Bacteriomes of dental plaques from teeth affected by apical periodontitis in patients with different periapical lesions: a pilot case-case study

BOŘILOVÁ LINHARTOVÁ, Petra, Dávid SZÁRAZ, Sabina CERULOVÁ, Lenka BODOKYOVÁ, Ctirad MACHÁČEK, Zdeněk DANĚK and Eva BUDINSKÁ. Bacteriomes of dental plaques from teeth affected by apical periodontitis in patients with different periapical lesions: a pilot case-case study. In 3rd International Conference on Microbiology and Immunology. 2023.

Basic information

• Original name: Bacteriomes of dental plaques from teeth affected by apical periodontitis in patients with different periapical lesions: a pilot case-case study
• Authors: BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Dávid SZÁRAZ (203 Czech Republic), Sabina CERULOVÁ (203 Czech Republic), Lenka BODOKYOVÁ (703 Slovakia), Ctirad MACHÁČEK (203 Czech Republic), Zdeněk DANĚK (203 Czech Republic) and Eva BUDINSKÁ (703 Slovakia).
• Edition: 3rd International Conference on Microbiology and Immunology, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United Arab Emirates
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/23:00133543
• Organization unit: Faculty of Science
• Keywords in English: bacteriome; dental plaque; apical periodontitis

Abstract

Background: Apical periodontitis (AP), an acute or chronic inflammation of the root canal and periradicular tissues, is caused by oral bacteria. AP leads to the development of a periapical lesion such as periapical granuloma (G) and radicular cyst (RC). Unlike RCs, Gs tend to heal even without surgical root canal treatment. The role of oral bacteria in the process of G and RC development is unclear. Objective: To compare the bacteriomes of dental plaque from teeth affected by AP between patients with G and those with RC. Methods: Dental plaque swabs were collected from 51 patients with AP+G and 54 patients with AP+RC. All patients were clinically, radiographically, and histopathologically examined. Microbial DNA was isolated from swabs and negative controls (NC=42, DNA-free water) using the QIAamp DNA Mini Kit; isolates were spiked with a mock community. 16S rRNA sequencing was performed on the Illumina MiSeq instrument with ≥5000 reads per sample. Results: In total, 104 dental plaques were found highly abundant for the content of bacterial DNA (one sample from a patient with AP+RC had a similar profile with NCs). The number of amplicon sequence variants and alpha diversities (Shannon index) were similar between cases (p>0.05), but significantly differed from NC (q<0.001, Kruskal-Wallis and Dunn´s test). Streptococcus, Actinomyces, Rothia, and Veillonella were the most common genera in dental plaques from teeth affected by AP. The relative abundance of the Gemella genus was significantly higher in samples from patients with AP+RC than in those with AP+G as well as NC (q=0.03, q<0.001, respectively, Kruskal-Wallis and Dunn´s test). Conclusion: Bacteriomes of dental plaques from patients with AP+G and AP+RC were similar in almost all observed characteristics, with Gemella being the only genus significantly more represented in patients with RC compared to those with G.

Links

• EF17_043/0009632, research and development project: Name: CETOCOEN Excellence
• LM2023069, research and development project: Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure

• NU20-08-00205, research and development project: Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministry of Health of the CR, Subprogram 1 - standard

• 857560, interní kód MU(CEP code: EF17_043/0009632): Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation