ISG20L2: an RNA nuclease regulating T cell activation

J 2023

ISG20L2: an RNA nuclease regulating T cell activation

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ et. al.

Základní údaje

Originální název

ISG20L2: an RNA nuclease regulating T cell activation

Autoři

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ (203 Česká republika, domácí), Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ (703 Slovensko, domácí), Julie POKORNÁ (203 Česká republika, domácí), Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ (203 Česká republika, domácí) a Francisco SANCHEZ-MADRID (garant)

Vydání

Cellular and molecular life sciences, BASEL, BIRKHAUSER VERLAG AG, 2023, 1420-682X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.000 v roce 2022

Kód RIV

RIV/00216224:14740/23:00133564

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00018-023-04925-2

UT WoS

001058609400002

Klíčová slova anglicky

Exonuclease; T cell; Immunoregulatory

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 8. 2024 13:50, Mgr. Eva Dubská

Anotace

V originále

ISG20L2, a 3' to 5' exoribonuclease previously associated with ribosome biogenesis, is identified here in activated T cells as an enzyme with a preferential affinity for uridylated miRNA substrates. This enzyme is upregulated in T lymphocytes upon TCR and IFN type I stimulation and appears to be involved in regulating T cell function. ISG20L2 silencing leads to an increased basal expression of CD69 and induces greater IL2 secretion. However, ISG20L2 absence impairs CD25 upregula-tion, CD3 synaptic accumulation and MTOC translocation towards the antigen-presenting cell during immune synapsis. Remarkably, ISG20L2 controls the expression of immunoregulatory molecules, such as AHR, NKG2D, CTLA-4, CD137, TIM-3, PD-L1 or PD-1, which show increased levels in ISG20L2 knockout T cells. The dysregulation observed in these key molecules for T cell responses support a role for this exonuclease as a novel RNA-based regulator of T cell function.

Návaznosti

GA20-19617S, projekt VaV

Název: Molekulární mechanismy nekanonických 3' terminálních modifikací RNA a jejich úloha v metabolismu kódujících a nekódujících RNA

Investor: Grantová agentura ČR, The molecular mechanisms of noncanonical 3' terminal RNA tailing and its role in the metabolism of coding and noncoding RNAs

GA23-07372S, projekt VaV

Název: Identifikace a úloha proteinů v kontrole a degradaci RNA a regulaci genové exprese

Investor: Grantová agentura ČR, Identifikace a úloha proteinů v kontrole a degradaci RNA a regulaci genové exprese

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ, Julie POKORNÁ, Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ a Francisco SANCHEZ-MADRID. ISG20L2: an RNA nuclease regulating T cell activation. Cellular and molecular life sciences. BASEL: BIRKHAUSER VERLAG AG, 2023, roč. 80, č. 9, s. 1-19. ISSN 1420-682X. Dostupné z: https://dx.doi.org/10.1007/s00018-023-04925-2.

@article{2375338,
   author = {RodriguezandGalan, Ana and Dosil, Sara G and Vlčková, Anna and FernandezandMessina, Lola and Feketová, Zuzana and Pokorná, Julie and FernandezandDelgado, Irene and Camafeita, Emilio and Gomez, Manuel Jose and RamirezandHuesca, Marta and GutierrezandVazquez, Cristina and SanchezandCabo, Fatima and Vazquez, Jesus and Vaňáčová, Štěpánka and SanchezandMadrid, Francisco},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s00018-023-04925-2},
   keywords = {Exonuclease; T cell; Immunoregulatory},
   language = {eng},
   issn = {1420-682X},
   journal = {Cellular and molecular life sciences},
   title = {ISG20L2: an RNA nuclease regulating T cell activation},
   url = {https://link.springer.com/article/10.1007/s00018-023-04925-2},
   volume = {80},
   year = {2023}
}

TY  - JOUR
ID  - 2375338
AU  - Rodriguez-Galan, Ana - Dosil, Sara G - Vlčková, Anna - Fernandez-Messina, Lola - Feketová, Zuzana - Pokorná, Julie - Fernandez-Delgado, Irene - Camafeita, Emilio - Gomez, Manuel Jose - Ramirez-Huesca, Marta - Gutierrez-Vazquez, Cristina - Sanchez-Cabo, Fatima - Vazquez, Jesus - Vaňáčová, Štěpánka - Sanchez-Madrid, Francisco
PY  - 2023
TI  - ISG20L2: an RNA nuclease regulating T cell activation
JF  - Cellular and molecular life sciences
VL  - 80
IS  - 9
SP  - 1-19
EP  - 1-19
PB  - BIRKHAUSER VERLAG AG
SN  - 1420682X
KW  - Exonuclease
KW  - T cell
KW  - Immunoregulatory
UR  - https://link.springer.com/article/10.1007/s00018-023-04925-2
N2  - ISG20L2, a 3' to 5' exoribonuclease previously associated with ribosome biogenesis, is identified here in activated T cells as an enzyme with a preferential affinity for uridylated miRNA substrates. This enzyme is upregulated in T lymphocytes upon TCR and IFN type I stimulation and appears to be involved in regulating T cell function. ISG20L2 silencing leads to an increased basal expression of CD69 and induces greater IL2 secretion. However, ISG20L2 absence impairs CD25 upregula-tion, CD3 synaptic accumulation and MTOC translocation towards the antigen-presenting cell during immune synapsis. Remarkably, ISG20L2 controls the expression of immunoregulatory molecules, such as AHR, NKG2D, CTLA-4, CD137, TIM-3, PD-L1 or PD-1, which show increased levels in ISG20L2 knockout T cells. The dysregulation observed in these key molecules for T cell responses support a role for this exonuclease as a novel RNA-based regulator of T cell function.
ER  -

RODRIGUEZ-GALAN, Ana, Sara G DOSIL, Anna VLČKOVÁ, Lola FERNANDEZ-MESSINA, Zuzana FEKETOVÁ, Julie POKORNÁ, Irene FERNANDEZ-DELGADO, Emilio CAMAFEITA, Manuel Jose GOMEZ, Marta RAMIREZ-HUESCA, Cristina GUTIERREZ-VAZQUEZ, Fatima SANCHEZ-CABO, Jesus VAZQUEZ, Štěpánka VAŇÁČOVÁ a Francisco SANCHEZ-MADRID. ISG20L2: an RNA nuclease regulating T cell activation. \textit{Cellular and molecular life sciences}. BASEL: BIRKHAUSER VERLAG AG, 2023, roč.~80, č.~9, s.~1-19. ISSN~1420-682X. Dostupné z: https://dx.doi.org/10.1007/s00018-023-04925-2.

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