Comparison of single and double pulse laser-induced breakdown
spectroscopy for the detection of biomolecules tagged with
photon-upconversion nanoparticles

J 2024

Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles

FARKA, Zdeněk, Karolína VYTISKOVÁ, Ekaterina MAKHNEVA, Eva ZIKMUNDOVÁ, Daniel HOLUB et. al.

Základní údaje

Originální název

Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles

Autoři

FARKA, Zdeněk (203 Česká republika, garant, domácí), Karolína VYTISKOVÁ (203 Česká republika), Ekaterina MAKHNEVA (643 Rusko, domácí), Eva ZIKMUNDOVÁ (203 Česká republika), Daniel HOLUB, Jakub BUDAY, David PROCHAZKA, Karel NOVOTNÝ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), Pavel POŘÍZKA (203 Česká republika) a Jozef KAISER (203 Česká republika)

Vydání

Analytica Chimica Acta, Elsevier, 2024, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2024.342418

UT WoS

001209691500001

Klíčová slova anglicky

Laser-induced breakdown spectroscopy; Double pulse; Tag-LIBS; Photon-upconversion nanoparticle; Immunoassay; Human serum albumin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 5. 2024 12:47, Mgr. Marie Šípková, DiS.

Anotace

V originále

Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially. The artificial implementation of nanoparticle labels (Tag-LIBS) enables the use of LIBS as a readout technique for immunochemical assays. However, one of the biggest challenges for LIBS to meet immunoassay readout standards is its sensitivity. This paper focuses on the improvement of LIBS sensitivity for the readout of nanoparticle-based immunoassays. First, the LIBS setup was optimized on photon-upconversion nanoparticle (UCNP) droplets deposited on the microtiter plate wells. Two collection optics systems were compared, with single pulse (SP) and collinear double pulse (DP) LIBS arrangements. By deploying the second laser pulse, the sensitivity was improved up to 30 times. The optimized SP and DP setups were then employed for the indirect detection of human serum albumin based on immunoassay with UCNP-based labels. Compared to our previous LIBS study, the detection limit was enhanced by two orders of magnitude, from 10 ng mL−1 to 0.29 ng mL−1. In addition, two other immunochemical methods were used for reference, based on the readout of upconversion luminescence of UCNPs and absorbance measurement with enzyme labels. Finally, the selectivity of the assay was tested and the practical potential of Tag-LIBS was demonstrated by the successful analysis of urine samples. In this work, we improved the sensitivity of the Tag-LIBS method by combining new labels based on UCNPs with the improved collection optics and collinear DP configuration. In the instrumental setup optimization, the DP LIBS showed better sensitivity and signal-to-noise ratio than SP. The optimizations allowed the LIBS readout to surpass the sensitivity of enzyme immunoassay, approaching the qualities of upconversion luminescence readout, which is nowadays a state-of-the-art readout technique.

Návaznosti

EF18_053/0016952, projekt VaV

Název: Postdoc2MUNI

GA22-27580S, projekt VaV

Název: Laserová spektroskopie v imunostanovení a zobrazování s nanometalickými značkami (Akronym: IMMUNOLIBS)

Investor: Grantová agentura ČR, Laserová spektroskopie v imunostanovení a zobrazování s nanometalickými značkami

Citovat

FARKA, Zdeněk, Karolína VYTISKOVÁ, Ekaterina MAKHNEVA, Eva ZIKMUNDOVÁ, Daniel HOLUB, Jakub BUDAY, David PROCHAZKA, Karel NOVOTNÝ, Petr SKLÁDAL, Pavel POŘÍZKA a Jozef KAISER. Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles. Analytica Chimica Acta. Elsevier, 2024, roč. 1299, April 2024, s. 1-8. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2024.342418.

@article{2380380,
   author = {Farka, Zdeněk and Vytisková, Karolína and Makhneva, Ekaterina and Zikmundová, Eva and Holub, Daniel and Buday, Jakub and Prochazka, David and Novotný, Karel and Skládal, Petr and Pořízka, Pavel and Kaiser, Jozef},
   article_number = {April 2024},
   doi = {http://dx.doi.org/10.1016/j.aca.2024.342418},
   keywords = {Laser-induced breakdown spectroscopy; Double pulse; Tag-LIBS; Photon-upconversion nanoparticle; Immunoassay; Human serum albumin},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles},
   url = {https://www.sciencedirect.com/science/article/abs/pii/S0003267024002198},
   volume = {1299},
   year = {2024}
}

TY  - JOUR
ID  - 2380380
AU  - Farka, Zdeněk - Vytisková, Karolína - Makhneva, Ekaterina - Zikmundová, Eva - Holub, Daniel - Buday, Jakub - Prochazka, David - Novotný, Karel - Skládal, Petr - Pořízka, Pavel - Kaiser, Jozef
PY  - 2024
TI  - Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles
JF  - Analytica Chimica Acta
VL  - 1299
IS  - April 2024
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00032670
KW  - Laser-induced breakdown spectroscopy
KW  - Double pulse
KW  - Tag-LIBS
KW  - Photon-upconversion nanoparticle
KW  - Immunoassay
KW  - Human serum albumin
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0003267024002198
N2  - Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially. The artificial implementation of nanoparticle labels (Tag-LIBS) enables the use of LIBS as a readout technique for immunochemical assays. However, one of the biggest challenges for LIBS to meet immunoassay readout standards is its sensitivity. This paper focuses on the improvement of LIBS sensitivity for the readout of nanoparticle-based immunoassays. First, the LIBS setup was optimized on photon-upconversion nanoparticle (UCNP) droplets deposited on the microtiter plate wells. Two collection optics systems were compared, with single pulse (SP) and collinear double pulse (DP) LIBS arrangements. By deploying the second laser pulse, the sensitivity was improved up to 30 times. The optimized SP and DP setups were then employed for the indirect detection of human serum albumin based on immunoassay with UCNP-based labels. Compared to our previous LIBS study, the detection limit was enhanced by two orders of magnitude, from 10 ng mL−1 to 0.29 ng mL−1. In addition, two other immunochemical methods were used for reference, based on the readout of upconversion luminescence of UCNPs and absorbance measurement with enzyme labels. Finally, the selectivity of the assay was tested and the practical potential of Tag-LIBS was demonstrated by the successful analysis of urine samples. In this work, we improved the sensitivity of the Tag-LIBS method by combining new labels based on UCNPs with the improved collection optics and collinear DP configuration. In the instrumental setup optimization, the DP LIBS showed better sensitivity and signal-to-noise ratio than SP. The optimizations allowed the LIBS readout to surpass the sensitivity of enzyme immunoassay, approaching the qualities of upconversion luminescence readout, which is nowadays a state-of-the-art readout technique.
ER  -

FARKA, Zdeněk, Karolína VYTISKOVÁ, Ekaterina MAKHNEVA, Eva ZIKMUNDOVÁ, Daniel HOLUB, Jakub BUDAY, David PROCHAZKA, Karel NOVOTNÝ, Petr SKLÁDAL, Pavel POŘÍZKA a Jozef KAISER. Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles. \textit{Analytica Chimica Acta}. Elsevier, 2024, roč.~1299, April 2024, s.~1-8. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2024.342418.

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