Cyanobacterial anatoxin-a does not induce in vitro
developmental neurotoxicity, but changes gene expression
patterns in co-exposure with all-trans retinoic acid

J 2024

Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid

KUBÍČKOVÁ, Barbara, Šárka MARTINKOVÁ, Dáša BOHAČIAKOVÁ a Klára HILSCHEROVÁ

Základní údaje

Originální název

Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid

Autoři

KUBÍČKOVÁ, Barbara (203 Česká republika, domácí), Šárka MARTINKOVÁ (203 Česká republika, domácí), Dáša BOHAČIAKOVÁ (703 Slovensko, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Toxicology Letters, CLARE, Elsevier, 2024, 0378-4274

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.toxlet.2023.12.004

UT WoS

001138116200001

Klíčová slova anglicky

Developmental neurotoxicity; NSC; Retinoids; Differentiation; Anatoxin; Cyanotoxin

Štítky

14110517

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 15:27, Mgr. Marie Šípková, DiS.

Anotace

V originále

Cyanobacterial blooms are increasing in frequency and intensity globally, and impacting recreational waters as well as waters used for drinking water provisioning. They are sources of bioactive metabolites including retinoids and the neurotoxin anatoxin-a. Here, we investigated the effects of anatoxin-a on a differentiating in vitro human neural stem cell model previously characterised with retinoic acids. Effects on protein and gene expression upon exposure for 9 or 18 days to anatoxin-a alone or in co-exposure with all-trans retinoic acid were evaluated using a panel of neural and glial differentiation biomarkers. Anatoxin-a did not cause distinct developmental neuro-toxicity alone, or in co-exposure with retinoic acid. However, in line with its excitotoxicity, in co-exposure with 200 nM all-trans retinoic acid it reduced the differentiation of acetylcholinergic neuron subtypes in the culture at 1000 nM (highest tested concentration). While this could have substantial functional implications for the developing nervous system, there is no indication for developmental neurotoxicity beyond its (excito-)toxicity to acetylcholinergic neurons, which only occurred in co-exposure to all-trans retinoic acid.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2023069, projekt VaV

Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LX22NPO5107, projekt VaV

Název: Národní ústav pro neurologický výzkum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro neurologický výzkum, 5.1 EXCELES

722493, interní kód MU

Název: Natural Toxins and Drinking Water Quality - From Source to Tap (Akronym: NaToxAq)

Investor: Evropská unie, Natural Toxins and Drinking Water Quality - From Source to Tap, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

KUBÍČKOVÁ, Barbara, Šárka MARTINKOVÁ, Dáša BOHAČIAKOVÁ a Klára HILSCHEROVÁ. Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid. Toxicology Letters. CLARE: Elsevier, 2024, roč. 391, January 2024, s. 39-44. ISSN 0378-4274. Dostupné z: https://dx.doi.org/10.1016/j.toxlet.2023.12.004.

@article{2379118,
   author = {Kubíčková, Barbara and Martinková, Šárka and Bohačiaková, Dáša and Hilscherová, Klára},
   article_location = {CLARE},
   article_number = {January 2024},
   doi = {http://dx.doi.org/10.1016/j.toxlet.2023.12.004},
   keywords = {Developmental neurotoxicity; NSC; Retinoids; Differentiation; Anatoxin; Cyanotoxin},
   language = {eng},
   issn = {0378-4274},
   journal = {Toxicology Letters},
   title = {Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid},
   url = {https://www.sciencedirect.com/science/article/pii/S0378427423011116?via%3Dihub},
   volume = {391},
   year = {2024}
}

TY  - JOUR
ID  - 2379118
AU  - Kubíčková, Barbara - Martinková, Šárka - Bohačiaková, Dáša - Hilscherová, Klára
PY  - 2024
TI  - Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid
JF  - Toxicology Letters
VL  - 391
IS  - January 2024
SP  - 39-44
EP  - 39-44
PB  - Elsevier
SN  - 03784274
KW  - Developmental neurotoxicity
KW  - NSC
KW  - Retinoids
KW  - Differentiation
KW  - Anatoxin
KW  - Cyanotoxin
UR  - https://www.sciencedirect.com/science/article/pii/S0378427423011116?via%3Dihub
N2  - Cyanobacterial blooms are increasing in frequency and intensity globally, and impacting recreational waters as well as waters used for drinking water provisioning. They are sources of bioactive metabolites including retinoids and the neurotoxin anatoxin-a. Here, we investigated the effects of anatoxin-a on a differentiating in vitro human neural stem cell model previously characterised with retinoic acids. Effects on protein and gene expression upon exposure for 9 or 18 days to anatoxin-a alone or in co-exposure with all-trans retinoic acid were evaluated using a panel of neural and glial differentiation biomarkers. Anatoxin-a did not cause distinct developmental neuro-toxicity alone, or in co-exposure with retinoic acid. However, in line with its excitotoxicity, in co-exposure with 200 nM all-trans retinoic acid it reduced the differentiation of acetylcholinergic neuron subtypes in the culture at 1000 nM (highest tested concentration). While this could have substantial functional implications for the developing nervous system, there is no indication for developmental neurotoxicity beyond its (excito-)toxicity to acetylcholinergic neurons, which only occurred in co-exposure to all-trans retinoic acid.
ER  -

KUBÍČKOVÁ, Barbara, Šárka MARTINKOVÁ, Dáša BOHAČIAKOVÁ a Klára HILSCHEROVÁ. Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid. \textit{Toxicology Letters}. CLARE: Elsevier, 2024, roč.~391, January 2024, s.~39-44. ISSN~0378-4274. Dostupné z: https://dx.doi.org/10.1016/j.toxlet.2023.12.004.

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