Stimuli-Responsive Membrane Anchor Peptide Nanofoils for
Tunable Membrane Association and Lipid Bilayer Fusion

J 2022

Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion

NAGARAJ, Vignesh Udyavara, Tunde JUHASZ, Mayra QUEME-PENA, Imola Cs SZIGYARTO, Dora BOGDAN et. al.

Základní údaje

Originální název

Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion

Autoři

Vydání

ACS APPLIED MATERIALS & INTERFACES, WASHINGTON, AMER CHEMICAL SOC, 2022, 1944-8244

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21000 2.10 Nano-technology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.500

Kód RIV

RIV/00216224:90127/22:00133747

Organizační jednotka

CIISB II

DOI

http://dx.doi.org/10.1021/acsami.2c11946

UT WoS

000894035600001

Klíčová slova anglicky

self-assembly; liposomes; membrane activity; spiropyran; peptide bilayer; lipid bilaye r fusion

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 4. 2024 13:03, Mgr. Michal Petr

Anotace

V originále

Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into similar to 3.5 nm thick, foil-like peptide bilayer morphologies. Photo-isomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

NAGARAJ, Vignesh Udyavara, Tunde JUHASZ, Mayra QUEME-PENA, Imola Cs SZIGYARTO, Dora BOGDAN, Andras WACHA, Judith MIHALY, Lorand ROMANSZKI, Zoltan VARGA, Joakim ANDREASSON, Istvan MANDITY a Tamas BEKE-SOMFAI. Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion. ACS APPLIED MATERIALS & INTERFACES. WASHINGTON: AMER CHEMICAL SOC, 2022, roč. 14, č. 50, s. 55320-55331. ISSN 1944-8244. Dostupné z: https://dx.doi.org/10.1021/acsami.2c11946.

@article{2382877,
   author = {Nagaraj, Vignesh Udyavara and Juhasz, Tunde and QuemeandPena, Mayra and Szigyarto, Imola Cs and Bogdan, Dora and Wacha, Andras and Mihaly, Judith and Romanszki, Lorand and Varga, Zoltan and Andreasson, Joakim and Mandity, Istvan and BekeandSomfai, Tamas},
   article_location = {WASHINGTON},
   article_number = {50},
   doi = {http://dx.doi.org/10.1021/acsami.2c11946},
   keywords = {self-assembly; liposomes; membrane activity; spiropyran; peptide bilayer; lipid bilaye r fusion},
   language = {eng},
   issn = {1944-8244},
   journal = {ACS APPLIED MATERIALS & INTERFACES},
   title = {Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion},
   url = {https://pubs.acs.org/doi/10.1021/acsami.2c11946},
   volume = {14},
   year = {2022}
}

TY  - JOUR
ID  - 2382877
AU  - Nagaraj, Vignesh Udyavara - Juhasz, Tunde - Queme-Pena, Mayra - Szigyarto, Imola Cs - Bogdan, Dora - Wacha, Andras - Mihaly, Judith - Romanszki, Lorand - Varga, Zoltan - Andreasson, Joakim - Mandity, Istvan - Beke-Somfai, Tamas
PY  - 2022
TI  - Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion
JF  - ACS APPLIED MATERIALS & INTERFACES
VL  - 14
IS  - 50
SP  - 55320-55331
EP  - 55320-55331
PB  - AMER CHEMICAL SOC
SN  - 19448244
KW  - self-assembly
KW  - liposomes
KW  - membrane activity
KW  - spiropyran
KW  - peptide bilayer
KW  - lipid bilaye r fusion
UR  - https://pubs.acs.org/doi/10.1021/acsami.2c11946
N2  - Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into similar to 3.5 nm thick, foil-like peptide bilayer morphologies. Photo-isomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.
ER  -

NAGARAJ, Vignesh Udyavara, Tunde JUHASZ, Mayra QUEME-PENA, Imola Cs SZIGYARTO, Dora BOGDAN, Andras WACHA, Judith MIHALY, Lorand ROMANSZKI, Zoltan VARGA, Joakim ANDREASSON, Istvan MANDITY a Tamas BEKE-SOMFAI. Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion. \textit{ACS APPLIED MATERIALS \&{}amp; INTERFACES}. WASHINGTON: AMER CHEMICAL SOC, 2022, roč.~14, č.~50, s.~55320-55331. ISSN~1944-8244. Dostupné z: https://dx.doi.org/10.1021/acsami.2c11946.

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