New sample preparation method to analyse 15 specific and
non-specific pesticide metabolites in human urine using
LC-MS/MS

J 2021

New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS

STUCHLÍK FIŠEROVÁ, Petra, Jiří KOHOUTEK, Céline DEGRENDELE, Mohamed Aqiel DALVIE, Jana KLÁNOVÁ et. al.

Základní údaje

Originální název

New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS

Autoři

STUCHLÍK FIŠEROVÁ, Petra (203 Česká republika, domácí), Jiří KOHOUTEK (203 Česká republika, garant, domácí), Céline DEGRENDELE (250 Francie, domácí), Mohamed Aqiel DALVIE a Jana KLÁNOVÁ (203 Česká republika, domácí)

Vydání

Journal of Chromatography B, Elsevier B.V. 2021, 1570-0232

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.318

Kód RIV

RIV/00216224:14310/21:00133630

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jchromb.2021.122542

UT WoS

001184688400001

Klíčová slova anglicky

Human biomonitoring; LC-MS; Pesticide metabolite; Sample preparation; Urine

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2024 09:28, Mgr. Marie Šípková, DiS.

Anotace

V originále

This study presents a novel sample preparation method for the determination of both specific and non-specific pesticide metabolites in human urine samples. The method combines a deconjugation step with QuEChERS-based method and solid-phase extraction. In total, 15 pesticide metabolites (diethyl phosphate; diethyl thiophosphate; dimethyl phosphate; diethyl thiophosphate; 2,4-dichlorophenoxyacetic acid; 3-phenoxybenzoic acid; 4-fluoro-3-phenoxybenzoic acid; coumaphos; diethyl dithiophosphate; malathion dicarboxylic acid; p-nitrophenol; cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid; 3,5,6-trichloro-2-pyridinol; N,N-diethyl-3-methylbenzamid and 2-isopropyl-4-methyl-6-hydroxypyrimidine) were separated using liquid chromatography coupled to a mass spectrometer and isotope dilution method for quantitation. The method was validated using recovery tests with recoveries generally ranging from 80 to 120%. Additionally, 20 urine samples collected from South African children were analysed using the presented method. The median levels of pesticide metabolites found in the urine samples ranged from not detected (N,N-diethyl-3-methylbenzamid) to 22.36 µg/g creatinine (dimethyl phosphate). The novel method developed in this study is sensitive, selective, robust and reproducible while also conserving the amount of sample, chemicals, material and time required. Due to the low limits of detection obtained for individual pesticide metabolites, the method is capable of quantifying trace levels of pesticide metabolites in urine, which thus makes it an ideal tool for biomonitoring studies.

Návaznosti

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

STUCHLÍK FIŠEROVÁ, Petra, Jiří KOHOUTEK, Céline DEGRENDELE, Mohamed Aqiel DALVIE a Jana KLÁNOVÁ. New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS. Journal of Chromatography B. Elsevier B.V., 2021, roč. 1166, March 2021, s. 1-8. ISSN 1570-0232. Dostupné z: https://dx.doi.org/10.1016/j.jchromb.2021.122542.

@article{2377409,
   author = {Stuchlík Fišerová, Petra and Kohoutek, Jiří and Degrendele, Céline and Dalvie, Mohamed Aqiel and Klánová, Jana},
   article_number = {March 2021},
   doi = {http://dx.doi.org/10.1016/j.jchromb.2021.122542},
   keywords = {Human biomonitoring; LC-MS; Pesticide metabolite; Sample preparation; Urine},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B},
   note = {Požádala jsem o přidání na WoS.},
   title = {New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS},
   url = {https://www.sciencedirect.com/science/article/pii/S1570023221000210},
   volume = {1166},
   year = {2021}
}

TY  - JOUR
ID  - 2377409
AU  - Stuchlík Fišerová, Petra - Kohoutek, Jiří - Degrendele, Céline - Dalvie, Mohamed Aqiel - Klánová, Jana
PY  - 2021
TI  - New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS
JF  - Journal of Chromatography B
VL  - 1166
IS  - March 2021
SP  - 1-8
EP  - 1-8
PB  - Elsevier B.V.
SN  - 15700232
N1  - Požádala jsem o přidání na WoS.
KW  - Human biomonitoring
KW  - LC-MS
KW  - Pesticide metabolite
KW  - Sample preparation
KW  - Urine
UR  - https://www.sciencedirect.com/science/article/pii/S1570023221000210
N2  - This study presents a novel sample preparation method for the determination of both specific and non-specific pesticide metabolites in human urine samples. The method combines a deconjugation step with QuEChERS-based method and solid-phase extraction. In total, 15 pesticide metabolites (diethyl phosphate; diethyl thiophosphate; dimethyl phosphate; diethyl thiophosphate; 2,4-dichlorophenoxyacetic acid; 3-phenoxybenzoic acid; 4-fluoro-3-phenoxybenzoic acid; coumaphos; diethyl dithiophosphate; malathion dicarboxylic acid; p-nitrophenol; cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid; 3,5,6-trichloro-2-pyridinol; N,N-diethyl-3-methylbenzamid and 2-isopropyl-4-methyl-6-hydroxypyrimidine) were separated using liquid chromatography coupled to a mass spectrometer and isotope dilution method for quantitation. The method was validated using recovery tests with recoveries generally ranging from 80 to 120%. Additionally, 20 urine samples collected from South African children were analysed using the presented method. The median levels of pesticide metabolites found in the urine samples ranged from not detected (N,N-diethyl-3-methylbenzamid) to 22.36 µg/g creatinine (dimethyl phosphate). The novel method developed in this study is sensitive, selective, robust and reproducible while also conserving the amount of sample, chemicals, material and time required. Due to the low limits of detection obtained for individual pesticide metabolites, the method is capable of quantifying trace levels of pesticide metabolites in urine, which thus makes it an ideal tool for biomonitoring studies.
ER  -

STUCHLÍK FIŠEROVÁ, Petra, Jiří KOHOUTEK, Céline DEGRENDELE, Mohamed Aqiel DALVIE a Jana KLÁNOVÁ. New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS. \textit{Journal of Chromatography B}. Elsevier B.V., 2021, roč.~1166, March 2021, s.~1-8. ISSN~1570-0232. Dostupné z: https://dx.doi.org/10.1016/j.jchromb.2021.122542.

Podrobný výpis o publikaci