The lack of association of selected interleukin 10 polymorphisms with odontogenic cysts in the Czech population

BODOKYOVÁ, Lenka, Dávid SZÁRAZ, Zdeněk DANĚK, Ctirad MACHÁČEK a Petra BOŘILOVÁ LINHARTOVÁ. The lack of association of selected interleukin 10 polymorphisms with odontogenic cysts in the Czech population. In Setkání biochemiků a molekulárních biologů. 2023.

Základní údaje

• Originální název: The lack of association of selected interleukin 10 polymorphisms with odontogenic cysts in the Czech population
• Autoři: BODOKYOVÁ, Lenka (703 Slovensko), Dávid SZÁRAZ (203 Česká republika), Zdeněk DANĚK (203 Česká republika), Ctirad MACHÁČEK (203 Česká republika) a Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, garant, domácí).
• Vydání: Setkání biochemiků a molekulárních biologů, 2023.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10608 Biochemistry and molecular biology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/23:00133551
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: interleukin; polymorphisms; odontogenic cysts

Anotace

Genetic predispositions play a role in the etiopathogenesis of both inflammatory and developmental odontogenic cysts (OCs). A radicular cyst (RC) is an inflammatory OC, often linked with apical periodontitis (AP) caused by a microbial infection within the root canal system. Dentigerous cysts (DC) and keratocysts (OKC) are the most common developmental OCs; inflammation is also present in some OCs of developmental origin. Another type of neoplasms of odontogenic origin, ameloblastoma (AME), is benign but can be locally aggressive. Previously, an association of aggressive periodontitis with single nucleotide polymorphisms (SNPs) in the interleukin 10, an anti-inflammatory cytokine, was found in the Czech population. Our research aimed to find an association between these IL-10 SNPs and OCs in the Czech population. This genetic association study included 248 patients: 88 with AP without RC, 85 with AP and RC, 53 with DC, 16 with OKC, and 6 with AME. DNA was extracted from peripheral blood leukocytes using the phenol-chloroform method. All three studied SNPs, namely IL-10 −1087 A/G (rs1800896), IL-10 −824 C/T (rs1800871), and IL-10 −597 C/A (rs1800872) are located in the promoter region and may have the ability to modify transcription factor binding sites, thus affecting gene expression. PCR followed by a restriction fragment length polymorphism method (PCR-RFLP) was used for genotyping the SNPs. In our research, the genotype distributions of all studied SNPs in patients were in Hardy-Weinberg equilibrium (p > 0.05); minor allele frequencies were in line with frequencies in the European population. We found no evidence of significant differences between either genotype as well as allele frequencies of the analyzed IL-10 polymorphisms among patient groups with different diagnoses (p > 0.05). In conclusion, we have not found any evidence of association of these IL10 SNPs with the predisposition to both inflammatory and developmental OCs in the Czech population.

Návaznosti

• EF17_043/0009632, projekt VaV: Název: CETOCOEN Excellence
• LM2023069, projekt VaV: Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

• NU20-08-00205, projekt VaV: Název: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministerstvo zdravotnictví ČR, Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst, Podprogram 1 - standardní

• 857560, interní kód MU(Kód CEP: EF17_043/0009632): Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation