Diversity of RNA viruses in the cosmopolitan monoxenous
trypanosomatid Leptomonas pyrrhocoris

J 2023

Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

MACEDO, Diego H, Danyil GRYBCHUK, Jana REZNAROVA, Jan VOTYPKA, Donnamae KLOCEK et. al.

Základní údaje

Originální název

Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

Autoři

MACEDO, Diego H, Danyil GRYBCHUK (804 Ukrajina, domácí), Jana REZNAROVA, Jan VOTYPKA, Donnamae KLOCEK, Tatiana YURCHENKO, Jan SEVCIK, Alice MAGRI, Michaela Urda DOLINSKA, Kristina ZAHONOVA, Julius LUKES, Elena SERVIENE, Alexandra JASZAYOVA, Saulius SERVA, Marina N MALYSHEVA, Alexander O FROLOV, Vyacheslav YURCHENKO a Alexei Yu KOSTYGOV (garant)

Vydání

BMC Biology, London, BMC, 2023, 1741-7007

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.400 v roce 2022

Kód RIV

RIV/00216224:14740/23:00133505

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1186/s12915-023-01687-y

UT WoS

001065813700002

Klíčová slova anglicky

Tombus-like viruses; Ostravirus; Leishbuviridae; Qinviridae; Pyrrhocoris apterus

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 09:12, Mgr. Eva Dubská

Anotace

V originále

Background Trypanosomatids are parasitic flagellates well known because of some representatives infecting humans, domestic animals, and cultural plants. Many trypanosomatid species bear RNA viruses, which, in the case of human pathogens Leishmania spp., influence the course of the disease. One of the close relatives of leishmaniae, Leptomonas pyrrhocoris, has been previously shown to harbor viruses of the groups not documented in other trypanosomatids. At the same time, this species has a worldwide distribution and high prevalence in the natural populations of its cosmopolitan firebug host. It therefore represents an attractive model to study the diversity of RNA viruses.Results We surveyed 106 axenic cultures of L. pyrrhocoris and found that 64 (60%) of these displayed 2-12 double-stranded RNA fragments. The analysis of next-generation sequencing data revealed four viral groups with seven species, of which up to five were simultaneously detected in a single trypanosomatid isolate. Only two of these species, a tombus-like virus and an Ostravirus, were earlier documented in L. pyrrhocoris. In addition, there were four new species of Leishbuviridae, the family encompassing trypanosomatid-specific viruses, and a new species of Qinviridae, the family previously known only from metatranscriptomes of invertebrates. Currently, this is the only qinvirus with an unambiguously determined host. Our phylogenetic inferences suggest reassortment in the tombus-like virus owing to the interaction of different trypanosomatid strains. Two of the new Leishbuviridae members branch early on the phylogenetic tree of this family and display intermediate stages of genomic segment reduction between insect Phenuiviridae and crown Leishbuviridae.Conclusions The unprecedented wide range of viruses in one protist species and the simultaneous presence of up to five viral species in a single Leptomonas pyrrhocoris isolate indicate the uniqueness of this flagellate. This is likely determined by the peculiarity of its firebug host, a highly abundant cosmopolitan species with several habits ensuring wide distribution and profuseness of L. pyrrhocoris, as well as its exposure to a wider spectrum of viruses compared to other trypanosomatids combined with a limited ability to transmit these viruses to its relatives. Thus, L. pyrrhocoris represents a suitable model to study the adoption of new viruses and their relationships with a protist host.

Návaznosti

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

MACEDO, Diego H, Danyil GRYBCHUK, Jana REZNAROVA, Jan VOTYPKA, Donnamae KLOCEK, Tatiana YURCHENKO, Jan SEVCIK, Alice MAGRI, Michaela Urda DOLINSKA, Kristina ZAHONOVA, Julius LUKES, Elena SERVIENE, Alexandra JASZAYOVA, Saulius SERVA, Marina N MALYSHEVA, Alexander O FROLOV, Vyacheslav YURCHENKO a Alexei Yu KOSTYGOV. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris. BMC Biology. London: BMC, 2023, roč. 21, č. 1, s. 1-18. ISSN 1741-7007. Dostupné z: https://dx.doi.org/10.1186/s12915-023-01687-y.

@article{2372137,
   author = {Macedo, Diego H and Grybchuk, Danyil and Reznarova, Jana and Votypka, Jan and Klocek, Donnamae and Yurchenko, Tatiana and Sevcik, Jan and Magri, Alice and Dolinska, Michaela Urda and Zahonova, Kristina and Lukes, Julius and Serviene, Elena and Jaszayova, Alexandra and Serva, Saulius and Malysheva, Marina N and Frolov, Alexander O and Yurchenko, Vyacheslav and Kostygov, Alexei Yu},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12915-023-01687-y},
   keywords = {Tombus-like viruses; Ostravirus; Leishbuviridae; Qinviridae; Pyrrhocoris apterus},
   language = {eng},
   issn = {1741-7007},
   journal = {BMC Biology},
   title = {Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris},
   url = {https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-023-01687-y},
   volume = {21},
   year = {2023}
}

TY  - JOUR
ID  - 2372137
AU  - Macedo, Diego H - Grybchuk, Danyil - Reznarova, Jana - Votypka, Jan - Klocek, Donnamae - Yurchenko, Tatiana - Sevcik, Jan - Magri, Alice - Dolinska, Michaela Urda - Zahonova, Kristina - Lukes, Julius - Serviene, Elena - Jaszayova, Alexandra - Serva, Saulius - Malysheva, Marina N - Frolov, Alexander O - Yurchenko, Vyacheslav - Kostygov, Alexei Yu
PY  - 2023
TI  - Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris
JF  - BMC Biology
VL  - 21
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - BMC
SN  - 17417007
KW  - Tombus-like viruses
KW  - Ostravirus
KW  - Leishbuviridae
KW  - Qinviridae
KW  - Pyrrhocoris apterus
UR  - https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-023-01687-y
N2  - Background Trypanosomatids are parasitic flagellates well known because of some representatives infecting humans, domestic animals, and cultural plants. Many trypanosomatid species bear RNA viruses, which, in the case of human pathogens Leishmania spp., influence the course of the disease. One of the close relatives of leishmaniae, Leptomonas pyrrhocoris, has been previously shown to harbor viruses of the groups not documented in other trypanosomatids. At the same time, this species has a worldwide distribution and high prevalence in the natural populations of its cosmopolitan firebug host. It therefore represents an attractive model to study the diversity of RNA viruses.Results We surveyed 106 axenic cultures of L. pyrrhocoris and found that 64 (60%) of these displayed 2-12 double-stranded RNA fragments. The analysis of next-generation sequencing data revealed four viral groups with seven species, of which up to five were simultaneously detected in a single trypanosomatid isolate. Only two of these species, a tombus-like virus and an Ostravirus, were earlier documented in L. pyrrhocoris. In addition, there were four new species of Leishbuviridae, the family encompassing trypanosomatid-specific viruses, and a new species of Qinviridae, the family previously known only from metatranscriptomes of invertebrates. Currently, this is the only qinvirus with an unambiguously determined host. Our phylogenetic inferences suggest reassortment in the tombus-like virus owing to the interaction of different trypanosomatid strains. Two of the new Leishbuviridae members branch early on the phylogenetic tree of this family and display intermediate stages of genomic segment reduction between insect Phenuiviridae and crown Leishbuviridae.Conclusions The unprecedented wide range of viruses in one protist species and the simultaneous presence of up to five viral species in a single Leptomonas pyrrhocoris isolate indicate the uniqueness of this flagellate. This is likely determined by the peculiarity of its firebug host, a highly abundant cosmopolitan species with several habits ensuring wide distribution and profuseness of L. pyrrhocoris, as well as its exposure to a wider spectrum of viruses compared to other trypanosomatids combined with a limited ability to transmit these viruses to its relatives. Thus, L. pyrrhocoris represents a suitable model to study the adoption of new viruses and their relationships with a protist host.
ER  -

MACEDO, Diego H, Danyil GRYBCHUK, Jana REZNAROVA, Jan VOTYPKA, Donnamae KLOCEK, Tatiana YURCHENKO, Jan SEVCIK, Alice MAGRI, Michaela Urda DOLINSKA, Kristina ZAHONOVA, Julius LUKES, Elena SERVIENE, Alexandra JASZAYOVA, Saulius SERVA, Marina N MALYSHEVA, Alexander O FROLOV, Vyacheslav YURCHENKO a Alexei Yu KOSTYGOV. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris. \textit{BMC Biology}. London: BMC, 2023, roč.~21, č.~1, s.~1-18. ISSN~1741-7007. Dostupné z: https://dx.doi.org/10.1186/s12915-023-01687-y.

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