EVIC, Valentina, Ruzica SOIC, Marko MOCIBOB, Mario KEKEZ, Josef HOUSER, Michaela WIMMEROVÁ, Dubravka MATKOVIĆ-ČALOGOVIĆ, Ita GRUIC-SOVULJ, Ivana KEKEZ a Jasmina ROKOV-PLAVEC. Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation. FEBS Letters. Amsterdam: Elsevier Science, 2023, roč. 597, č. 23, s. 2975-2992. ISSN 0014-5793. Dostupné z: https://dx.doi.org/10.1002/1873-3468.14748. |
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@article{2367499, author = {Evic, Valentina and Soic, Ruzica and Mocibob, Marko and Kekez, Mario and Houser, Josef and Wimmerová, Michaela and MatkovićandČalogović, Dubravka and GruicandSovulj, Ita and Kekez, Ivana and RokovandPlavec, Jasmina}, article_location = {Amsterdam}, article_number = {23}, doi = {http://dx.doi.org/10.1002/1873-3468.14748}, keywords = {aminoacyl-tRNA synthetase; cysteine reactivity; disulfide bond; hydrogen peroxide; oxidative stress; thermal stability}, language = {eng}, issn = {0014-5793}, journal = {FEBS Letters}, title = {Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation}, url = {https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14748}, volume = {597}, year = {2023} }
TY - JOUR ID - 2367499 AU - Evic, Valentina - Soic, Ruzica - Mocibob, Marko - Kekez, Mario - Houser, Josef - Wimmerová, Michaela - Matković-Čalogović, Dubravka - Gruic-Sovulj, Ita - Kekez, Ivana - Rokov-Plavec, Jasmina PY - 2023 TI - Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation JF - FEBS Letters VL - 597 IS - 23 SP - 2975-2992 EP - 2975-2992 PB - Elsevier Science SN - 00145793 KW - aminoacyl-tRNA synthetase KW - cysteine reactivity KW - disulfide bond KW - hydrogen peroxide KW - oxidative stress KW - thermal stability UR - https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14748 N2 - We have previously identified a unique disulfide bond in the crystal structure of Arabidopsis cytosolic seryl-tRNA synthetase involving cysteines evolutionarily conserved in all green plants. Here, we discovered that both cysteines are important for protein stability, but with opposite effects, and that their microenvironment may promote disulfide bond formation in oxidizing conditions. The crystal structure of the C244S mutant exhibited higher rigidity and an extensive network of noncovalent interactions correlating with its higher thermal stability. The activity of the wild-type showed resistance to oxidation with H2O2, while the activities of cysteine-to-serine mutants were impaired, indicating that the disulfide link may enable the protein to function under oxidative stress conditions which can be beneficial for an efficient plant stress response. ER -
EVIC, Valentina, Ruzica SOIC, Marko MOCIBOB, Mario KEKEZ, Josef HOUSER, Michaela WIMMEROVÁ, Dubravka MATKOVI$\backslash$'C-ČALOGOVI$\backslash$'C, Ita GRUIC-SOVULJ, Ivana KEKEZ a Jasmina ROKOV-PLAVEC. Evolutionarily conserved cysteines in plant cytosolic seryl-tRNA synthetase are important for its resistance to oxidation. \textit{FEBS Letters}. Amsterdam: Elsevier Science, 2023, roč.~597, č.~23, s.~2975-2992. ISSN~0014-5793. Dostupné z: https://dx.doi.org/10.1002/1873-3468.14748.
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