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@article{2370317,
author = {Silveri, Filippo and Obořilová, Radka and Máčala, Jakub and Compagnone, Dario and Skládal, Petr},
article_location = {Wien},
article_number = {8},
doi = {http://dx.doi.org/10.1007/s00604-023-05873-1},
keywords = {Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion},
language = {eng},
issn = {0026-3672},
journal = {Microchimica Acta},
title = {Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite},
url = {https://link.springer.com/article/10.1007/s00604-023-05873-1},
volume = {190},
year = {2023}
}
TY - JOUR
ID - 2370317
AU - Silveri, Filippo - Obořilová, Radka - Máčala, Jakub - Compagnone, Dario - Skládal, Petr
PY - 2023
TI - Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
JF - Microchimica Acta
VL - 190
IS - 8
SP - 1-12
EP - 1-12
PB - Springer
SN - 00263672
KW - Human serum albumin
KW - Impedimetric sensor
KW - Transition metal dichalcogenides
KW - Nanocomposite
KW - Aqueous-based dispersion
UR - https://link.springer.com/article/10.1007/s00604-023-05873-1
N2 - An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.
ER -
SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE a Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. \textit{Microchimica Acta}. Wien: Springer, 2023, roč.~190, č.~8, s.~1-12. ISSN~0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-023-05873-1.
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