An open source 3D printed autosampler for capillary
electrophoresis

J 2023

An open source 3D printed autosampler for capillary electrophoresis

ITTERHEIMOVÁ, Petra a Petr KUBAN

Základní údaje

Originální název

An open source 3D printed autosampler for capillary electrophoresis

Autoři

ITTERHEIMOVÁ, Petra (203 Česká republika, domácí) a Petr KUBAN (garant)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2023, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2022

Kód RIV

RIV/00216224:14740/23:00133474

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.aca.2023.341832

UT WoS

001084087000001

Klíčová slova anglicky

Capillary electrophoresis; Autosampler; Open source hardware and software; Arduino

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 3. 2024 15:11, Mgr. Eva Dubská

Anotace

V originále

Background: In-house built capillary electrophoresis (CE) systems represent a significant share of laboratory instrumentation. In most of these instruments, sample injection is effected manually with low to moderate precision and requires skilled operators. Although few automated samplers have been previously developed, typically only one sample at a time can be injected. If a series of samples is to be analyzed, manual intervention is required. In the present work, we developed and constructed a fully automated, open source, CE autosampler, able to handle up to 14 different samples that can be used as a modular component of any in-house built CE instrument. Results: An inexpensive, 3D printed, open source, autosampler for CE was developed. The autosampler consists of two parts: an injection unit with carousel containing sample and electrolyte vials and a flushing unit, containing a miniature pressure/vacuum pump. The autosampler is operated by an Arduino Mega microcontroller and an Arduino code written in the laboratory. The injection sequence is entered through a keypad and LCD display by the user. The instrument can operate autonomously for extended periods of time. It was used for fully automated analysis and/or calibration of up to 14 samples with excellent injection repeatability reaching less than 2.7% RSD for peak areas. The sampler performance was tested with two independently built CE instruments, a CE system with contactless conductivity detection (C4D) and a CE system with laser induced fluorescence (LIF) detector.Significance and novelty: A novel, 3D printed, Arduino-based autosampler for CE was developed. The autosampler allows autonomous hydrodynamic injection of up to 14 different samples with fully programmable injection sequence, including capillary flushing and high voltage and data acquisition control. It provides the missing instrumental sampling setup for laboratory made CE instruments. It can be simply constructed based on the open -source blueprints in any laboratory and be a useful and time-saving add-on to any modular CE instrument.

Citovat

ITTERHEIMOVÁ, Petra a Petr KUBAN. An open source 3D printed autosampler for capillary electrophoresis. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2023, roč. 1279, oct, s. 1-8. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341832.

@article{2370877,
   author = {Itterheimová, Petra and Kuban, Petr},
   article_location = {Amsterdam},
   article_number = {oct},
   doi = {http://dx.doi.org/10.1016/j.aca.2023.341832},
   keywords = {Capillary electrophoresis; Autosampler; Open source hardware and software; Arduino},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {An open source 3D printed autosampler for capillary electrophoresis},
   url = {https://www.sciencedirect.com/science/article/pii/S000326702301053X?via%3Dihub},
   volume = {1279},
   year = {2023}
}

TY  - JOUR
ID  - 2370877
AU  - Itterheimová, Petra - Kuban, Petr
PY  - 2023
TI  - An open source 3D printed autosampler for capillary electrophoresis
JF  - Analytica Chimica Acta
VL  - 1279
IS  - oct
SP  - 1-8
EP  - 1-8
PB  - Elsevier Science publishers
SN  - 00032670
KW  - Capillary electrophoresis
KW  - Autosampler
KW  - Open source hardware and software
KW  - Arduino
UR  - https://www.sciencedirect.com/science/article/pii/S000326702301053X?via%3Dihub
N2  - Background: In-house built capillary electrophoresis (CE) systems represent a significant share of laboratory instrumentation. In most of these instruments, sample injection is effected manually with low to moderate precision and requires skilled operators. Although few automated samplers have been previously developed, typically only one sample at a time can be injected. If a series of samples is to be analyzed, manual intervention is required. In the present work, we developed and constructed a fully automated, open source, CE autosampler, able to handle up to 14 different samples that can be used as a modular component of any in-house built CE instrument. Results: An inexpensive, 3D printed, open source, autosampler for CE was developed. The autosampler consists of two parts: an injection unit with carousel containing sample and electrolyte vials and a flushing unit, containing a miniature pressure/vacuum pump. The autosampler is operated by an Arduino Mega microcontroller and an Arduino code written in the laboratory. The injection sequence is entered through a keypad and LCD display by the user. The instrument can operate autonomously for extended periods of time. It was used for fully automated analysis and/or calibration of up to 14 samples with excellent injection repeatability reaching less than 2.7% RSD for peak areas. The sampler performance was tested with two independently built CE instruments, a CE system with contactless conductivity detection (C4D) and a CE system with laser induced fluorescence (LIF) detector.Significance and novelty: A novel, 3D printed, Arduino-based autosampler for CE was developed. The autosampler allows autonomous hydrodynamic injection of up to 14 different samples with fully programmable injection sequence, including capillary flushing and high voltage and data acquisition control. It provides the missing instrumental sampling setup for laboratory made CE instruments. It can be simply constructed based on the open -source blueprints in any laboratory and be a useful and time-saving add-on to any modular CE instrument.
ER  -

ITTERHEIMOVÁ, Petra a Petr KUBAN. An open source 3D printed autosampler for capillary electrophoresis. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2023, roč.~1279, oct, s.~1-8. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2023.341832.

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