Preparative agarose gel electrophoresis for reducing matrix
interferences of organoid cell medium prior to LC-MS analysis
of insulin

J 2024

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT et. al.

Základní údaje

Originální název

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Autoři

HRUŠKOVÁ, Helena (203 Česká republika, domácí), Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET a Steven Ray WILSON

Vydání

Journal of Chromatography A, Elsevier B.V. 2024, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2024.464669

UT WoS

001173156700001

Klíčová slova anglicky

Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2024 12:07, Mgr. Marie Šípková, DiS.

Anotace

V originále

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.

Citovat

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET a Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. Journal of Chromatography A. Elsevier B.V., 2024, roč. 1717, February, s. 1-7. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2024.464669.

@article{2369020,
   author = {Hrušková, Helena and Olsen, Christine and Řemínek, Roman and Wang, Chencheng and Aizenshtadt, Aleksandra and Krauss, Stefan and Scholz, Hanne and RøbergandLarsen, Hanne and Foret, František and Wilson, Steven Ray},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.chroma.2024.464669},
   keywords = {Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967324000426},
   volume = {1717},
   year = {2024}
}

TY  - JOUR
ID  - 2369020
AU  - Hrušková, Helena - Olsen, Christine - Řemínek, Roman - Wang, Chencheng - Aizenshtadt, Aleksandra - Krauss, Stefan - Scholz, Hanne - Røberg-Larsen, Hanne - Foret, František - Wilson, Steven Ray
PY  - 2024
TI  - Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
JF  - Journal of Chromatography A
VL  - 1717
IS  - February
SP  - 1-7
EP  - 1-7
PB  - Elsevier B.V.
SN  - 00219673
KW  - Liquid chromatography-mass spectrometry
KW  - Insulin
KW  - Preparative gel electrophoresis
KW  - Stem cell-derived islet organoids
UR  - https://www.sciencedirect.com/science/article/pii/S0021967324000426
N2  - Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.
ER  -

HRUŠKOVÁ, Helena, Christine OLSEN, Roman ŘEMÍNEK, Chencheng WANG, Aleksandra AIZENSHTADT, Stefan KRAUSS, Hanne SCHOLZ, Hanne RØBERG-LARSEN, František FORET a Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. \textit{Journal of Chromatography A}. Elsevier B.V., 2024, roč.~1717, February, s.~1-7. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2024.464669.

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