J 2023

Fibroblast growth factors induce hepatic tumorigenesis post radiofrequency ablation

MARKEZANA, Aurelia, Mor PALDOR, Haixing LIAO, Muneeb AHMED, Elina ZORDE-KHVALEVSKY et. al.

Základní údaje

Originální název

Fibroblast growth factors induce hepatic tumorigenesis post radiofrequency ablation

Autoři

MARKEZANA, Aurelia, Mor PALDOR, Haixing LIAO, Muneeb AHMED, Elina ZORDE-KHVALEVSKY, Nir ROZENBLUM, Matthias STECHELE, Lukas SALVERMOSER, Flinn LAVILLE, Salome GOLDMANN, Nofar ROSENBERG, Tomáš ANDRAŠINA (703 Slovensko, domácí), Jens RICKE, Eithan GALUN a Shraga Nahum GOLDBERG

Vydání

Nature Scientific Reports, BERLIN, NATURE PORTFOLIO, 2023, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30224 Radiology, nuclear medicine and medical imaging

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 4.600 v roce 2022

Kód RIV

RIV/00216224:14110/23:00133674

Organizační jednotka

Lékařská fakulta

UT WoS

001095487500021

Klíčová slova anglicky

fibroblast growth factors; hepatic tumorigenesis post radiofrequency ablation

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 28. 2. 2024 14:16, Mgr. Tereza Miškechová

Anotace

V originále

Image-guided radiofrequency ablation (RFA) is used to treat focal tumors in the liver and other organs. Despite potential advantages over surgery, hepatic RFA can promote local and distant tumor growth by activating pro-tumorigenic growth factor and cytokines. Thus, strategies to identify and suppress pro-oncogenic effects of RFA are urgently required to further improve the therapeutic effect. Here, the proliferative effect of plasma of Hepatocellular carcinoma or colorectal carcinoma patients 90 min post-RFA was tested on HCC cell lines, demonstrating significant cellular proliferation compared to baseline plasma. Multiplex ELISA screening demonstrated increased plasma pro-tumorigenic growth factors and cytokines including the FGF protein family which uniquely and selectively activated HepG2. Primary mouse and immortalized human hepatocytes were then subjected to moderate hyperthermia in-vitro, mimicking thermal stress induced during ablation in the peri-ablational normal tissue. Resultant culture medium induced proliferation of multiple cancer cell lines. Subsequent non-biased protein array revealed that these hepatocytes subjected to moderate hyperthermia also excrete a similar wide spectrum of growth factors. Recombinant FGF-2 activated multiple cell lines. FGFR inhibitor significantly reduced liver tumor load post-RFA in MDR2-KO inflammation-induced HCC mouse model. Thus, Liver RFA can induce tumorigenesis via the FGF signaling pathway, and its inhibition suppresses HCC development.