Microstructural Analysis of Collagenous Structures in Relapsed
Clubfoot Tissue

J 2023

Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue

VONDRASEK, David, Daniel HADRABA, Jan PŘIBYL, Adam ECKHARDT, Martin OSTADAL et. al.

Basic information

Original name

Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue

Authors

VONDRASEK, David (guarantor), Daniel HADRABA, Jan PŘIBYL (203 Czech Republic, belonging to the institution), Adam ECKHARDT, Martin OSTADAL, Frantisek LOPOT, Karel JELEN, Martina DOUBKOVA, Jarmila KNITLOVA, Tomas NOVOTNY and Jiri JANACEK

Edition

Microscopy and Microanalysis, Saarbrücken, Cambridge University Press, 2023, 1431-9276

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20501 Materials engineering

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.800 in 2022

RIV identification code

RIV/00216224:14740/23:00133612

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/micmic/ozac012

UT WoS

001033590800026

Keywords in English

atomic force microscopy; label-free imaging; mechanical properties; multi-modal analysis; orthopedic surgery; relapsed clubfoot; talipes equinovarus congenitus

Abstract

V originále

Talipes equinovarus congenitus (clubfoot) is frequently defined as a stiff, contracted deformity, but few studies have described the tissue from the point of view of the extracellular matrix, and none have quantified its mechanical properties. Several researchers have observed that clubfoot exhibits signs of fibrosis in the medial side of the deformity that are absent in the lateral side. Our study aims to quantify the differences between the medial and lateral side tissue obtained from relapsed clubfoot during surgery in terms of the morphological and mechanical properties of the tissue. Combining methods of optical and atomic force microscopy, our study revealed that the medial side has a higher Young's modulus, contains more collagen and less adipose tissue and that the collagen fibers propagate at a higher frequency of the crimp pattern after surgical dissection of the tissue. Our study offers a multi-correlative approach that thoroughly investigates the relapsed clubfoot tissue.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

VONDRASEK, David, Daniel HADRABA, Jan PŘIBYL, Adam ECKHARDT, Martin OSTADAL, Frantisek LOPOT, Karel JELEN, Martina DOUBKOVA, Jarmila KNITLOVA, Tomas NOVOTNY and Jiri JANACEK. Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue. Microscopy and Microanalysis. Saarbrücken: Cambridge University Press, 2023, vol. 29, No 1, p. 265-272. ISSN 1431-9276. Available from: https://dx.doi.org/10.1093/micmic/ozac012.

@article{2376978,
   author = {Vondrasek, David and Hadraba, Daniel and Přibyl, Jan and Eckhardt, Adam and Ostadal, Martin and Lopot, Frantisek and Jelen, Karel and Doubkova, Martina and Knitlova, Jarmila and Novotny, Tomas and Janacek, Jiri},
   article_location = {Saarbrücken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/micmic/ozac012},
   keywords = {atomic force microscopy; label-free imaging; mechanical properties; multi-modal analysis; orthopedic surgery; relapsed clubfoot; talipes equinovarus congenitus},
   language = {eng},
   issn = {1431-9276},
   journal = {Microscopy and Microanalysis},
   title = {Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue},
   url = {https://academic.oup.com/mam/article/29/1/265/6948117?login=true},
   volume = {29},
   year = {2023}
}

TY  - JOUR
ID  - 2376978
AU  - Vondrasek, David - Hadraba, Daniel - Přibyl, Jan - Eckhardt, Adam - Ostadal, Martin - Lopot, Frantisek - Jelen, Karel - Doubkova, Martina - Knitlova, Jarmila - Novotny, Tomas - Janacek, Jiri
PY  - 2023
TI  - Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue
JF  - Microscopy and Microanalysis
VL  - 29
IS  - 1
SP  - 265-272
EP  - 265-272
PB  - Cambridge University Press
SN  - 14319276
KW  - atomic force microscopy
KW  - label-free imaging
KW  - mechanical properties
KW  - multi-modal analysis
KW  - orthopedic surgery
KW  - relapsed clubfoot
KW  - talipes equinovarus congenitus
UR  - https://academic.oup.com/mam/article/29/1/265/6948117?login=true
N2  - Talipes equinovarus congenitus (clubfoot) is frequently defined as a stiff, contracted deformity, but few studies have described the tissue from the point of view of the extracellular matrix, and none have quantified its mechanical properties. Several researchers have observed that clubfoot exhibits signs of fibrosis in the medial side of the deformity that are absent in the lateral side. Our study aims to quantify the differences between the medial and lateral side tissue obtained from relapsed clubfoot during surgery in terms of the morphological and mechanical properties of the tissue. Combining methods of optical and atomic force microscopy, our study revealed that the medial side has a higher Young's modulus, contains more collagen and less adipose tissue and that the collagen fibers propagate at a higher frequency of the crimp pattern after surgical dissection of the tissue. Our study offers a multi-correlative approach that thoroughly investigates the relapsed clubfoot tissue.
ER  -

VONDRASEK, David, Daniel HADRABA, Jan PŘIBYL, Adam ECKHARDT, Martin OSTADAL, Frantisek LOPOT, Karel JELEN, Martina DOUBKOVA, Jarmila KNITLOVA, Tomas NOVOTNY and Jiri JANACEK. Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2023, vol.~29, No~1, p.~265-272. ISSN~1431-9276. Available from: https://dx.doi.org/10.1093/micmic/ozac012.

Detailed Information on Publication Record