Další formáty:
BibTeX
LaTeX
RIS
@article{2383577, author = {Mitrevska, Katerina and Rodrigo, Miguel Angel Merlos and Cernei, Natalia and Michalkova, Hana and Splichal, Zbynek and Hynek, David and Zitka, Ondrej and Heger, Zbynek and Kopel, Pavel and Adam, Vojtech and Milosavljevic, Vedran}, article_location = {AMSTERDAM}, article_number = {Apr}, doi = {http://dx.doi.org/10.1016/j.mtbio.2023.100570}, keywords = {Platinum nanoparticles; Cisplatin; CAM assay; Breast cancer; Amino acids metabolism; TCA cycle}, language = {eng}, issn = {2590-0064}, journal = {Materials Today Bio}, title = {Chick chorioallantoic membrane (CAM) assay for the evaluation of the antitumor and antimetastatic activity of platinum-based drugs in association with the impact on the amino acid metabolism}, url = {https://www.sciencedirect.com/science/article/pii/S2590006423000303?via%3Dihub}, volume = {19}, year = {2023} }
TY - JOUR ID - 2383577 AU - Mitrevska, Katerina - Rodrigo, Miguel Angel Merlos - Cernei, Natalia - Michalkova, Hana - Splichal, Zbynek - Hynek, David - Zitka, Ondrej - Heger, Zbynek - Kopel, Pavel - Adam, Vojtech - Milosavljevic, Vedran PY - 2023 TI - Chick chorioallantoic membrane (CAM) assay for the evaluation of the antitumor and antimetastatic activity of platinum-based drugs in association with the impact on the amino acid metabolism JF - Materials Today Bio VL - 19 IS - Apr SP - 1-14 EP - 1-14 PB - Elsevier SN - 25900064 KW - Platinum nanoparticles KW - Cisplatin KW - CAM assay KW - Breast cancer KW - Amino acids metabolism KW - TCA cycle UR - https://www.sciencedirect.com/science/article/pii/S2590006423000303?via%3Dihub N2 - The combination of in ovo and ex ovo chorioallantoic membrane (CAM) assay provides an excellent platform which extends its relevance in studying carcinogenesis to the field of screening of anticancer activity of platinum nanoparticles (PtNPs) and further study of the amino acids' fluctuations in liver and brain. PtNPs are promising candidates for replacing cisplatin (CDDP); however, insufficient data of their antitumor efficiency and activity on the cancer-related amino acid metabolism are available, and the assessment of the in vivo performance has barely scratched the surface. Herein, we used CAM assay as in vivo model for screening of novel therapeutic modalities, and we conducted a comparative study of the effects of CDDP and polyvinylpyrrolidone coated PtNPs on MDA-MB-231 breast cancer xenograft. PtNPs showed a higher efficiency to inhibit the tumor growth and metastasis compared to CDDP. The amino acids profiling in the MDA-MB-231 cells revealed that the PtNPs had an overall depleting effect on the amino acids content. Noteworthy, more side effects to amino acid metabolism were deduced from the depletion of the amino acids in tumor, brain, and liver upon CDDP treatment. Different sets of enzymes of the tricarboxylic acid (TCA) cycle were targeted by PtNPs and CDDP, and while mRNA encoding multiple enzymes was downregulated by PtNPs, the treatment with CDDP affected only two TCA enzymes, indicating a different mechanism of action. Taken together, CAM assay represents and invaluable model, demonstrating the PtNPs capability of repressing angiogenesis, decrease amino acid contents and disrupt the TCA cycle. ER -
MITREVSKA, Katerina, Miguel Angel Merlos RODRIGO, Natalia CERNEI, Hana MICHALKOVA, Zbynek SPLICHAL, David HYNEK, Ondrej ZITKA, Zbynek HEGER, Pavel KOPEL, Vojtech ADAM a Vedran MILOSAVLJEVIC. Chick chorioallantoic membrane (CAM) assay for the evaluation of the antitumor and antimetastatic activity of platinum-based drugs in association with the impact on the amino acid metabolism. \textit{Materials Today Bio}. AMSTERDAM: Elsevier, 2023, roč.~19, Apr, s.~1-14. ISSN~2590-0064. Dostupné z: https://dx.doi.org/10.1016/j.mtbio.2023.100570.
|