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@proceedings{2374417,
author = {Bořilová Linhartová, Petra and Száraz, Dávid and Cerulová, Sabina and Bodokyová, Lenka and Macháček, Ctirad and Daněk, Zdeněk and Brenerová, Petra and Budinská, Eva},
booktitle = {3rd International Conference on Oral Mucosal Immunity and Microbiome},
keywords = {bacteriome; cystic fluids; odontogenic cysts},
language = {eng},
title = {Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study},
year = {2023}
}
TY - CONF
ID - 2374417
AU - Bořilová Linhartová, Petra - Száraz, Dávid - Cerulová, Sabina - Bodokyová, Lenka - Macháček, Ctirad - Daněk, Zdeněk - Brenerová, Petra - Budinská, Eva
PY - 2023
TI - Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study
KW - bacteriome
KW - cystic fluids
KW - odontogenic cysts
N2 - The diagnosis of developmental odontogenic cyst (OC) is not always clear; it may show inflammatory changes, and in that case the diagnostic features can be lost. As developmental OCs can become secondarily infected, the aim of this pilot study was to investigate bacteriomes of fluids from developmental OCs (e.g. dentigerous cyst, DC; odontogenic keratocyst, OKC) with/without inflammatory changes and compare them with findings in fluids from inflammatory OCs (radicular cyst, RC). Cystic fluids from 38 DCs, 12 OKCs, and 34 RCs were obtained by aspiration using a sterile syringe. Microbial DNA was isolated from cystic fluids and negative controls (N=32) using the QIAamp DNA Mini Kit; isolates were spiked with a mock community. 16S rRNA gene libraries were sequenced on Illumina MiSeq instrument with ≥5000 reads per sample. A sample with a relative abundance of a bacterial genus >20% was considered “positive”. Alpha diversity was significantly decreased in fluids from OCs with inflammation (N=42) in comparison with those without inflammation (N=42; Shannon index, p<0.05). Bacteriomes of fluids from DCs without inflammatory changes were similar to negative controls (p>0.05). Interestingly, 75% of fluids from OKCs were “positive” for DNA of one or more bacterial genera (e.g. Porphyromonas, Prevotella, Parvimonas, Haemophilus). Significant difference in the representation of "positive" samples was observed between those from DC and RC (26% vs. 56% of samples, p<0.05). RC fluids were “positive” for Fusobacterium, Porphyromonas, Prevotella, Fretibacterium, Aggregatibacter, and others. In conclusion, non-sterile cystic fluids from inflammatory DCs, OKCs, and RCs contain DNA from anaerobic periodontal pathogens. These bacteria might play a role in OKCs’ development. In line with our assumption, the content of DCs without inflammatory changes appears to be sterile in most cases.
ER -
BOŘILOVÁ LINHARTOVÁ, Petra, Dávid SZÁRAZ, Sabina CERULOVÁ, Lenka BODOKYOVÁ, Ctirad MACHÁČEK, Zdeněk DANĚK, Petra BRENEROVÁ a Eva BUDINSKÁ. Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study. In \textit{3rd International Conference on Oral Mucosal Immunity and Microbiome}. 2023.
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