Integrative CUT&Tag-RNA-Seq analysis of histone variant
macroH2A1-dependent orchestration of human induced pluripotent
stem cell reprogramming

J 2023

Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming

LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ et. al.

Základní údaje

Originální název

Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming

Autoři

LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ (203 Česká republika, domácí), Oriana LO RE, Irena KOUTNÁ (203 Česká republika, domácí), Tommaso MAZZA a Manlio VINCIGUERRA

Vydání

Epigenomics, Future Medicine Ltd, 2023, 1750-1911

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.800 v roce 2022

Kód RIV

RIV/00216224:14110/23:00133308

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2217/epi-2023-0267

UT WoS

001085184900001

Klíčová slova anglicky

CUT&Tag; induced pluripotent stem cells; iPSCs; reprogramming; somatic cells

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 15:58, Mgr. Marie Šípková, DiS.

Anotace

V originále

Aim:Human induced pluripotent stem cells (iPSCs) are inefficiently derived from somatic cells by overexpression of defined transcription factors. Overexpression of H2A histone variant macroH2A1.1, but not macroH2A1.2, leads to increased iPSC reprogramming by unclear mechanisms. Materials & methods:Cleavage under targets and tagmentation (CUT&Tag) allows robust epigenomic profiling of a low cell number. We performed an integrative CUT&Tag-RNA-Seq analysis of macroH2A1-dependent orchestration of iPSCs reprogramming using human endothelial cells. Results:We demonstrate wider genome occupancy, predicted transcription factors binding, and gene expression regulated by macroH2A1.1 during reprogramming, compared to macroH2A1.2. MacroH2A1.1, previously associated with neurodegenerative pathologies, specifically activated ectoderm/neural processes. Conclusion:CUT&Tag and RNA-Seq data integration is a powerful tool to investigate the epigenetic mechanisms occurring during cell reprogramming.

Citovat

LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ, Oriana LO RE, Irena KOUTNÁ, Tommaso MAZZA a Manlio VINCIGUERRA. Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming. Epigenomics. Future Medicine Ltd, 2023, roč. 15, č. 17, s. 863-877. ISSN 1750-1911. Dostupné z: https://dx.doi.org/10.2217/epi-2023-0267.

@article{2367478,
   author = {Liorni, Niccolo and Napoli, Alessandro and Castellana, Stefano and Giallongo, Sebastiano and Hulínová, Daniela and Lo Re, Oriana and Koutná, Irena and Mazza, Tommaso and Vinciguerra, Manlio},
   article_number = {17},
   doi = {http://dx.doi.org/10.2217/epi-2023-0267},
   keywords = {CUT&Tag; induced pluripotent stem cells; iPSCs; reprogramming; somatic cells},
   language = {eng},
   issn = {1750-1911},
   journal = {Epigenomics},
   title = {Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming},
   url = {https://www.futuremedicine.com/doi/10.2217/epi-2023-0267},
   volume = {15},
   year = {2023}
}

TY  - JOUR
ID  - 2367478
AU  - Liorni, Niccolo - Napoli, Alessandro - Castellana, Stefano - Giallongo, Sebastiano - Hulínová, Daniela - Lo Re, Oriana - Koutná, Irena - Mazza, Tommaso - Vinciguerra, Manlio
PY  - 2023
TI  - Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming
JF  - Epigenomics
VL  - 15
IS  - 17
SP  - 863-877
EP  - 863-877
PB  - Future Medicine Ltd
SN  - 17501911
KW  - CUT&Tag
KW  - induced pluripotent stem cells
KW  - iPSCs
KW  - reprogramming
KW  - somatic cells
UR  - https://www.futuremedicine.com/doi/10.2217/epi-2023-0267
N2  - Aim:Human induced pluripotent stem cells (iPSCs) are inefficiently derived from somatic cells by overexpression of defined transcription factors. Overexpression of H2A histone variant macroH2A1.1, but not macroH2A1.2, leads to increased iPSC reprogramming by unclear mechanisms. Materials & methods:Cleavage under targets and tagmentation (CUT&Tag) allows robust epigenomic profiling of a low cell number. We performed an integrative CUT&Tag-RNA-Seq analysis of macroH2A1-dependent orchestration of iPSCs reprogramming using human endothelial cells. Results:We demonstrate wider genome occupancy, predicted transcription factors binding, and gene expression regulated by macroH2A1.1 during reprogramming, compared to macroH2A1.2. MacroH2A1.1, previously associated with neurodegenerative pathologies, specifically activated ectoderm/neural processes. Conclusion:CUT&Tag and RNA-Seq data integration is a powerful tool to investigate the epigenetic mechanisms occurring during cell reprogramming.
ER  -

LIORNI, Niccolo, Alessandro NAPOLI, Stefano CASTELLANA, Sebastiano GIALLONGO, Daniela HULÍNOVÁ, Oriana LO RE, Irena KOUTNÁ, Tommaso MAZZA a Manlio VINCIGUERRA. Integrative CUT\&{}Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming. \textit{Epigenomics}. Future Medicine Ltd, 2023, roč.~15, č.~17, s.~863-877. ISSN~1750-1911. Dostupné z: https://dx.doi.org/10.2217/epi-2023-0267.

Podrobný výpis o publikaci