Markers of dental pulp stem cells in in vivo developmental
context

J 2023

Markers of dental pulp stem cells in in vivo developmental context

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA et. al.

Základní údaje

Originální název

Markers of dental pulp stem cells in in vivo developmental context

Autoři

ŠVANDOVÁ, Eva (203 Česká republika, domácí), Barbora VESELA (203 Česká republika), Adela KRATOCHVILOVA (203 Česká republika), Kateřina HOLOMKOVÁ (203 Česká republika, domácí), Veronika ORALOVA (203 Česká republika), Katerina DADAKOVA (203 Česká republika), Tom BURGER (203 Česká republika), Paul SHARPE (203 Česká republika), Herve LESOT (203 Česká republika) a Eva MATALOVA (203 Česká republika)

Vydání

Annals of Anatomy - Anatomischer Anzeiger, Munich, Elsevier, 2023, 0940-9602

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200 v roce 2022

Kód RIV

RIV/00216224:14110/23:00134419

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.aanat.2023.152149

UT WoS

001068775700001

Klíčová slova anglicky

Dental pulp; Development; Stem cell markers; In vivo

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 3. 2024 13:33, Mgr. Tereza Miškechová

Anotace

V originále

Teeth and their associated tissues contain several populations of mesenchymal stem cells, one of which is represented by dental pulp stem cells (DPSCs). These cells have mainly been characterised in vitro and numerous positive and negati ve markers for these cells have been suggested. To investigate the presence and localization of these molecules during development, forming dental pulp was examined using the mouse first mandibular molar as a model. The stages corresponding to postnatal (P) days 0, 7, 14, and 21 were investigated. The expression was monitored using customised PCR Arrays. Additionally, in situ localization of the key trio of markers (Cd73, Cd90, Cd105 coded by genes Nt5e, Thy1, Eng) was performed at prenatal and postnatal stages using immunohistochemistry. The expression panel of 24 genes assigned as in vitro markers of DPSCs or mesenchymal stem cells (MSCs) revealed their developmental dynamics during formation of dental pulp mesenchyme. Among the positive markers, Vcam1, Fgf2, Nes were identified as increasing and Cd44, Cd59b, Mcam, Alcam as decreasing between perinatal vs. postnatal stages towards adulthood. Within the panel of negative DPSC markers, Cd14, Itgb2, Ptprc displayed increased and Cd24a decreased levels at later stages of pulp formation. Within the key trio of markers, Nt5e did not show any significant expression difference within the investigated period. Thy1 displayed a strong decrease between P0 and P7 while Eng increased between these stages. In situ localization of Cd73, Cd90 and Cd105 showed them overlap in differentiated odontoblasts and in the sub-odontoblastic layer that is speculated to host odontoblast progenitors. The highly prevalent expression of particularly Cd73 and Cd90 opens the question of potential multiple functions of these molecules. The results from this study add to the in vitro based knowledge by showing dynamics in the expression of DPSC/MSC markers during dental pulp formation in an in vivo context and thus with respect to the natural environment important for commitment of stem cells. & COPY; 2023 Published by Elsevier GmbH.

Návaznosti

GA21-21409S, projekt VaV

Název: Fyziologické vlastnosti a funkce kmenových buněk vztahujících se k dentici se zaměřením na kontext in vivo

Investor: Grantová agentura ČR, Fyziologické vlastnosti a funkce kmenových buněk vztahujících se k dentici se zaměřením na kontext in vivo

Citovat

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA, Katerina DADAKOVA, Tom BURGER, Paul SHARPE, Herve LESOT a Eva MATALOVA. Markers of dental pulp stem cells in in vivo developmental context. Annals of Anatomy - Anatomischer Anzeiger. Munich: Elsevier, 2023, roč. 250, October 2023, s. 1-10. ISSN 0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2023.152149.

@article{2382297,
   author = {Švandová, Eva and Vesela, Barbora and Kratochvilova, Adela and Holomková, Kateřina and Oralova, Veronika and Dadakova, Katerina and Burger, Tom and Sharpe, Paul and Lesot, Herve and Matalova, Eva},
   article_location = {Munich},
   article_number = {October 2023},
   doi = {http://dx.doi.org/10.1016/j.aanat.2023.152149},
   keywords = {Dental pulp; Development; Stem cell markers; In vivo},
   language = {eng},
   issn = {0940-9602},
   journal = {Annals of Anatomy - Anatomischer Anzeiger},
   title = {Markers of dental pulp stem cells in in vivo developmental context},
   url = {https://www.sciencedirect.com/science/article/pii/S0940960223001048?via%3Dihub},
   volume = {250},
   year = {2023}
}

TY  - JOUR
ID  - 2382297
AU  - Švandová, Eva - Vesela, Barbora - Kratochvilova, Adela - Holomková, Kateřina - Oralova, Veronika - Dadakova, Katerina - Burger, Tom - Sharpe, Paul - Lesot, Herve - Matalova, Eva
PY  - 2023
TI  - Markers of dental pulp stem cells in in vivo developmental context
JF  - Annals of Anatomy - Anatomischer Anzeiger
VL  - 250
IS  - October 2023
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 09409602
KW  - Dental pulp
KW  - Development
KW  - Stem cell markers
KW  - In vivo
UR  - https://www.sciencedirect.com/science/article/pii/S0940960223001048?via%3Dihub
N2  - Teeth and their associated tissues contain several populations of mesenchymal stem cells, one of which is represented by dental pulp stem cells (DPSCs). These cells have mainly been characterised in vitro and numerous positive and negati ve markers for these cells have been suggested. To investigate the presence and localization of these molecules during development, forming dental pulp was examined using the mouse first mandibular molar as a model. The stages corresponding to postnatal (P) days 0, 7, 14, and 21 were investigated. The expression was monitored using customised PCR Arrays. Additionally, in situ localization of the key trio of markers (Cd73, Cd90, Cd105 coded by genes Nt5e, Thy1, Eng) was performed at prenatal and postnatal stages using immunohistochemistry. The expression panel of 24 genes assigned as in vitro markers of DPSCs or mesenchymal stem cells (MSCs) revealed their developmental dynamics during formation of dental pulp mesenchyme. Among the positive markers, Vcam1, Fgf2, Nes were identified as increasing and Cd44, Cd59b, Mcam, Alcam as decreasing between perinatal vs. postnatal stages towards adulthood. Within the panel of negative DPSC markers, Cd14, Itgb2, Ptprc displayed increased and Cd24a decreased levels at later stages of pulp formation. Within the key trio of markers, Nt5e did not show any significant expression difference within the investigated period. Thy1 displayed a strong decrease between P0 and P7 while Eng increased between these stages. In situ localization of Cd73, Cd90 and Cd105 showed them overlap in differentiated odontoblasts and in the sub-odontoblastic layer that is speculated to host odontoblast progenitors. The highly prevalent expression of particularly Cd73 and Cd90 opens the question of potential multiple functions of these molecules. The results from this study add to the in vitro based knowledge by showing dynamics in the expression of DPSC/MSC markers during dental pulp formation in an in vivo context and thus with respect to the natural environment important for commitment of stem cells. & COPY; 2023 Published by Elsevier GmbH.
ER  -

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA, Katerina DADAKOVA, Tom BURGER, Paul SHARPE, Herve LESOT a Eva MATALOVA. Markers of dental pulp stem cells in in vivo developmental context. \textit{Annals of Anatomy - Anatomischer Anzeiger}. Munich: Elsevier, 2023, roč.~250, October 2023, s.~1-10. ISSN~0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2023.152149.

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