Detection of protein biomarkers using lateral flow immunoassays
based on photon-upconversion nanoparticles

a 2023

Detection of protein biomarkers using lateral flow immunoassays based on photon-upconversion nanoparticles

MACHÁČOVÁ, Eliška, Julian BRANDMEIER, Ekaterina MAKHNEVA, Antonín HLAVÁČEK, Hans-Heiner GORRIS et. al.

Základní údaje

Originální název

Detection of protein biomarkers using lateral flow immunoassays based on photon-upconversion nanoparticles

Autoři

MACHÁČOVÁ, Eliška, Julian BRANDMEIER, Ekaterina MAKHNEVA, Antonín HLAVÁČEK, Hans-Heiner GORRIS a Zdeněk FARKA

Vydání

CECE 2023, 2023

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Klíčová slova česky

foton-upkonverzní nanočástice, UCNP, imunostanovení, lidský sérový albumin, HSA, diagnostický proužek, LFIA

Klíčová slova anglicky

photon-upconversion nanoparticle, UCNP, immunoassay, human serum albumin, HSA, diagnostic strip, LFIA

Příznaky

Mezinárodní význam

Změněno: 26. 1. 2024 10:11, Mgr. Eliška Macháčová

Anotace

V originále

Rapid and sensitive detection of protein biomarkers is essential for the early diagnosis and prevention of different diseases. Due to the fast and easy analysis, lateral flow immunoassays (LFIAs) are currently becoming one of the most popular forms of tests for the detection of various analytes. The high selectivity of LFIAs is given by the high specificity of antibodies. Furthermore, little to no laboratory equipment is required to perform these assays, making them suitable for point-of-care testing (PoCT). Gold nanoparticles (AuNPs) are generally used as a label for LFIA analysis, allowing for naked-eye readout. However, even though AuNP-based LFIAs are highly convenient for PoCT testing, the sensitivity is often insufficient to detect low-abundance biomarkers. Thus, different kinds of nanomaterials are being studied for use as alternative labels. Our work focused on the application of photon-upconversion nanoparticles (UCNPs) as a label in LFIAs. UCNPs are lanthanide-doped nanocrystals exhibiting anti-Stokes luminescence; they can be excited by the near-infrared laser and detected in the visible region without optical background interferences in biological samples. UCNPs composed of NaYF4 doped with Yb3+ and Er3+ were synthesized and conjugated with specific antibodies via copper-catalyzed click chemistry. Such conjugates enabled the development of LFIA assays for the detection of protein biomarkers human serum albumin (HSA) and prostate-specific antigen (PSA). Several optimization steps were carried out. The biggest impact was observed in the case of selection of suitable membranes for designing the LFIA strips, addition of of methanol to buffer for immobilization of antibodies, and addition of sucrose to buffer for incubation of UCNP conjugate. So far, UCNP-based LFIA has been successfully used for detecting HSA and PSA from buffers, proving that UCNPs are a convenient alternative to AuNPs. Moreover, the preliminary data demonstrate great potential for real sample analysis in various body fluids (e.g., blood serum or urine). The UCNP-based LFIAs can thus enable the sensitive detection of protein biomarkers and other clinically relevant analytes, combining simplicity of the assay with high sensitivity.

Návaznosti

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Citovat

MACHÁČOVÁ, Eliška, Julian BRANDMEIER, Ekaterina MAKHNEVA, Antonín HLAVÁČEK, Hans-Heiner GORRIS a Zdeněk FARKA. Detection of protein biomarkers using lateral flow immunoassays based on photon-upconversion nanoparticles. In CECE 2023. 2023.

@proceedings{2365860,
   author = {Macháčová, Eliška and Brandmeier, Julian and Makhneva, Ekaterina and Hlaváček, Antonín and Gorris, HansandHeiner and Farka, Zdeněk},
   booktitle = {CECE 2023},
   keywords = {photon-upconversion nanoparticle, UCNP, immunoassay, human serum albumin, HSA, diagnostic strip, LFIA},
   title = {Detection of protein biomarkers using lateral flow immunoassays based on photon-upconversion nanoparticles},
   year = {2023}
}

TY  - CONF
ID  - 2365860
AU  - Macháčová, Eliška - Brandmeier, Julian - Makhneva, Ekaterina - Hlaváček, Antonín - Gorris, Hans-Heiner - Farka, Zdeněk
PY  - 2023
TI  - Detection of protein biomarkers using lateral flow immunoassays based on photon-upconversion nanoparticles
KW  - photon-upconversion nanoparticle, UCNP, immunoassay, human serum albumin, HSA, diagnostic strip, LFIA
N2  - Rapid and sensitive detection of protein biomarkers is essential for the early diagnosis and prevention of different diseases. Due to the fast and easy analysis, lateral flow immunoassays (LFIAs) are currently becoming one of the most popular forms of tests for the detection of various analytes. The high selectivity of LFIAs is given by the high specificity of antibodies. Furthermore, little to no laboratory equipment is required to perform these assays, making them suitable for point-of-care testing (PoCT). Gold nanoparticles (AuNPs) are generally used as a label for LFIA analysis, allowing for naked-eye readout. However, even though AuNP-based LFIAs are highly convenient for PoCT testing, the sensitivity is often insufficient to detect low-abundance biomarkers. Thus, different kinds of nanomaterials are being studied for use as alternative labels. Our work focused on the application of photon-upconversion nanoparticles (UCNPs) as a label in LFIAs. UCNPs are lanthanide-doped nanocrystals exhibiting anti-Stokes luminescence; they can be excited by the near-infrared laser and detected in the visible region without optical background interferences in biological samples. UCNPs composed of NaYF4 doped with Yb3+ and Er3+ were synthesized and conjugated with specific antibodies via copper-catalyzed click chemistry. Such conjugates enabled the development of LFIA assays for the detection of protein biomarkers human serum albumin (HSA) and prostate-specific antigen (PSA). Several optimization steps were carried out. The biggest impact was observed in the case of selection of suitable membranes for designing the LFIA strips, addition of of methanol to buffer for immobilization of antibodies, and addition of sucrose to buffer for incubation of UCNP conjugate. So far, UCNP-based LFIA has been successfully used for detecting HSA and PSA from buffers, proving that UCNPs are a convenient alternative to AuNPs. Moreover, the preliminary data demonstrate great potential for real sample analysis in various body fluids (e.g., blood serum or urine). The UCNP-based LFIAs can thus enable the sensitive detection of protein biomarkers and other clinically relevant analytes, combining simplicity of the assay with high sensitivity.
ER  -

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