Exploring new galaxies: Perspectives on the discovery of novel
PET-degrading enzymes

J 2024

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO et. al.

Základní údaje

Originální název

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

Autoři

MIČAN, Jan (203 Česká republika, domácí), Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO (643 Rusko, domácí), Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ (203 Česká republika, garant, domácí), Ren WEI a David BEDNÁŘ (203 Česká republika, domácí)

Vydání

Applied Catalysis B: Environment and Energy, Amsterdam, Elsevier, 2024, 0926-3373

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 22.100 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.apcatb.2023.123404

UT WoS

001110359200001

Klíčová slova anglicky

PET recycling; Protein engineering; Enzymatic degradation; Microbial dark matter; AlphaFold; High -throughput screening

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 10:36, Mgr. Marie Šípková, DiS.

Anotace

V originále

Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

FW03010208, projekt VaV

Název: Stabilizace aplikačně atraktivních FGF proteinů pomocí pokročilých automatizovaných metod proteinového inženýrství

Investor: Technologická agentura ČR, Stabilizace aplikačně atraktivních FGF proteinů pomocí pokročilých metod proteinového inženýrství, Podprogram 1 "Technologičtí lídři"

LM2023055, projekt VaV

Název: Česká národní infrastruktura pro biologická data

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, ELIXIR-CZ: Česká národní infrastruktura pro biologická data

LM2023069, projekt VaV

Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO, Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ, Ren WEI a David BEDNÁŘ. Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes. Applied Catalysis B: Environment and Energy. Amsterdam: Elsevier, 2024, roč. 342, March 2024, s. 1-16. ISSN 0926-3373. Dostupné z: https://dx.doi.org/10.1016/j.apcatb.2023.123404.

@article{2367868,
   author = {Mičan, Jan and Jaradat, Da 'san M. M. and Liu, Weidong and Weber, Gert and Mazurenko, Stanislav and Bornscheuer, Uwe T. and Damborský, Jiří and Wei, Ren and Bednář, David},
   article_location = {Amsterdam},
   article_number = {March 2024},
   doi = {http://dx.doi.org/10.1016/j.apcatb.2023.123404},
   keywords = {PET recycling; Protein engineering; Enzymatic degradation; Microbial dark matter; AlphaFold; High -throughput screening},
   language = {eng},
   issn = {0926-3373},
   journal = {Applied Catalysis B: Environment and Energy},
   note = {LF žádný přínos dle informací od doc. Bednáře.},
   title = {Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes},
   url = {https://www.sciencedirect.com/science/article/pii/S0926337323010470?via%3Dihub},
   volume = {342},
   year = {2024}
}

TY  - JOUR
ID  - 2367868
AU  - Mičan, Jan - Jaradat, Da 'san M. M. - Liu, Weidong - Weber, Gert - Mazurenko, Stanislav - Bornscheuer, Uwe T. - Damborský, Jiří - Wei, Ren - Bednář, David
PY  - 2024
TI  - Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes
JF  - Applied Catalysis B: Environment and Energy
VL  - 342
IS  - March 2024
SP  - 1-16
EP  - 1-16
PB  - Elsevier
SN  - 09263373
N1  - LF žádný přínos dle informací od doc. Bednáře.
KW  - PET recycling
KW  - Protein engineering
KW  - Enzymatic degradation
KW  - Microbial dark matter
KW  - AlphaFold
KW  - High -throughput screening
UR  - https://www.sciencedirect.com/science/article/pii/S0926337323010470?via%3Dihub
N2  - Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.
ER  -

MIČAN, Jan, Da 'san M. M. JARADAT, Weidong LIU, Gert WEBER, Stanislav MAZURENKO, Uwe T. BORNSCHEUER, Jiří DAMBORSKÝ, Ren WEI a David BEDNÁŘ. Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes. \textit{Applied Catalysis B: Environment and Energy}. Amsterdam: Elsevier, 2024, roč.~342, March 2024, s.~1-16. ISSN~0926-3373. Dostupné z: https://dx.doi.org/10.1016/j.apcatb.2023.123404.

Podrobný výpis o publikaci