D 2021

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

RUTTKAY-NEDECKÝ, Branislav, Karel SEHNAL, Dominik BANÁŠ, Martina STANKOVA, Marta KEPINSKA et. al.

Základní údaje

Originální název

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

Autoři

RUTTKAY-NEDECKÝ, Branislav (203 Česká republika, domácí), Karel SEHNAL (203 Česká republika, domácí), Dominik BANÁŠ (703 Slovensko, domácí), Martina STANKOVA, Marta KEPINSKA, Duong Ngonc BACH, Jiri SOCHOR, Mojmir BARON, Awais FARID, Hoai Viet NGUYEN a René KIZEK (203 Česká republika, domácí)

Vydání

SLEZSKA, 12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020), od s. 422-427, 6 s. 2021

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

Kód RIV

RIV/00216224:14160/21:00133494

Organizační jednotka

Farmaceutická fakulta

ISBN

978-80-87294-98-7

ISSN

UT WoS

000664505500072

Klíčová slova anglicky

Green synthesis; phenolic compounds; analytical methods; antibacterial effects; traditional medicine; ethnobotany

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 9. 2. 2024 09:04, Mgr. Marie Šípková, DiS.

Anotace

V originále

Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.