RIHACEK, Martin, Michaela KUTHANOVA, Zbynek SPLICHAL, Vojtech ADAM, Kristyna HRAZDILOVA, Radek VESELÝ, Ludek ZUREK a Kristyna CIHALOVA. Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps. INFECTION AND DRUG RESISTANCE. AUCKLAND: DOVE MEDICAL PRESS LTD, 2023, roč. 16, November 2023, s. 7365-7375. ISSN 1178-6973. Dostupné z: https://dx.doi.org/10.2147/IDR.S435622.
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Základní údaje
Originální název Escherichia coli from Human Wounds: Analysis of Resistance to β-Lactams and Expression of RND Efflux Pumps
Autoři RIHACEK, Martin (203 Česká republika), Michaela KUTHANOVA (203 Česká republika), Zbynek SPLICHAL (203 Česká republika), Vojtech ADAM (203 Česká republika), Kristyna HRAZDILOVA (203 Česká republika), Radek VESELÝ (203 Česká republika, domácí), Ludek ZUREK (203 Česká republika) a Kristyna CIHALOVA (203 Česká republika, garant).
Vydání INFECTION AND DRUG RESISTANCE, AUCKLAND, DOVE MEDICAL PRESS LTD, 2023, 1178-6973.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30230 Other clinical medicine subjects
Stát vydavatele Nový Zéland
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 3.900 v roce 2022
Kód RIV RIV/00216224:14110/23:00133383
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.2147/IDR.S435622
UT WoS 001113170700001
Klíčová slova anglicky clinical isolates; ampicillin resistance; resistance mechanisms; antibiotics
Štítky 14110711, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 19. 2. 2024 15:25.
Anotace
Purpose: Resistance of pathogenic strains of Escherichia coli to beta-lactams, particularly to ampicillin, is on the rise and it is attributed to intrinsic and acquired mechanisms. One important factor contributing to resistance, together with primarily resistance mechanisms, is a mutation and/or an over-expression of the intrinsic efflux pumps in the resistance-nodulation-division (RND) superfamily. Among these efflux pumps, AcrA, AcrB, TolC, and AcrD play an important role in antimicrobial co-resistance, including resistance to beta-lactams.Materials and Methods: Twelve E. coli isolates obtained from patients' wounds and the control strain of E. coli ATCC 25922 were analyzed. The phenotypic resistance of these isolates to selected beta-lactams was assessed by determination of the minimal inhibitory concentration. Additionally, the prevalence of beta-lactamase genes (blaTEM, blaCTX-M, blaSHV, and blaAmpC) was screened by PCR. Realtime qPCR was used to determine the expression of the selected efflux pumps acrA, acrB, tolC, and acrD and the repressor acrR after the exposure of E. coli to ampicillin.Results: Phenotypic resistance to beta-lactams was detected in seven isolates, mainly to ampicillin and piperacillin. This was corroborated by the presence of at least one acquired bla gene in each of these isolates. Although E. coli strains varied in the expression of RND-family efflux pumps after the ampicillin exposure, their gene expression indicated that these pumps did not play a major role in the phenotypic resistance to ampicillin.Conclusion: Each E. coli isolate displayed unique characteristics, differing in minimum inhibitory concentration (MIC) values, prevalence of acquired blaTEM and blaCTX-M genes, and expression of the RND-family pumps. This together demonstrates that these clinical isolates employed distinct intrinsic or acquired resistance pathways for their defense against ampicillin. The prevalence and spread of ampicillin resistant E. coli has to be monitored and the search for ampicillin alternatives is needed.
VytisknoutZobrazeno: 9. 7. 2024 12:17