Hyaluronic acid-based hydrogels with tunable mechanics improved
structural and contractile properties of cells

J 2024

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA et. al.

Základní údaje

Originální název

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Autoři

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL a Jan PŘIBYL

Vydání

Biomaterials Advances, Elsevier B.V. 2024, 2772-9508

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.bioadv.2024.213819

Klíčová slova anglicky

Hydrogels; Tunable properties; Hyaluronic acid; AFM; Holotomography; Actin structure; Mechanotransduction; HL-1; MEA

Štítky

14110513, 14110518

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 3. 2024 14:19, Mgr. Marie Šípková, DiS.

Anotace

V originále

Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LX22NPO5104, projekt VaV

Název: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Akronym: CarDia)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut pro léčbu metabolických a kardiovaskulárních onemocnění, 5.1 EXCELES

NU22J-08-00062, projekt VaV

Název: Mechanická a morfologická high-throughput fenotypizace nádorových buněk během smykového napětí: prediktor migračního a invazivního potenciálu

Investor: Ministerstvo zdravotnictví ČR, Mechanická a morfologická high-throughput fenotypizace nádorových buněk během smykového napětí: prediktor migračního a invazivního potenciálu, Podprogram 2 - juniorský - výzkumníci do 35 let

Citovat

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL a Jan PŘIBYL. Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells. Biomaterials Advances. Elsevier B.V., 2024, roč. 159, May 2024, s. 1-12. ISSN 2772-9508. Dostupné z: https://dx.doi.org/10.1016/j.bioadv.2024.213819.

@article{2381060,
   author = {Klimovič, Šimon and Beckerová, Deborah and Věžník, Jakub and Kabanov, Daniil and Lacina, Karel and Jelínková, Šárka and Gumulec, Jaromír and Rotrekl, Vladimír and Přibyl, Jan},
   article_number = {May 2024},
   doi = {http://dx.doi.org/10.1016/j.bioadv.2024.213819},
   keywords = {Hydrogels; Tunable properties; Hyaluronic acid; AFM; Holotomography; Actin structure; Mechanotransduction; HL-1; MEA},
   language = {eng},
   issn = {2772-9508},
   journal = {Biomaterials Advances},
   title = {Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells},
   url = {https://www.sciencedirect.com/science/article/pii/S2772950824000621},
   volume = {159},
   year = {2024}
}

TY  - JOUR
ID  - 2381060
AU  - Klimovič, Šimon - Beckerová, Deborah - Věžník, Jakub - Kabanov, Daniil - Lacina, Karel - Jelínková, Šárka - Gumulec, Jaromír - Rotrekl, Vladimír - Přibyl, Jan
PY  - 2024
TI  - Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells
JF  - Biomaterials Advances
VL  - 159
IS  - May 2024
SP  - 1-12
EP  - 1-12
PB  - Elsevier B.V.
SN  - 27729508
KW  - Hydrogels
KW  - Tunable properties
KW  - Hyaluronic acid
KW  - AFM
KW  - Holotomography
KW  - Actin structure
KW  - Mechanotransduction
KW  - HL-1
KW  - MEA
UR  - https://www.sciencedirect.com/science/article/pii/S2772950824000621
N2  - Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.
ER  -

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL a Jan PŘIBYL. Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells. \textit{Biomaterials Advances}. Elsevier B.V., 2024, roč.~159, May 2024, s.~1-12. ISSN~2772-9508. Dostupné z: https://dx.doi.org/10.1016/j.bioadv.2024.213819.

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