Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids

SADZAK, Anja, Zlatko BRKLJACA, Mihael ERAKOVIC, Manfred KRIECHBAUM, Nadica MALTAR-STRMECKI, Jan PŘIBYL a Suzana SEGOTA. Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids. JOURNAL OF LIPID RESEARCH. UNITED STATES: ELSEVIER, 2023, roč. 64, č. 10, s. 1-14. ISSN 0022-2275. Dostupné z: https://dx.doi.org/10.1016/j.jlr.2023.100430.

Základní údaje

• Originální název: Puncturing lipid membranes: onset of pore formation and the role of hydrogen bonding in the presence of flavonoids
• Autoři: SADZAK, Anja, Zlatko BRKLJACA, Mihael ERAKOVIC, Manfred KRIECHBAUM, Nadica MALTAR-STRMECKI, Jan PŘIBYL (203 Česká republika, domácí) a Suzana SEGOTA (garant).
• Vydání: JOURNAL OF LIPID RESEARCH, UNITED STATES, ELSEVIER, 2023, 0022-2275.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10608 Biochemistry and molecular biology
• Stát vydavatele: Nizozemské království
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 6.500 v roce 2022
• Kód RIV: RIV/00216224:14740/23:00133566
• Organizační jednotka: Středoevropský technologický institut
• Doi: http://dx.doi.org/10.1016/j.jlr.2023.100430
• UT WoS: 001083841600001
• Klíčová slova anglicky: Supplementary key words antioxidant; dicarboxylic acid; flavone; flavonol; lipid/peroxidation; oxidized lipid; phospholipid/phosphatidylcholine; physical biochemistry
• Štítky: CF NANO, rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Products of lipid peroxidation induce detrimental structural changes in cell membranes, such as the formation of water pores, which occur in the presence of lipids with partially oxidized chains. However, the influence of another class of products, dicarboxylic acids, is still unclear. These products have greater mobility in the lipid bilayer, which enables their aggregation and the formation of favorable sites for the appearance of pores. Therefore, dodecanedioic acid (DDA) was selected as a model product. Additionally, the influence of several structurally different flavonoids on DDA aggregation via formation of hydrogen bonds with carboxyl groups was investigated. The molecular dynamics of DDA in DOPC lipid bilayer revealed the formation of aggregates extending over the hydrophobic region of the bilayer and increasing its polarity. Consequently, water penetration and the appearance of water wires was observed, representing a new step in the mechanism of pore formation. Furthermore, DDA molecules were found to interact with lipid polar groups, causing them to be buried in the bilayer. The addition of flavonoids to the system disrupted aggregate formation, resulting in the displacement of DDA molecules from the center of the bilayer. The placement of DDA and flavonoids in the lipid bilayer was confirmed by small-angle X-ray scattering. Atomic force microscopy and electron paramagnetic resonance were used to characterize the structural properties. The presence of DDA increased bilayer roughness and decreased the ordering of lipid chains, surface, while flavonoids were found to reduce or reverse these changes.

Návaznosti

• LM2018127, projekt VaV: Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

• 964997, interní kód MU: Název: Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe (Akronym: A4L_ACTIONS)

Investor: Evropská unie, Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe, Health, demographic change and wellbeing (Societal Challenges)