BEZDEKOVA, Jaroslava, Mariana PLEVOVÁ, Lukas NEJDL, Mirek MACKA, Michal MASAŘÍK, Dalibor PACIK, Vojtech ADAM a Marketa VACULOVICOVA. Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device. Sensors and Actuators B: Chemical. LAUSANNE: Elsevier B.V., 2024, roč. 403, March 2024, s. 1-10. ISSN 0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2023.135146.
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Základní údaje
Originální název Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device
Autoři BEZDEKOVA, Jaroslava (203 Česká republika), Mariana PLEVOVÁ (703 Slovensko), Lukas NEJDL (203 Česká republika), Mirek MACKA (203 Česká republika), Michal MASAŘÍK (203 Česká republika, domácí), Dalibor PACIK (203 Česká republika), Vojtech ADAM (203 Česká republika) a Marketa VACULOVICOVA (203 Česká republika, garant).
Vydání Sensors and Actuators B: Chemical, LAUSANNE, Elsevier B.V. 2024, 0925-4005.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30204 Oncology
Stát vydavatele Švýcarsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 8.400 v roce 2022
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1016/j.snb.2023.135146
UT WoS 001143020700001
Klíčová slova anglicky Molecularly imprinted polymers; UV -induced fluorescent clusters; UV -induced fluorescence fingerprinting
Štítky 14110518, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 23. 2. 2024 12:33.
Anotace
The early detection of prostate cancer (CaP), one of the most common cancers in males, improves treatment efficacy. However, current methods to detect CaP are limited by specificity and sensitivity or require an invasive and unpleasant procedure. Therefore, there is a need for a novel, fast, and noninvasive CaP detection method. This proof-of-concept study aimed to diagnose CaP from urine samples by detecting the UV-induced fluorescent clusters formed in situ that reflect a urine composition specific to CaP patients using fluorescence spectroscopy. The UV-induced clusters formed upon irradiation by UV light with a wavelength of 254 nm were detected at excitation and emission wavelengths of 400 nm and 460 nm. Fifteen patients with CaP were correctly distinguished from 5 controls. The different stages of CaP could be further determined using molecular imprinting technology. Additionally, a paper-based analytical device was developed that enabled the correct identification of stage I cancer patients by fluorescent spectroscopy detection and the naked eye. In conclusion, this novel, easyto-operate, point-of-care assay can enable early diagnosis of CaP with high accuracy and judgement of the disease stage. The simplicity, as well as versatility of the proposed method, will enable its application also to other diseases and disorders.
VytisknoutZobrazeno: 3. 7. 2024 03:11