2023
Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment
KLVAŇOVÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ et. al.Základní údaje
Originální název
Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment
Autoři
KLVAŇOVÁ, Eva (203 Česká republika, domácí), Petra VÍDEŇSKÁ (203 Česká republika), Lucie BUREŠOVÁ (203 Česká republika), Milan URÍK (703 Slovensko), Soňa SMETANOVÁ (203 Česká republika), Stanislav SMATANA (703 Slovensko), Roman PROKEŠ (203 Česká republika), Barbara LÁNÍČKOVÁ (203 Česká republika), Eva BUDINSKÁ (703 Slovensko), Jana KLÁNOVÁ (203 Česká republika) a Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, garant)
Vydání
Setkání biochemiků a molekulárních biologů, 2023
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/23:00133550
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
bacteriome; dust; nasopharynx; household; hospital; sequencing; 16S rRNA; exposure; indoor environment
Změněno: 16. 2. 2024 13:05, Mgr. Terezie Slámová
Anotace
V originále
Objective: Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments. Methods: Nasopharyngeal swabs were taken from 22 participants in the morning and after 8 hours of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants’ households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital – NEO, 6 from a pediatric hospital – ENT, and 8 from a research center – RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices. Results: Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 hours of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76 % of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These “enriching” bacterial genera overlapped between the hospital workplaces – NEO and ENT but differed from those in the research center – RCX. Conclusions: The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day, short-term exposure to the indoor environment can result in its enrichment with bacterial DNA from indoor dust, especially from hospitals.
Návaznosti
EF17_043/0009632, projekt VaV |
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LM2018132, projekt VaV |
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LM2023069, projekt VaV |
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