Production of recombinant lectin PluLec from Photorhabdus
luminescens for neutron crystallography and its
structure-functional characterization.

k 2023

Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization.

KORSÁK, Marek, Josef HOUSER, Juliette DEVOS, Lukáš GAJDOŠ, Pavel DOBEŠ et. al.

Základní údaje

Originální název

Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization.

Název česky

Produkce rekombinantního lektinu PluLec z Photorhabdus luminescens pro neutronovou krystalografii a jeho strukturně-funkční charakterizace

Autoři

KORSÁK, Marek, Josef HOUSER, Juliette DEVOS, Lukáš GAJDOŠ, Pavel DOBEŠ a Michaela WIMMEROVÁ

Vydání

XXVIIth Biochemistry Congress of Slovak and Czech Societies for Biochemistry and Molecular Biology with cooperation of Hungarian and Ukrainian Biochemical Societies, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-8240-047-5

Klíčová slova česky

lektin; strukturně-funkční analýza; neutronová krystalografie

Klíčová slova anglicky

lectin; structure-functional analysis, neutron crystallography

Příznaky

Mezinárodní význam

Změněno: 12. 2. 2024 13:48, Mgr. Marek Korsák

Anotace

V originále

Lectins are ubiquitous proteins of non-immune origin that reversibly and specifically interact with carbohydrates. They play a role in various physiological and pathological processes like intercellular communication, adhesion, migration and host-pathogen interactions. Unlike antibodies, they are not products of immune response and lack enzymatic activity. Lectins are widely used for carbohydrate structure characterization, glycoprotein purification, and specific labeling of cell surface structures. Photorhabdus luminescens is a bioluminescent Gram-negative bacterium and an insect pathogen that symbiotically lives in Heterorhabditidae nematodes. PluLec, a putative lectin from Photorhabdus luminescens shares homology with PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on structural-functional characterization of recombinant protein PluLec using various methods like isothermal titration calorimetry, hemagglutination, glycan array, analytical ultracentrifugation, toxicity tests performed on insect models and protein X-ray and Neutron crystallography. Unraveling lectin’s atomic structure and interactions with carbohydrates is essential for understanding its function and potential applications. Neutron protein crystallography, with its unique ability to visualize hydrogen atom positions, offers a promising avenue for gaining deeper insights into lectin-carbohydrate recognition. However, the successful application of neutron crystallography requires producing sufficient quantities of isotopically labeled recombinant lectin and obtaining high-quality crystals suitable for neutron diffraction. Several considerations, such as selecting an appropriate expression system, optimizing expression and crystallization conditions, need to be taken into account. The study revealed that lectin crystallizes as a homotetramer with four binding sites for D-galactose (one per monomer) and shows specificity towards beta anomers of D-galactose. Preliminary results from toxicity tests conducted on insect models indicate a clear negative effect on insect survival. The obtained results of the structure and function of PluLec may reveal its importance in the pathogenic or symbiotic stage of life. Neutron diffraction experiments can deepen our understanding of lectin functionality, aid in the design of therapeutic strategies targeting lectins, and potentially inspire the development of novel carbohydrate-based therapeutics.

Návaznosti

GA21-29622S, projekt VaV

Název: Biologická úloha lektinů v symbióze a patogenezi

Investor: Grantová agentura ČR, Biologická úloha lektinů v symbióze a patogenezi

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

Citovat

KORSÁK, Marek, Josef HOUSER, Juliette DEVOS, Lukáš GAJDOŠ, Pavel DOBEŠ a Michaela WIMMEROVÁ. Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization. In XXVIIth Biochemistry Congress of Slovak and Czech Societies for Biochemistry and Molecular Biology with cooperation of Hungarian and Ukrainian Biochemical Societies. 2023. ISBN 978-80-8240-047-5.

@proceedings{2373121,
   author = {Korsák, Marek and Houser, Josef and Devos, Juliette and Gajdoš, Lukáš and Dobeš, Pavel and Wimmerová, Michaela},
   booktitle = {XXVIIth Biochemistry Congress of Slovak and Czech Societies for Biochemistry and Molecular Biology with cooperation of Hungarian and Ukrainian Biochemical Societies},
   keywords = {lectin; structure-functional analysis, neutron crystallography},
   language = {eng},
   isbn = {978-80-8240-047-5},
   title = {Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization.},
   year = {2023}
}

TY  - CONF
ID  - 2373121
AU  - Korsák, Marek - Houser, Josef - Devos, Juliette - Gajdoš, Lukáš - Dobeš, Pavel - Wimmerová, Michaela
PY  - 2023
TI  - Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization.
SN  - 9788082400475
KW  - lectin
KW  - structure-functional analysis, neutron crystallography
N2  - Lectins are ubiquitous proteins of non-immune origin that reversibly and specifically interact with carbohydrates. They play a role in various physiological and pathological processes like intercellular communication, adhesion, migration and host-pathogen interactions. Unlike antibodies, they are not products of immune response and lack enzymatic activity. Lectins are widely used for carbohydrate structure characterization, glycoprotein purification, and specific labeling of cell surface structures. Photorhabdus luminescens is a bioluminescent Gram-negative bacterium and an insect pathogen that symbiotically lives in Heterorhabditidae nematodes. PluLec, a putative lectin from Photorhabdus luminescens shares homology with PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on structural-functional characterization of recombinant protein PluLec using various methods like isothermal titration calorimetry, hemagglutination, glycan array, analytical ultracentrifugation, toxicity tests performed on insect models and protein X-ray and Neutron crystallography. Unraveling lectin’s atomic structure and interactions with carbohydrates is essential for understanding its function and potential applications. Neutron protein crystallography, with its unique ability to visualize hydrogen atom positions, offers a promising avenue for gaining deeper insights into lectin-carbohydrate recognition. However, the successful application of neutron crystallography requires producing sufficient quantities of isotopically labeled recombinant lectin and obtaining high-quality crystals suitable for neutron diffraction. Several considerations, such as selecting an appropriate expression system, optimizing expression and crystallization conditions, need to be taken into account. The study revealed that lectin crystallizes as a homotetramer with four binding sites for D-galactose (one per monomer) and shows specificity towards beta anomers of D-galactose. Preliminary results from toxicity tests conducted on insect models indicate a clear negative effect on insect survival. The obtained results of the structure and function of PluLec may reveal its importance in the pathogenic or symbiotic stage of life. Neutron diffraction experiments can deepen our understanding of lectin functionality, aid in the design of therapeutic strategies targeting lectins, and potentially inspire the development of novel carbohydrate-based therapeutics.
ER  -

KORSÁK, Marek, Josef HOUSER, Juliette DEVOS, Lukáš GAJDOŠ, Pavel DOBEŠ a Michaela WIMMEROVÁ. Production of recombinant lectin PluLec from Photorhabdus luminescens for neutron crystallography and its structure-functional characterization. In \textit{XXVIIth Biochemistry Congress of Slovak and Czech Societies for Biochemistry and Molecular Biology with cooperation of Hungarian and Ukrainian Biochemical Societies}. 2023. ISBN~978-80-8240-047-5.

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