Listeria monocytogenes clones circulating in the natural
environment of the Czech Republic and Slovakia

J 2023

Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ a Renata KARPÍŠKOVÁ

Základní údaje

Originální název

Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia

Autoři

TOMASTIKOVA, Zuzana (203 Česká republika), Lucie HLUCHANOVA (203 Česká republika, garant), Tereza GELBÍČOVÁ (203 Česká republika, domácí) a Renata KARPÍŠKOVÁ (203 Česká republika, domácí)

Vydání

Czech Journal of Food Sciences, Praha, ČR, Czech Academy of Agricultural Sciences, 2023, 1212-1800

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.300 v roce 2022

Kód RIV

RIV/00216224:14110/23:00133381

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.17221/234/2022-CJFS

UT WoS

000974395300001

Klíčová slova anglicky

nature; serotyping; macrorestriction analysis; antimicrobial susceptibility; whole genome sequencing

Štítky

14110525, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 2. 2024 12:38, Mgr. Tereza Miškechová

Anotace

V originále

Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016-2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.

Citovat

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ a Renata KARPÍŠKOVÁ. Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia. Czech Journal of Food Sciences. Praha, ČR: Czech Academy of Agricultural Sciences, 2023, roč. 41, č. 2, s. 127-136. ISSN 1212-1800. Dostupné z: https://dx.doi.org/10.17221/234/2022-CJFS.

@article{2368628,
   author = {Tomastikova, Zuzana and Hluchanova, Lucie and Gelbíčová, Tereza and Karpíšková, Renata},
   article_location = {Praha, ČR},
   article_number = {2},
   doi = {http://dx.doi.org/10.17221/234/2022-CJFS},
   keywords = {nature; serotyping; macrorestriction analysis; antimicrobial susceptibility; whole genome sequencing},
   language = {eng},
   issn = {1212-1800},
   journal = {Czech Journal of Food Sciences},
   title = {Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia},
   url = {https://cjfs.agriculturejournals.cz/artkey/cjf-202302-0006_listeria-monocytogenes-clones-circulating-in-the-natural-environment-of-the-czech-republic-and-slovakia.php},
   volume = {41},
   year = {2023}
}

TY  - JOUR
ID  - 2368628
AU  - Tomastikova, Zuzana - Hluchanova, Lucie - Gelbíčová, Tereza - Karpíšková, Renata
PY  - 2023
TI  - Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia
JF  - Czech Journal of Food Sciences
VL  - 41
IS  - 2
SP  - 127-136
EP  - 127-136
PB  - Czech Academy of Agricultural Sciences
SN  - 12121800
KW  - nature
KW  - serotyping
KW  - macrorestriction analysis
KW  - antimicrobial susceptibility
KW  - whole genome sequencing
UR  - https://cjfs.agriculturejournals.cz/artkey/cjf-202302-0006_listeria-monocytogenes-clones-circulating-in-the-natural-environment-of-the-czech-republic-and-slovakia.php
N2  - Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016-2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.
ER  -

TOMASTIKOVA, Zuzana, Lucie HLUCHANOVA, Tereza GELBÍČOVÁ a Renata KARPÍŠKOVÁ. Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia. \textit{Czech Journal of Food Sciences}. Praha, ČR: Czech Academy of Agricultural Sciences, 2023, roč.~41, č.~2, s.~127-136. ISSN~1212-1800. Dostupné z: https://dx.doi.org/10.17221/234/2022-CJFS.

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