Bacteriome of bronchoalveolar lavage in patients with
inhalation injury during their hospitalization - a pilot study

a 2023

Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

VYKLICKÁ, Kateřina, Jan BÖHM, Petra BRENEROVÁ, Břetislav LIPOVÝ, Petra BOŘILOVÁ LINHARTOVÁ et. al.

Základní údaje

Originální název

Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study

Autoři

VYKLICKÁ, Kateřina (203 Česká republika), Jan BÖHM (203 Česká republika), Petra BRENEROVÁ (203 Česká republika), Břetislav LIPOVÝ (203 Česká republika) a Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, garant, domácí)

Vydání

World of Microbiome, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Bulharsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/23:00133529

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

inhalation injury; airways; respiratory tract; bacteriome; 16S rDNA sequencing

Změněno: 14. 2. 2024 12:15, Mgr. Terezie Slámová

Anotace

V originále

Background and Aims Inhalation injury (INHI) is defined as acute airway injury caused by inhalation of hot steam and/or products of combustion. During the hospitalization of these patients, commensal bacterial populations colonizing the lungs, such as Prevotella spp. and Veillonella spp., are displaced by bacteria with pathogenic potential, such as Pseudomonas aeruginosa and Klebsiella pneumonia. In our pilot study, we aimed to determine the dynamic changes of the bacteriome in 10 patients with INHI during their hospitalization. Methods We characterized the bacteriome from oral and oropharyngeal swabs, bronchoalveolar lavage (BAL), catheter urine, and blood using 16S rDNA sequencing at 6 time-points of hospitalization (Days 1 - 28). Results Oral and oropharyngeal swabs revealed high abundance of bacterial DNA. The bacterial composition was quite stable among the studied time-points, and both their alpha- and beta-diversities were similar in similar matrices. The BAL bacteriome composition was related to the oral bacteriome of respective patients; high abundances of Klebsiella sp., Enterobacter sp., Haemophillus sp., Escherichia sp., Staphylococcus sp., Pseudomonas sp., and Neisseria sp. were found in BAL. The results showed that the urine and blood samples were almost sterile at the beginning of the hospitalization; however, in some cases, low amounts of Klebsiella sp. and high abundance of Proteus sp. or Escherichia sp. were found in the blood and urine samples, respectively. Conclusion To conclude, the oral bacteriome seems to be a source of bacterial lung infection in patients with INHI during their hospitalization. Therefore, the oral microbiota could serve as potential a screening marker for the lung microbiome.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

VYKLICKÁ, Kateřina, Jan BÖHM, Petra BRENEROVÁ, Břetislav LIPOVÝ a Petra BOŘILOVÁ LINHARTOVÁ. Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study. In World of Microbiome. 2023.

@proceedings{2373884,
   author = {Vyklická, Kateřina and Böhm, Jan and Brenerová, Petra and Lipový, Břetislav and Bořilová Linhartová, Petra},
   booktitle = {World of Microbiome},
   keywords = {inhalation injury; airways; respiratory tract; bacteriome; 16S rDNA sequencing},
   language = {eng},
   title = {Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study},
   year = {2023}
}

TY  - CONF
ID  - 2373884
AU  - Vyklická, Kateřina - Böhm, Jan - Brenerová, Petra - Lipový, Břetislav - Bořilová Linhartová, Petra
PY  - 2023
TI  - Bacteriome of bronchoalveolar lavage in patients with inhalation injury during their hospitalization - a pilot study
KW  - inhalation injury
KW  - airways
KW  - respiratory tract
KW  - bacteriome
KW  - 16S rDNA sequencing
N2  - Background and Aims Inhalation injury (INHI) is defined as acute airway injury caused by inhalation of hot steam and/or products of combustion. During the hospitalization of these patients, commensal bacterial populations colonizing the lungs, such as Prevotella spp. and Veillonella spp., are displaced by bacteria with pathogenic potential, such as Pseudomonas aeruginosa and Klebsiella pneumonia. In our pilot study, we aimed to determine the dynamic changes of the bacteriome in 10 patients with INHI during their hospitalization. Methods We characterized the bacteriome from oral and oropharyngeal swabs, bronchoalveolar lavage (BAL), catheter urine, and blood using 16S rDNA sequencing at 6 time-points of hospitalization (Days 1 - 28). Results Oral and oropharyngeal swabs revealed high abundance of bacterial DNA. The bacterial composition was quite stable among the studied time-points, and both their alpha- and beta-diversities were similar in similar matrices. The BAL bacteriome composition was related to the oral bacteriome of respective patients; high abundances of Klebsiella sp., Enterobacter sp., Haemophillus sp., Escherichia sp., Staphylococcus sp., Pseudomonas sp., and Neisseria sp. were found in BAL. The results showed that the urine and blood samples were almost sterile at the beginning of the hospitalization; however, in some cases, low amounts of Klebsiella sp. and high abundance of Proteus sp. or Escherichia sp. were found in the blood and urine samples, respectively. Conclusion To conclude, the oral bacteriome seems to be a source of bacterial lung infection in patients with INHI during their hospitalization. Therefore, the oral microbiota could serve as potential a screening marker for the lung microbiome.
ER  -

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