| BLAHUT, Jan, Matthias J BRANDL, Riddhiman SARKAR, Bernd REIF a Zdenek TOS. Optimal control derived sensitivity-enhanced CA-CO mixing sequences for MAS solid-state NMR - Applications in sequential protein backbone assignments. JOURNAL OF MAGNETIC RESONANCE OPEN. AMSTERDAM: ELSEVIER, 2023, roč. 16-17, Dec, s. 1-9. ISSN 2666-4410. Dostupné z: https://dx.doi.org/10.1016/j.jmro.2023.100122. |
Další formáty:
BibTeX
LaTeX
RIS
@article{2383600,
author = {Blahut, Jan and Brandl, Matthias J and Sarkar, Riddhiman and Reif, Bernd and Tos, Zdenek},
article_location = {AMSTERDAM},
article_number = {Dec},
doi = {http://dx.doi.org/10.1016/j.jmro.2023.100122},
keywords = {FULLY PROTONATED PROTEINS; CROSS-POLARIZATION; SPECTROSCOPY; RESONANCE; DYNAMICS SIMULATION; NCO},
language = {eng},
issn = {2666-4410},
journal = {JOURNAL OF MAGNETIC RESONANCE OPEN},
title = {Optimal control derived sensitivity-enhanced CA-CO mixing sequences for MAS solid-state NMR - Applications in sequential protein backbone assignments},
url = {https://www.sciencedirect.com/science/article/pii/S2666441023000304?via%3Dihub},
volume = {16-17},
year = {2023}
}
TY - JOUR
ID - 2383600
AU - Blahut, Jan - Brandl, Matthias J - Sarkar, Riddhiman - Reif, Bernd - Tos, Zdenek
PY - 2023
TI - Optimal control derived sensitivity-enhanced CA-CO mixing sequences for MAS solid-state NMR - Applications in sequential protein backbone assignments
JF - JOURNAL OF MAGNETIC RESONANCE OPEN
VL - 16-17
IS - Dec
SP - 1-9
EP - 1-9
PB - ELSEVIER
SN - 26664410
KW - FULLY PROTONATED PROTEINS
KW - CROSS-POLARIZATION
KW - SPECTROSCOPY
KW - RESONANCE
KW - DYNAMICS SIMULATION
KW - NCO
UR - https://www.sciencedirect.com/science/article/pii/S2666441023000304?via%3Dihub
N2 - We have recently introduced optimal-control derived pulse sequences for sensitivity-enhanced heteronuclear correlation NMR experiments of solid proteins. Preservation of equivalent coherence transfer pathways using transverse-mixing pulses (TROP) in multidimensional pulse schemes allows to increase the sensitivity of the experiments by more than a factor of root 2 per each indirect dimension. In this article, we present homonuclear CA-CO transverse-mixing elements (homoTROP) that are based on dipolar interactions and achieve similar gains as the heteronuclear TROP pulses described previously. Both transfer elements were subsequently implemented in 3D se-hCAcoNH and se-hCOcaNH, that together with the previously introduced 3D se-hCANH and se-hCONH experiments yield a complete set of sensitivity-enhanced protein backbone assignment experiments. In contrast to the J-coupling based methods that are used at fast (60 kHz) and ultrafast MAS (>100 kHz), the homoTROP experiments employ about 10-times shorter mixing times making use of the larger magnitude of the dipolar coupling in comparison to the J couplings. The experiments are demonstrated using a microcrystalline, perdeuterated sample of the chicken alpha-spectrin SH3 domain in which all exchangeable sites are fully back-substituted with protons. We evaluated the gains in efficiency in all experiments site-specifically observing that the se-hCAcoNH and se-hCOcaNH experiments yield an increase in sensitivity by a factor of 1.36 +/- 0.09 and at least a factor of 1.8 with respect to the conventional hcoCAcoNH and hCOcaNH J-based experiments.
ER -
BLAHUT, Jan, Matthias J BRANDL, Riddhiman SARKAR, Bernd REIF a Zdenek TOS. Optimal control derived sensitivity-enhanced CA-CO mixing sequences for MAS solid-state NMR - Applications in sequential protein backbone assignments. \textit{JOURNAL OF MAGNETIC RESONANCE OPEN}. AMSTERDAM: ELSEVIER, 2023, roč.~16-17, Dec, s.~1-9. ISSN~2666-4410. Dostupné z: https://dx.doi.org/10.1016/j.jmro.2023.100122.
|
