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@article{2380380,
author = {Farka, Zdeněk and Vytisková, Karolína and Makhneva, Ekaterina and Zikmundová, Eva and Holub, Daniel and Buday, Jakub and Prochazka, David and Novotný, Karel and Skládal, Petr and Pořízka, Pavel and Kaiser, Jozef},
article_number = {April 2024},
doi = {http://dx.doi.org/10.1016/j.aca.2024.342418},
keywords = {Laser-induced breakdown spectroscopy; Double pulse; Tag-LIBS; Photon-upconversion nanoparticle; Immunoassay; Human serum albumin},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0003267024002198},
volume = {1299},
year = {2024}
}
TY - JOUR
ID - 2380380
AU - Farka, Zdeněk - Vytisková, Karolína - Makhneva, Ekaterina - Zikmundová, Eva - Holub, Daniel - Buday, Jakub - Prochazka, David - Novotný, Karel - Skládal, Petr - Pořízka, Pavel - Kaiser, Jozef
PY - 2024
TI - Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles
JF - Analytica Chimica Acta
VL - 1299
IS - April 2024
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 00032670
KW - Laser-induced breakdown spectroscopy
KW - Double pulse
KW - Tag-LIBS
KW - Photon-upconversion nanoparticle
KW - Immunoassay
KW - Human serum albumin
UR - https://www.sciencedirect.com/science/article/abs/pii/S0003267024002198
N2 - Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially. The artificial implementation of nanoparticle labels (Tag-LIBS) enables the use of LIBS as a readout technique for immunochemical assays. However, one of the biggest challenges for LIBS to meet immunoassay readout standards is its sensitivity. This paper focuses on the improvement of LIBS sensitivity for the readout of nanoparticle-based immunoassays. First, the LIBS setup was optimized on photon-upconversion nanoparticle (UCNP) droplets deposited on the microtiter plate wells. Two collection optics systems were compared, with single pulse (SP) and collinear double pulse (DP) LIBS arrangements. By deploying the second laser pulse, the sensitivity was improved up to 30 times. The optimized SP and DP setups were then employed for the indirect detection of human serum albumin based on immunoassay with UCNP-based labels. Compared to our previous LIBS study, the detection limit was enhanced by two orders of magnitude, from 10 ng mL−1 to 0.29 ng mL−1. In addition, two other immunochemical methods were used for reference, based on the readout of upconversion luminescence of UCNPs and absorbance measurement with enzyme labels. Finally, the selectivity of the assay was tested and the practical potential of Tag-LIBS was demonstrated by the successful analysis of urine samples. In this work, we improved the sensitivity of the Tag-LIBS method by combining new labels based on UCNPs with the improved collection optics and collinear DP configuration. In the instrumental setup optimization, the DP LIBS showed better sensitivity and signal-to-noise ratio than SP. The optimizations allowed the LIBS readout to surpass the sensitivity of enzyme immunoassay, approaching the qualities of upconversion luminescence readout, which is nowadays a state-of-the-art readout technique.
ER -
FARKA, Zdeněk, Karolína VYTISKOVÁ, Ekaterina MAKHNEVA, Eva ZIKMUNDOVÁ, Daniel HOLUB, Jakub BUDAY, David PROCHAZKA, Karel NOVOTNÝ, Petr SKLÁDAL, Pavel POŘÍZKA a Jozef KAISER. Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles. \textit{Analytica Chimica Acta}. Elsevier, 2024, roč.~1299, April 2024, s.~1-8. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2024.342418.
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