus cells. Conclusions: The products from the fruit of P. tomentosa showed antimicrobial properties against S. aureus alone and in combination with antibiotics. By affecting intracellular targets, geranylated flavonoids proposed novel approaches in the control of staphylococcal infections.

Návaznosti

MUNI/A/1212/2022, interní kód MU

Název: Synergizmus prenylovaných flavonoidov s topickými antibiotikami v boji proti stafylokokovým infekciám rán

Investor: Masarykova univerzita, Synergizmus prenylovaných flavonoidov s topickými antibiotikami v boji proti stafylokokovým infekciám rán

Citovat

ŠKOVRANOVÁ, Gabriela, Lenka MOLČANOVÁ, Blaž JUG, Dina JUG, Anja KLANČNIK, Sonja SMOLE-MOŽINA, Jakub TREML, Magda TUŠEK ŽNIDARIČ a Alice SYCHROVÁ. Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections. JOURNAL OF ETHNOPHARMACOLOGY. IRELAND: ELSEVIER IRELAND LTD, 2024, roč. 2024, March, s. 117461. ISSN 0378-8741. Dostupné z: https://dx.doi.org/10.1016/j.jep.2023.117461.

@article{2363004,
   author = {Škovranová, Gabriela and Molčanová, Lenka and Jug, Blaž and Jug, Dina and Klančnik, Anja and SmoleandMožina, Sonja and Treml, Jakub and Tušek Žnidarič, Magda and Sychrová, Alice},
   article_location = {IRELAND},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.jep.2023.117461},
   keywords = {alternative staphylococcal control, antimicrobial activity, geranylated flavonoids, Paulownia tomentosa fruit products, quorum sensing inhibition},
   language = {eng},
   issn = {0378-8741},
   journal = {JOURNAL OF ETHNOPHARMACOLOGY},
   title = {Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections},
   volume = {2024},
   year = {2024}
}

TY  - JOUR
ID  - 2363004
AU  - Škovranová, Gabriela - Molčanová, Lenka - Jug, Blaž - Jug, Dina - Klančnik, Anja - Smole-Možina, Sonja - Treml, Jakub - Tušek Žnidarič, Magda - Sychrová, Alice
PY  - 2024
TI  - Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections
JF  - JOURNAL OF ETHNOPHARMACOLOGY
VL  - 2024
IS  - March
SP  - 117461
EP  - 117461
PB  - ELSEVIER IRELAND LTD
SN  - 03788741
KW  - alternative staphylococcal control, antimicrobial activity, geranylated flavonoids, Paulownia tomentosa fruit products, quorum sensing inhibition
N2  - Introduction: Paulownia tomentosa Steud. (P. tomentosa) is a medium-sized tree traditionally used in Chinese folk medicine for the treatment of infectious diseases. It is a rich source of prenylated phenolic compounds that have been extensively studied for their promising biological activities. Aim of the study: Due to the increasing development of antibiotic resistance, our study investigated plant-derived natural products from the fruits of P. tomentosa that could control Staphylococcus aureus infections with novel targets/modes of action and reduce antimicrobial resistance. Materials and methods: The ethanolic extract was fractionated and detected by liquid chromatography. The antistaphylococcal effects of the plant formulations were studied in detail in vitro by various biological methods, including microdilution methods for minimum inhibitory concentration (MIC), the checkerboard titration technique for synergy assay, fluorescence measurements for membrane disruption experiments, autoinducer-2-mediated bioassay for quorum sensing inhibition, and counting of colony-forming units for relative adhesion. Morphology was examined by transmission electron microscopy. Results: Total ethanolic extract and chloroform fraction showed MICs of 128 and 32 μg/mL, respectively. Diplacol, diplacone, and 3′-O-methyl-5′-hydroxydiplacone inhibited S. aureus growth in the range of 8–16 μg/mL. Synergistic potential was shown in combination with mupirocin and fusidic acid. The ethanolic extract and the chloroform fraction destroyed the cell membranes by 91.61% and 79.46%, respectively, while the pure compounds were less active. The ethanolic extract and the pure compounds reduced the number of adhered cells to 47.33–10.26% compared to the untreated control. All tested plant formulations, except diplacone, inhibited quorum sensing of S. aureus. Transmission electron microscopy showed deformation of S. aureus cells. Conclusions: The products from the fruit of P. tomentosa showed antimicrobial properties against S. aureus alone and in combination with antibiotics. By affecting intracellular targets, geranylated flavonoids proposed novel approaches in the control of staphylococcal infections.
ER  -

ŠKOVRANOVÁ, Gabriela, Lenka MOLČANOVÁ, Blaž JUG, Dina JUG, Anja KLANČNIK, Sonja SMOLE-MOŽINA, Jakub TREML, Magda TUŠEK ŽNIDARIČ a Alice SYCHROVÁ. Perspectives on antimicrobial properties of Paulownia tomentosa Steud. fruit products in the control of Staphylococcus aureus infections. \textit{JOURNAL OF ETHNOPHARMACOLOGY}. IRELAND: ELSEVIER IRELAND LTD, 2024, roč.~2024, March, s.~117461. ISSN~0378-8741. Dostupné z: https://dx.doi.org/10.1016/j.jep.2023.117461.

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