Detection of prostate-specific antigen utilizing immunomagnetic
assay with upconversion nanoparticles

a 2023

Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles

SKLENÁROVÁ, Dorota, Ekaterina MAKHNEVA, Julian BRANDMEIER, Hans-Heiner GORRIS, Zdeněk FARKA et. al.

Základní údaje

Originální název

Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles

Autoři

SKLENÁROVÁ, Dorota, Ekaterina MAKHNEVA, Julian BRANDMEIER, Hans-Heiner GORRIS a Zdeněk FARKA

Vydání

23rd Joint meeting of Biophysical Chemists and Electrochemists 2023, 2023

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Klíčová slova česky

proteinové biomarkery, rakovina prostaty, imunostanovení, magnetické mikročástice, upkonverzní nanočástice

Klíčová slova anglicky

protein biomarkers, prostate cancer, immunoassays, magnetic microparticles, upconversion nanoparticles

Změněno: 26. 1. 2024 11:40, Mgr. Dorota Sklenárová

Anotace

V originále

Prostate cancer is the leading cause of death within the male population worldwide caused by oncologic diseases. The glycoprotein prostate-specific antigen (PSA) produced by the prostate is its most important biomarker. While blood serum concentrations up to 2.5 ng/mL are considered normal and can be generally detected by conventional immunoassays, more sensitive detection schemes are essential for early-stage disease diagnosis. Immunochemical methods, such as the enzyme-linked immunosorbent assay (ELISA), are considered the gold standard for PSA detection. However, there are several limitations that render ELISA insufficient regarding the sensitivity required for early-stage diagnosis.1 Photon-upconversion nanoparticles (UCNPs) are luminescent nanocrystals consisting of an inorganic matrix doped with lanthanide ions. They exhibit anti-Stokes luminescence (the ability to convert low-energy excitation to higher energy emission), thus avoiding optical background interference. When conjugated with biorecognition molecules (e.g., antibodies or streptavidin), UCNPs can be used as a sensitive label in various immunoassay formats, including microtiter plate-based upconversion-linked immunosorbent assay (ULISA).2 Such labels can enhance the assay sensitivity significantly. However, as many biomarkers are extremely low-abundant in body fluids, even ULISA may still not be sensitive enough such that new, even more sensitive assay schemes are needed. Magnetic microparticles (MBs) are a promising alternative solid phase for microtiter plate immunoassays. The superparamagnetic properties of MBs allow for analyte preconcentration and thus improve the assay sensitivity.3 We have developed and optimized a sandwich immunoassay for the detection of PSA based on MBs and UCNP-based labels. We conjugated one type of monoclonal anti-PSA antibody to MBs and the other one to UCNPs via streptavidin-biotin binding, and tested their functionality. Optimal concentrations of MBs and serum were selected, followed by the fully optimized analysis of model real samples of spiked serum. The assay reached an LOD of 11.1 pg/mL, comparable to an ELISA using identical immunoreagents. An additional magnetic preconcentration step further improved the LOD to 0.25 pg/mL, which was 3-fold better compared to the ELISA, and 44-fold better than an MB-based ELISA using identical immunoreagents. These results demonstrate the potential of using MBs combined with UCNPs for the sensitive detection of cancer biomarkers.

Návaznosti

MUNI/A/1313/2022, interní kód MU

Název: Podpora biochemického výzkumu v roce 2023

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2023

Citovat

SKLENÁROVÁ, Dorota, Ekaterina MAKHNEVA, Julian BRANDMEIER, Hans-Heiner GORRIS a Zdeněk FARKA. Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles. In 23rd Joint meeting of Biophysical Chemists and Electrochemists 2023. 2023.

@proceedings{2365902,
   author = {Sklenárová, Dorota and Makhneva, Ekaterina and Brandmeier, Julian and Gorris, HansandHeiner and Farka, Zdeněk},
   booktitle = {23rd Joint meeting of Biophysical Chemists and Electrochemists 2023},
   keywords = {protein biomarkers, prostate cancer, immunoassays, magnetic microparticles, upconversion nanoparticles},
   title = {Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles},
   url = {https://www.biophys-electrochem.cz/},
   year = {2023}
}

TY  - CONF
ID  - 2365902
AU  - Sklenárová, Dorota - Makhneva, Ekaterina - Brandmeier, Julian - Gorris, Hans-Heiner - Farka, Zdeněk
PY  - 2023
TI  - Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles
KW  - protein biomarkers, prostate cancer, immunoassays, magnetic microparticles, upconversion nanoparticles
UR  - https://www.biophys-electrochem.cz/
N2  - Prostate cancer is the leading cause of death within the male population worldwide caused by oncologic diseases. The glycoprotein prostate-specific antigen (PSA) produced by the prostate is its most important biomarker. While blood serum concentrations up to 2.5 ng/mL are considered normal and can be generally detected by conventional immunoassays, more sensitive detection schemes are essential for early-stage disease diagnosis. Immunochemical methods, such as the enzyme-linked immunosorbent assay (ELISA), are considered the gold standard for PSA detection. However, there are several limitations that render ELISA insufficient regarding the sensitivity required for early-stage diagnosis.1 Photon-upconversion nanoparticles (UCNPs) are luminescent nanocrystals consisting of an inorganic matrix doped with lanthanide ions. They exhibit anti-Stokes luminescence (the ability to convert low-energy excitation to higher energy emission), thus avoiding optical background interference. When conjugated with biorecognition molecules (e.g., antibodies or streptavidin), UCNPs can be used as a sensitive label in various immunoassay formats, including microtiter plate-based upconversion-linked immunosorbent assay (ULISA).2 Such labels can enhance the assay sensitivity significantly. However, as many biomarkers are extremely low-abundant in body fluids, even ULISA may still not be sensitive enough such that new, even more sensitive assay schemes are needed. Magnetic microparticles (MBs) are a promising alternative solid phase for microtiter plate immunoassays. The superparamagnetic properties of MBs allow for analyte preconcentration and thus improve the assay sensitivity.3 We have developed and optimized a sandwich immunoassay for the detection of PSA based on MBs and UCNP-based labels. We conjugated one type of monoclonal anti-PSA antibody to MBs and the other one to UCNPs via streptavidin-biotin binding, and tested their functionality. Optimal concentrations of MBs and serum were selected, followed by the fully optimized analysis of model real samples of spiked serum. The assay reached an LOD of 11.1 pg/mL, comparable to an ELISA using identical immunoreagents. An additional magnetic preconcentration step further improved the LOD to 0.25 pg/mL, which was 3-fold better compared to the ELISA, and 44-fold better than an MB-based ELISA using identical immunoreagents. These results demonstrate the potential of using MBs combined with UCNPs for the sensitive detection of cancer biomarkers.
ER  -

SKLENÁROVÁ, Dorota, Ekaterina MAKHNEVA, Julian BRANDMEIER, Hans-Heiner GORRIS a Zdeněk FARKA. Detection of prostate-specific antigen utilizing immunomagnetic assay with upconversion nanoparticles. In \textit{23rd Joint meeting of Biophysical Chemists and Electrochemists 2023}. 2023.

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