LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ, Oriana LO RE, Irena KOUTNÁ, Tommaso MAZZA a Manlio VINCIGUERRA. Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming. Epigenomics. Future Medicine Ltd, 2023, roč. 15, č. 17, s. 863-877. ISSN 1750-1911. Dostupné z: https://dx.doi.org/10.2217/epi-2023-0267. |
Další formáty:
BibTeX
LaTeX
RIS
@article{2367478, author = {Liorni, Niccolo and Napoli, Alessandro and Castellana, Stefano and Giallongo, Sebastiano and Hulínová, Daniela and Lo Re, Oriana and Koutná, Irena and Mazza, Tommaso and Vinciguerra, Manlio}, article_number = {17}, doi = {http://dx.doi.org/10.2217/epi-2023-0267}, keywords = {CUT&Tag; induced pluripotent stem cells; iPSCs; reprogramming; somatic cells}, language = {eng}, issn = {1750-1911}, journal = {Epigenomics}, title = {Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming}, url = {https://www.futuremedicine.com/doi/10.2217/epi-2023-0267}, volume = {15}, year = {2023} }
TY - JOUR ID - 2367478 AU - Liorni, Niccolo - Napoli, Alessandro - Castellana, Stefano - Giallongo, Sebastiano - Hulínová, Daniela - Lo Re, Oriana - Koutná, Irena - Mazza, Tommaso - Vinciguerra, Manlio PY - 2023 TI - Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming JF - Epigenomics VL - 15 IS - 17 SP - 863-877 EP - 863-877 PB - Future Medicine Ltd SN - 17501911 KW - CUT&Tag KW - induced pluripotent stem cells KW - iPSCs KW - reprogramming KW - somatic cells UR - https://www.futuremedicine.com/doi/10.2217/epi-2023-0267 N2 - Aim:Human induced pluripotent stem cells (iPSCs) are inefficiently derived from somatic cells by overexpression of defined transcription factors. Overexpression of H2A histone variant macroH2A1.1, but not macroH2A1.2, leads to increased iPSC reprogramming by unclear mechanisms. Materials & methods:Cleavage under targets and tagmentation (CUT&Tag) allows robust epigenomic profiling of a low cell number. We performed an integrative CUT&Tag-RNA-Seq analysis of macroH2A1-dependent orchestration of iPSCs reprogramming using human endothelial cells. Results:We demonstrate wider genome occupancy, predicted transcription factors binding, and gene expression regulated by macroH2A1.1 during reprogramming, compared to macroH2A1.2. MacroH2A1.1, previously associated with neurodegenerative pathologies, specifically activated ectoderm/neural processes. Conclusion:CUT&Tag and RNA-Seq data integration is a powerful tool to investigate the epigenetic mechanisms occurring during cell reprogramming. ER -
LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ, Oriana LO RE, Irena KOUTNÁ, Tommaso MAZZA a Manlio VINCIGUERRA. Integrative CUT\&{}Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming. \textit{Epigenomics}. Future Medicine Ltd, 2023, roč.~15, č.~17, s.~863-877. ISSN~1750-1911. Dostupné z: https://dx.doi.org/10.2217/epi-2023-0267.
|